* Corresponding Author
Detection of Entomological Origin of Honey Sold in Indonesia Based on 16S rRNA Gene Analysis
https://doi.org/10.22146/jtbb.86256 Anita Nur Indahsari(1), Hari Purwanto(2*)
(1) Undergraduate Program, Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Bulaksumur, Sleman 55281, Yogyakarta, Indonesia.
(2) Laboratory of Entomology, Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Bulaksumur, Sleman 55281, Yogyakarta, Indonesia.
(*) Corresponding Author
Abstract
Honey is known for its various benefits for health, cosmetic ingredients, and other industrial materials. Especially, during the Covid-19 pandemic, many people consume honey to maintain body endurance. In Indonesia, the honey produced is dominated by Apis mellifera honey. With a cheaper price and a larger quantity, A. mellifera honey is often offered as forest honey or stingless bee honey to get more profit. Therefore, this study aims to determine the entomological origin of honey claimed as forest honey and stingless bee honey sold in the Indonesian market using the detection of 16S rRNA gene amplicon. This study tested 30 samples of forest honey and 30 samples of stingless bee honey. DNA that has been isolated from honey samples was amplified by PCR using 16S rRNA primers. The results from the sequence analysis showed that nine of honey samples were identified as honey fraud. Two samples were confirmed as falsification of the origin of honey-producing bees and four honey samples were confirmed as honey mislabelling. From this study it can be concluded that, it is possible to determine the entomological origin of honey molecularly by sequencing the 16S rRNA gene. Therefore, this method can be used to identify honey fraud that may occur on the market.
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APIMONDIA, 2019. Apimondia statement on honey fraud,
Badan Pusat Statistik, 2020. Statistik Produksi Kehutanan. Direktorat Statistik Peternakan, Perikanan, dan Kehutanan, p.xii + 68.
Buchori, D. et al., 2022. Beekeeping and managed bee diversity in Indonesia: perspective and preference of beekeepers. Diversity, 14(52), pp.1–14. doi: 10.3390/d14010052.
Byrne, S.J. et al., 2018. Taxonomy of oral bacteria 1st ed., Australia: Elsevier Ltd. doi: 10.1016/bs.mim.2018.07.001.
Engel, M.S., 2012. The honey bees of Indonesia (Hymenoptera: Apidae). Treubia, 39, pp.41–49.
FAO, 2019. Codex Alimentarius Standard for Honey,
Gratzer, K. et al., 2019. Challenges for beekeeping in Indonesia with autochthonous and introduced bees. Bee World, pp.1–5. doi: 10.1080/0005772x.2019.1571211.
Hall, R., 2009. Southeast Asia’s changing palaeogeography. Blumea: Journal of Plant Taxonomy and Plant Geography, 54, pp.148–161. doi: 10.3767/000651909X475941.
Hori, Y. & Engel, C., 2023. Regulation of ribosomal RNA gene copy number , transcription and nucleolus organization in eukaryotes. Nature reviews molecular cell biology, 24, pp.414–429. doi: 10.1038/s41580-022-00573-9.
Kadri, K., 2019. Polymerase chain reaction (PCR): principle and applications. In IntechOpen. London, pp. 1–17. Available at: https://www.intechopen.com/books/advanced-biometric-technologies/liveness-detection-in-biometrics.
Kahono, S., Chantawannakul, P. & Engel, M.S., 2018. Social bees and the current status of beekeeping in Indonesia. In Asian Beekeeping in the 21st Century. Springer Singapore, pp.287–306. doi: 10.1007/978-981-10-8222-1_13.
Kek, S.P. et al., 2017. Molecular identification of honey entomological origin based on bee mitochondrial 16S rRNA and COI gene sequences. Food Control, 78, pp.150-159. doi: 10.1016/j.foodcont.2017.02.025.
Kim, C.K., Lee, D.C. & Choi, S.H., 2017. Detection of Korean native honey and european honey by using duplex polymerase chain reaction and immunochromatographic assay. Korean Journal for Food Science of Animal Resources, 37(4), pp.599–605. doi: 10.5851/kosfa.2017.37.4.599.
Matsuzawa, T., Kohsaka, R. & Uchiyama, Y., 2020. Application of Environmental DNA: Honey Bee behavior and Ecosystems for Sustainable Beekeeping. Intech open, pp. 1–19.
Pribadi, A., 2016. Hutan tanaman industri jenis Eucalyptus sp. sebagai pakan. Info Teknis Eboni, 13(2), pp.105–118.
Raffiudin, R. et al., 2023. Entomological origin detection of honey from Apis mellifera and Apis cerana javana in Indonesia based on the Major Royal Jelly Protein 2 (mrjp2) gene. Journal of Apicultural Research, 62(2), pp.330–333. doi: 10.1080/00218839.2021.1989795.
Ribani, A. et al., 2020. Veterinary sciences honey as a source of environmental DNA for the detection and monitoring of honey bee pathogens and parasites. Veterinary Science, 7(113), pp.1–14.
Röder, B. et al., 2010. Impact of long-term storage on stability of standard DNA for nucleic acid-based methods. Journal of Clinical Microbiology, 48(11), pp.4260–4262. doi: 10.1128/JCM.01230-10.
Samarghandian, S., Farkhondeh, T. & Samini, F., 2017. Honey and health: a review of recent clinical research. Pharmacognosy Research, 9(2), pp.121–127. doi: 10.4103/0974-8490.204647.
Shao, W., Khin, S. & Kopp, W.C., 2012. Characterization of effect of repeated freeze and thaw cycles on stability of genomic DNA using pulsed field gel electrophoresis. Biopreservation and Biobanking, 10(1), pp.4–11. doi: 10.1089/bio.2011.0016.
Thummajitsakul, S. et al., 2013. The partial mitochondrial sequence of the Old World stingless bee, Tetragonula pagdeni. Journal of Genetics, 92(2), pp.299–303. doi: 10.1007/s12041-013-0243-1.
Zhang, Y.Z. et al., 2019. Authentication of Apis cerana honey and Apis mellifera honey based on major royal jelly protein 2 gene. Molecules, 24(289), pp.1–9. doi: 10.3390/molecules24020289.
DOI: https://doi.org/10.22146/jtbb.86256
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