* Corresponding Author
Genetic Variation of Butternut Squash (Cucurbita moschata Duchesne) based on Inter-Simple Sequence Repeat
https://doi.org/10.22146/jtbb.77228 Nabila Rizki Ananda Putri(1), Prima Sekti Kusnanda(2), Bunga Finovel Angellya(3), Dian Sartika(4), Purnomo Purnomo(5), Budi Setiadi Daryono(6*)
(1) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
(2) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
(3) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
(4) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
(5) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
(6) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
(*) Corresponding Author
Abstract
Butternut squash (Cucurbita moschata) is a Cucurbitaceae plant that has been widely cultivated in Indonesia. Butternut squash is known to have various cultivars. A new cultivar introduced by the Faculty of Biology UGM is named ‘Citra Laga’ which is expected to be able to compete with the imported cultivars. The number of cultivars within a species may indicate genetic variation. This research was conducted to observe genetic variation and the phenetic relationship between ‘Citra Laga’ and the imported butternut squash cultivars based on the molecular marker ISSR. The ISSR analysis between ‘Citra Laga’ and the imported cultivars showed an average low polymorphism rate by 18.61% with a high similarity percentage of 83.7%. Thus, it can be said that the genetic variation is low and ‘Citra Laga’ is not genetically much different from the imported cultivars.
Keywords
Full Text:
PDFReferences
Abdein, M.A.E-H., 2018. Genetic diversity between pumpkin accessions growing in the northern border region in Saudi Arabia based on biochemical and molecular parameters. Egyptian Journal of Botany, 58(3), pp.463-375. doi: 10.21608/EJBO.2018.3612.1171.
Andriyani & Jadid, N., 2021. Genetic diversity and morphological responses of Capsicum annuum varieties under alumunium stress. Biodiversitas, 22(5), pp.2576-2582. doi: 10.13057/biodiv/d220516.
Dari, L. & Yaro, N.S., 2016. Nutritional composition and storage of butternut squash. Ghana Journal of Horticulture, 12(1), pp.25-31.
Daryono, B.S. & Maryanto, S.D., 2017. Keanekaragaman dan Potensi Sumber Daya Genetik Melon, Yogyakarta: Gadjah Mada University Press.
Gupta, N., 2019. DNA extraction and polymerase chain reaction. Journal of Cytology, 36(2), pp.116-117. doi: 10.4103/JOC.JOC_110_18.
Hidzroh, F. & Daryono, B.S., 2021. Keseragaman dan kestabilan karakter tanaman melon (Cucumis melo L. ‘Tacapa Gold’) berdasarkan karakter fenotip dan Inter-Simple Sequence Repeat. Biospecies, 14(2), pp.11-19.
Inan, N. et al., 2012. Efficacy of ISSR and SRAP techniques for molecular characterization of some Cucurbita genotypes including naked (hull-less) seed pumpkin. The Journal of Animal and Plant Sciences, 22(1), pp.126-136.
Marie-Magdeleine, C., Mahie, M., & Archimede, H., 2011. Pumpkin (Cucurbita moschata Duchesne ex Poir.) Seeds as an Anthelmintic Agent?. In Nuts and Seeds in Health and Disease Prevention. Massachusetts: Academic Press, pp.933-939.
Melani, R., Adiartayasa, W., & Wijaya, I.N., 2018. Deteksi penyakit Citrus Vein Phloem Degenation (CVPD) dengan teknik Polymerase Chain Reaction (PCR) pada daun tanaman jeruk yang memiliki pola gejala kronis berbeda. E-Jurnal Agroteknologi Tropika, 7(2), pp.104-173.
Munif, A. et al., 2004. Polimorfisme genetik dari Anopheles barbirostris kaitannya dengan prevalensi malaria di Kecamatan Cineam, Kabupaten Tasikmalaya. Buletin Penelitian Kesehatan, 32(1), pp.1-16.
Ng, W.L., & Tan, S.G., 2015. Inter-simple sequence repeat (ISSR) markers: are we doing it right?. ASM Science Journal, 9(1), pp.30-39.
Nopianasanti, H. & Daryono, B.S., 2018. Kestabilan fenotip tanaman labu susu (Cucurbita moshata (Duchesne) Poir "Butternut") hasil budidaya di Sleman D.I Yogyakarta. Biogenesis, 6(2), pp.115-123. doi: 10.24252/bio.v6i2.4751.
Purnomo, Daryono, B.S., & Sentori, M.B., 2015. Variability and intraspecies classification of pumpkin (Cucurbita moschata (Duch. ex Lam.) Duch. ex Poir.) based on morphological characters. KnE Life Sciences, 2, pp.286-293. doi: 10.18502/kls.v2i1.160.
Rahayu, D.A. & Jannah, M., 2019. DNA Barcode Hewan dan Tumbuhan Indonesia, Jakarta: Yayasan Inspirasi Ide Berdaya.
Samiyarsih, S. et al., 2020. Anatomical profile and genetic variability of sweet potato (Ipomoea batatas) cultivars in Banyumas, Central Java, based on RAPD markers. Biodiversitas, 21(4), pp.1755-1766. doi: 10.13057/biodiv/d210460.
Sari, S.K. et al., 2014. Optimasi teknik isolasi dan purifikasi dna pada daun cabai rawit (Capsicum frutescens cv. Cakra Hijau) menggunakan genomic DNA mini kit (plant) Geneaid. Prosiding Seminar Nasional XI Pendidikan Biologi FKIP UNS, 11(1), pp.65-70.
DOI: https://doi.org/10.22146/jtbb.77228
Article Metrics
Abstract views : 1561
| 
views : 1763
Refbacks
- There are currently no refbacks.
Copyright (c) 2023 Journal of Tropical Biodiversity and Biotechnology
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Editoral address:
Faculty of Biology, UGM
Jl. Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia
ISSN: 2540-9581 (online)