* Corresponding Author
Morphological Structure of the Tongue of Gekko gecko in Yogyakarta, Indonesia
https://doi.org/10.22146/jtbb.90995 Khairunnisa Aqiilah(1), Teguh Budipitojo(2), Hery Wijayanto(3), Vista Budiariati(4), Tri Wahyu Pangestiningsih(5), Ariana Ariana(6), Golda Rani Saragih(7), Ulayatul Kustiati(8), Hevi Wihadmadyatami(9*)
(1) Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(2) Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(3) Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(4) Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(5) Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(6) Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(7) Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(8) Laboratory of Veterinary Pharmacology, Faculty of Veterinary Medicine, Universitas Brawijaya, East Java, Indonesia, 65151
(9) Department of Anatomy, Faculty of Veterinary Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(*) Corresponding Author
Abstract
Gekko gecko is a member of the order Squamata from the family Gekkonidae and mainly feeds on small insects. This study aims to determine the morphology of the tongue of Gekko gecko through Scanning Electron Microscopy (SEM), hematoxylin–eosin (HE) staining, and immunohistochemistry (IHC). Six adult Gekko gecko were obtained from the Special Region of Yogyakarta, and Gekko gecko tongue samples were stored in SEM fixative solution and then observed with SEM. For histochemical and IHC staining, tongue samples were processed into paraffin blocks and cut into 8 µm-thick sections. The SEM revealed three types of papillae: dome-shaped papillae at the apex, fan-shaped papillae at the corpus, and scale–like papillae at the radix. Histological observations showed that the tongue of the Gekko gecko was composed of tunica mucosa and tunica muscularis, and goblet cells were present in the lamina of the epithelial mucosa. Meanwhile, no taste buds were found. Immunoreactivity against PGP 9.5 was observed on the tunica muscularis of the apex, corpus, and radix. Taken together, this study provides new insight into the tongue morphology of Gekko gecko and is dominated by mechanical papillae on the tongue surface.
Keywords
Full Text:
PDFReferences
Abbate, F. et al., 2020. The tongue of Leopard Gecko (Eublepharis macularius): LM, SEM and confocal laser study. Anatomia Histologia Embryologia, 49(1), pp.51-59. doi: 10.1111/ahe.12483.
Al-Fartwsy, A.R., Al-Shuaily, E.H. & Al-Kubaisi, Z.A., 2016. Morphological, Histological and Ultrustructural Study of the Tongue in House Geecko (Hemidactylus flaviviridis) Lizard. Iraqi Journal of Biotechnology, 15(1), pp.1–11.
Bayoumi, S.S., Abd-Elhameed, A.E. & Mohamed, E.S.M., 2011. Comparative studies on the dorsal lingual surface of two Egyptian squamate reptile with two different feeding habits. The Egyptian Journal of Experimental Biology (Zoology), 7(2), pp.203–211. doi: 10.5455/egyjebb.20230607015627.
Carranza, S. & Arnold, E.N., 2006. Systematics, biogeography, and evolution of Hemi-dactylus Gekko (Reptilia: Gekkonidae) elucidated using mitochondrial DNA sequences. Molecular Phylogenetics and Evolution, 38(2), pp.531–545. doi: 10.1016/j.ympev.2005.07.012.
Cahyani, N.K.D. et al., 2023. Population study of tokay gecko (Gekko gecko) in Bali Province, Indonesia (Studi populasi Tokek Rumah (Gekko gecko) di Provinsi Bali, Indonesia). Jurnal Biologi Indonesia, 19(2), pp.125-133. doi: 10.47349/jbi/19022023/125.
Čížek, P. et al., 2017. Light and scanning electron microscopy of the tongue of a degu (Octodon degus). Anatomical Science International, 92(4), pp.493-499. doi: 10.1007/s12565-016-0346-x.
El-Mansi, A.A. et al., 2020. Structural and functional characterization of the tongue and digestive tractof Psammophis sibilans (Squamata, Lamprophiidae): Adaptive strategies for foraging and feeding behaviors. Microscopy and Microanalysis, 26(3), pp.524-541. doi: 10.1017/S1431927620001312
Emura, S. & Bakary, N.E.R.E., 2014. Morphology of the lingual papillae of egyptian buffalo (Bubalus bubalis). Okajimas Folia Anatomica Japonica, 91(1), pp.13-17. doi: 10.2535/ofaj.91.13.
Erdoğan, S., Arias, S.V. & Pérez, W., 2015. Morphology of the lingual surface of south American fur seal (Arctocephalus australis) and sea lion (Otaria flavescens). Microscopy Research and Technique, 78(2), pp.140-147. doi: 10.1002/jemt.22456.
Filoramo, N.I. & Schwenk, K., 2009. The mechanism of chemical delivery to the vomeronasal organs in squamate reptiles: A comparative morphological approach. Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, 311(1), pp.20–34. doi: 10.1002/jez.492.
