The effect of in vitro royal jelly provision on adhesion of Pseudomonas aeruginosa

https://doi.org/10.22146/majkedgiind.30221

Sifra Kristina Hartono(1*), Tetiana Haniastuti(2), Heni Susilowati(3), Juni Handajani(4), Alma Linggar Jonarta(5)

(1) Dentistry Study Program, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(2) Department of Oral Biology, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(3) Department of Oral Biology, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(4) Department of Oral Biology, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(5) Department of Oral Biology, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author

Abstract


Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic bacterium, which could aggressively infect immunocompromised patients and thus, cause high mortality rate. In addition, P. aeruginosa in oropharynx could be aspirated and cause ventilator associated pneumonia. Royal jelly is one of bee’s products that has been used for therapeutic needs including antibacteria. Adherence factor of P. aeruginosa were flagelum, pili and lectin. The aim of the study was to determine the effect of royal jelly to P. aeruginosa adhesion. Suspension of P. aeruginosa (ATCC® 27853) was incubated at 37 °C for 18 h. Treatment groups were exposed to royal jelly with several concentrations, 2%, 4%, 6%; while distilled water was being used as negative control. Bacterial adhesion test was determined using spectrophotometer λ = 600 nm to measure optical density values of adhered bacterial suspension in tubes. The result of one-way ANOVA showed significant differences (p<0.05) of optical density values among groups indicating that royal jelly affected the bacterial adhesion. LSD results showed significant difference of optical density values between 2%, 4%, and 6% royal jelly compared to distilled water. Six percent of royal jelly had the least optical density value compared to the other groups. In conclusion, royal jelly has the ability to decrease adhesion of P. aeruginosa. Six percent of royal jelly has better ability to decrease adhesion of P. aeruginosa than other concentrations.

Keywords


bacterial adhesion; Pseudomonas aeruginosa; royal jelly

Full Text:

PDF2


References

Seshadri S. Reversal of Pseudomonas infections by plant extracts [Internet]. Nirma University: LAP Lambert Academic Publishing GmbH & Co. KG; 2010 [cited 2014 November 25]. Available from Reseachgate: https://www.researchgate.net/publication/216844397_Reversal_of_Pseudomonas_infections_by_plant_extracts

2. Department of Health UK. HTM 04-01 - Addendum: Pseudomonas aeruginosa –
advice for augmented care units. 2013 [cited 2016 June 5]. Available from Department of Health United Kingdom: https://www.gov.uk/government/uploads/
system/uploads/attachment_data/file/140105/Health_Technical_Memorandum_04-01_Addendum.pdf

3. Vitkauskienė A, Skrodenienė E, Dambrauskienė A, Macas A, Sakalauskas
R. Pseudomonas aeruginosa bacteremia: resistance to antibiotics, risk factors, and
patient mortality. Medicina (Kaunas). 2010; 46(7):490-495. doi: 10.3390/medicina46070071


4. Lawley R, Curtis L, Davis J. The Food Safety Hazard Guidebook. United Kingdom: RSC Publishing; 2012.

5. Barben J, Schmid J. Dental units as infection sources of Pseudomonas aeruginosa. Eur Respir J. 2008; 32(4): 1122-1123. doi: 10.1183/09031936.00072808


6. Iversen BG. Contaminated mouth swabs caused a multi-hospital outbreak of
Pseudomonas aeruginosa infection. J Oral Microbiol. 2010; 2: 5123-5126.
doi: 10.3402/jom.v2i0.5123

7. Hunter JD. Ventilator associated pneumonia. Postgrad Med J. 2005; 82(965): 172–178.


8. Genuit T, Bochicchio G, Napolitano LM, McCarter RJ, Roghman MC. Prophylactic
chlorhexidine oral rinse decreases ventilatorassociated pneumonia in surgical ICU patients. Surg Infect. 2001; 2(1): 5-18. doi: 10.1089/109629601750185316

9. Grishin AV, Krivozubov MS, Karyagina AS, Gintsburg AL. Pseudomonas aeruginosa lectins as targets for novel antibacterials. Acta Naturae. 2015; 7(2): 29–41.


10. Oliveira MRTR, Napimoga MH, Cogo K, Gonçalves RB, Macedo MLR, Freire MGM, Groppo FC. Inhibition of bacterial adherence on saliva-coated through plant lectins. Braz J Oral Sci. 2007; 49(2): 141-145.

11. AM, Orth K. Targeting the bacteria–host interface. Virulence. 2013; 4(4): 284-294. doi: 10.4161/viru.24606

12. Comolli JC, Waite LL, Mostov KE, Engel JN. Pili Bending to Asialo–GM 1 on Epithelial cells can mediate cytotoxicity or bacterial internalisation by Pseudomonas aeruginosa. Infect Immun. 1999; 67(7): 3207–3214.

13. Woods DE, Straus DC, Johanson Jr WG, Bass JA. Factors influencing the adherence of Pseudomonas aeruginosa to mammalian buccal epithelial cells. Rev Infect Dis 1983; 5(5): 846-851.

14. Alendejani T, Marsan J, Ferris W, Slinger R, Chan F. Effectiveness of honey on
Staphylococcus aureus and Pseudomonas aeruginosa biofilms. Arch Otolaryngol Head Neck Surg. 2009; 141(1): 114-118. doi: 10.1016/j.otohns.2009.01.005

15. Boukraa L. Additive activity of royal jelly and honey against Pseudomonas aeruginosa. Altern Med Rev. 2008; 13(4): 330-333.

16. Rachmaninov O, Zinger-Yosovich KD, GilboaGalber N. Blocking Pseudomonas aeruginosa, Chromobacterium violaceum, and Ralstonia solanacearum adhesion by Fruit Glycans. J Nutrition Health Food Sci 2014; 2(3): 1-9.
doi: 10.15226/jnhfs.2014.00123

17. Nostro A, Cannatelli MA, Crisafi G, Musolino AD, Procopio F, Alonzo V. Modifications of hydrophobicity, in vitro adherence and cellular aggregation of Streptococcus mutans by Helichrysum italicum extract. Lett Appl Microbiol. 2004; 38: 423-427. doi: 10.1111/j.1472-765X.2004.01509.x

18. Miguel MG, El-Guendouz S. Volatile Compounds of Royal Jelly in: AlvarezSuarez J. (eds) Bee Products - Chemical and Biological Properties.
Switzerland: Springer; 2017. 191-197.

19. Bărnuţiu LI, Mărghitaş LA, Dexmirean DS, Mihai CM, Bobiş O. Chemical composition and antimicrobial activity of royal jelly – Review. SPASB. 2010; 44(2): 67-72.



DOI: https://doi.org/10.22146/majkedgiind.30221

Article Metrics

Abstract views : 1988 | views : 1688

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2019 Majalah Kedokteran Gigi Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

 View My Stats


real time web analytics