Gewily, D.I. et al., 2021. Ultrastructural comparison between the tongue of two reptilian species endemic in Egyptian fauna; Bosc's fringe‐toed lizard Acanthodactylus boskianus and Sinai fan‐fingered gecko Ptyodactylus guttatus. Microscopy Research and Technique, 84(9), pp.1977-1991. doi: 10.1002/jemt.23753.
Hildyard, A., 2001. Endangered wildlife and plants of the world (Vol. 5), Marshall Cavendish Corporation.
Iwasaki, S.I. & Miyata, K., 1985. Scanning electron microscopy of the lingual dorsal surface of the Japanese lizard, Takydromus tachydromoides. Okajimas Folia Anatomica Japonica, 62(1), pp.15–26. doi: 10.2535/ofaj1936.62.1_15.
Iwasaki S., 1990. Fine structure of the dorsal lingual epithelium of the lizard, Gekko japonicus (Lacertilia, Gekkonidae). American Journal of Anatomy, 187(1), pp.12-20. doi: 10.1002/aja.1001870103.
Iwasaki, S.I., Yoshizawa, H. & Kawahara, I., 1996. Three dimensional ultrasctructure of the surface of the tongue of the rat snake, Elaphe climacophora. The Anatomical Record, 245(1), pp.9–12. doi: 10.1002/(sici)1097-0185(199605)245:1%3C9::aid-ar2%3E3.0.co;2-v.
Jackowiak, H. & Godynicki, S., 2007. Light and scanning electron microscopic study on the structure of the lingual papillae of the feathertail glider (Acrobates pygmeus, Burramyidae, Marsupialia). The Anatomical Record, 290(11), pp.1355-1365. doi:10.1002/ar.20606.
Jamniczky, H.A. et al., 2009. Morphology and histology of the tongue and oral chamber of Eublepharis macularius (Squamata: Gekkonidae), with special reference to the foretongue and its role in fluid uptake and transport. Evolutionary Biology, 36(4), pp.11-15. doi: 10.1007/s11692-009-9072-9.
Kusrini, M.D., 2019. Metode Survei dan Penelitian Herpetofauna, IPB Press.
Manley, G. & Kraus, J., 2010. Exceptional high frequency hearing and matched vocalizations in Australian pygopod geckos. Journal of Experimental Biology, 213(11), pp.1876-1885. doi: 10.1242/jeb.040196.
Partasasmita, R., Iskandar, J. & Malone, N., 2016. Karangwangi people’s (South Cianjur, West Java, Indonesia) local knowledge of species, forest utilization and wildlife conservation. Biodiversitas, 17(1), pp.8-12. doi: 10.13057/biodiv/d170123.
Pastor et al., 2017. Ultrastructure of lingual papillae in common chimpanzee (Pan troglodytes) foetus, newborn and adult specimens. Anatomia Histologia Embryologia, 46(5), pp.431-438. doi: 10.1111/ahe.12287.
Pathak, S.K. et al., 2015. Morphological characterization of Bengal Monitor Lizard (Varanus bengalensis). Animal Science Reporter, 9(2), pp.70-74.
Rocha et al., 2015. Occurrence of the tokay gecko Gekko gecko (Linnaeus 1758) (Squamata, Gekkonidae), an exotic species in southern Brazil. Herpetology Notes, 8, pp.8-10. doi: 10.1163/15685381-440405SEH.
Schwenk, K., 1994. Why Snakes Have Forked Tongues. Science, 263(5153), pp.1573-1577. doi: 10.1126/science.263.5153.1573.
Schwenk, K., 2000. Feeding: Form, Function, and Evolution in Tetrapod Vertebrates, Academic Press.
Sheren, A.Z.A., Nasr, E.S. & Hassan, S.S., 2018. Light and scanning electron microscopic observations on the tongue of Nile monitor, Varanus niloticus niloticus. International Journal of Advanced Research in Biological Sciences, 5(4), pp.1–11. doi: 10.22192/ijarbs.2018.05.04.001
Smith, K.K. & Mackay, K.A., 1990. The morphology of the intrinsic tongue Musculature in snakes (Reptilia, Ophidia): functional and phylogenetic implications. Journal of Morphology, 205(3), pp.307–324. doi: 10.1002/jmor.1052050306.
Toprak, B., 2023. Investigations on the light and scanning electron microscopic structure of the lingual papillae in the angora goat (Capra hircus): ii. mechanical papillae. Anatomia Histologia Embryologia, 52(2), pp.327-335. doi: 10.1111/ahe.12890.
Wannaprasert, T., Phantuma-opas, P. & Jindatip, D., 2019. Morphology and microstructure of the tongue of the lesser bamboo rat (Cannomys badius). Acta Zoologica, 101(3), pp.282-291. doi: 10.1111/azo.12293.
DOI: https://doi.org/10.22146/jtbb.90995
Article Metrics
Abstract views : 84
| 
views : 4021
Refbacks
- There are currently no refbacks.
Copyright (c) 2024 Journal of Tropical Biodiversity and Biotechnology
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Editoral address:
Faculty of Biology, UGM
Jl. Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia
ISSN: 2540-9581 (online)