* Corresponding Author
Anti-hypercholesterolemia, Anti-atherogenic, and Anti-hypertension Effects of Red Beetroot (Beta vulgaris L.) in Rats Induced by High Fat and Fructose Diet
https://doi.org/10.22146/jtbb.75914 Alim El-Hakim(1), Sunarti Sunarti(2), Lisna Hidayati(3), Slamet Widiyanto(4*)
(1) Undergraduate Student Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(2) Department of Biochemistry, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(3) Laboratory of Biochemistry, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(4) Laboratory of Animal Physiology, Faculty of Biology, Universitas Gadjah Mada, Yogyakarta, Indonesia 55281
(*) Corresponding Author
Abstract
Metabolic syndrome is associated with abnormalities of lipid levels in the blood such as hyperlipidemia. Hyperlipidemia conditions can increase the risk of atherosclerosis and hypertension. Beetroot (Beta vulgaris L.) is a plant that contains high antioxidants. Beetroot has the potency to be used as a functional food that can reduce the potential for atherosclerosis and blood pressure. The aim of this study was to examine the effect of beetroot-enriched feed on the cholesterol level, atherogenic index and blood pressure of rats (Rattus norvegicus Berkenhout, 1769) induced by high fat and fructose diet. As many as 25 rats were divided into control, hyperlipidemia, and three treatment groups. The hyperlipidemia and treatment groups were induced to become hyperlipidemia using AIN93-M modified high fat and fructose feed for 8 weeks. The treatment groups were followed by intervention with 6, 9, and 12% beetroot enriched feed for 6 weeks. The feed was prepared by mixing beetroot flour in the pellets. Blood pressure, total cholesterol, and HDL level measurement was conducted after hyperlipidemia induction and after the intervention. The data analyzed with one-way ANOVA, DMRT, and T-Test. The results showed that 6% beetroot intervention have the highest increasing of HDL-cholesterol than other groups. The 9% beetroot intervention significantly decrease total cholesterol lower than normal baseline, and 12% beetroot intervention significantly decrease blood pressure than other groups. The atherogenic index of all treatment group was decreased. The 9% beetroot enriched feed was seen as an optimum dose to reduce total cholesterol, atherogenic index and blood pressure and increase HDL-cholesterol.
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Al-Dosari, M.S. et al., 2011. Effect of Beta vulgaris L. on cholesterol rich diet-induced hypercholesterolemia in rats. Farmacia, 59(5), pp.669-677.
Aviram, M. et al., 2004. Pomegranate juice consumption for 3 years by patients with carotid artery stenosis reduces common carotid intima-media thickness, blood pressure and LDL oxidation. Clinical nutrition, 23(3), pp.423–433. doi: 10.1016/j.clnu.2003.10.002
Ayala, A., Muñoz, M.F. & Argüelles, S., 2014. Lipid Peroxidation: Production, Metabolism, and Signaling Mechanisms of Malondialdehyde and 4-Hydroxy-2-Nonenal. Oxidative Medicine and Cellular Longevity, 2014, 360438. doi: 10.1155/2014/360438
Ceclu, L. & Nistor, O., 2020. Red beetroot: Composition and health effects - A review. Journal of Nutritional Medicine and Diet Care, 6(043). doi: 10.23937/2572-3278.1510043
Clifford, T. et al., 2015. The Potential Benefits of Red Beetroot Supplementation in Health and Disease. Nutrients, 7(4), pp.2801–2822. doi: 10.3390/nu7042801
Chen, L. et al., 2021. Beetroot as a functional food with huge health benefits: Antioxidant, antitumor, physical function, and chronic metabolomics activity. Food science & nutrition, 9(11), pp.6406–6420. doi: 10.1002/fsn3.2577
Fardiaz, S., 2014. Mikrobiologi Pangan I. Universitas Terbuka
Gokhale, S.V. & Lele, S.S., 2014. Betalain Content and Antioxidant Activity of Beta vulgaris: Effect of Hot Air Convective Drying and Storage. Journal of Food Processing and Preservation, 38(1), pp.585–590. doi: 10.1111/jfpp.12006
Goldman, I.L. & Navazio, J.P., 2007. Table Beet. In Handbook of Plant Breeding. NY: Springer, pp.219–238. doi: 10.1007/978-0-387-30443-4_7
Hannou, S.A. et al., 2018. Fructose metabolism and metabolic disease. The Journal of clinical investigation, 128(2), pp.545–555. doi: 10.1172/JCI96702.
John, R. & Singla, A., 2021. Functional Foods: Components, health benefits, challenges, and major projects. DRC Sustainable Future: Journal of Environment, Agriculture, and Energy, 2(1), pp.61-72. doi: 10.37281/drcsf/2.1.7
Kazemi, T. et al., 2018. Cardiovascular Risk Factors and Atherogenic Indices in an Iranian Population: Birjand East of Iran. Clinical Medicine Insights: Cardiology, 20(12), 1179546818759286. doi: 10.1177/1179546818759286
Lee, J. H., Ozcelik, B. & Min, D. B., 2003. Electron donation mechanisms of β-carotene as a free radical scavenger. Journal of Food Science, 68(3), pp.861–865. doi: 10.1111/j.1365-2621.2003.tb08256.x
Lingling, C. et al., 2018. Preparation of Potato Whole Flour and Its Effects on Quality of Flour Products: A Review. Grain & Oil Science and Technology, 1(3), pp.145–150. doi: 10.3724/sp.j.1447.gost.2018.18037
Lozano, I. et al., 2016. High-fructose and high-fat diet-induced disorders in rats: impact on diabetes risk, hepatic and vascular complications. Nutrition & Metabolism, 13, 15. doi: 10.1186/s12986-016-0074-1
Lu, Y. et al., 2015. Lifestyle and Risk of Hypertension: Follow-Up of a Young Pre-Hypertensive Cohort. International Journal of Medical Sciences, 12(7), pp.605–612. doi:10.7150/ijms.12446
Murphy, A.J. et al., 2012. Anti-atherogenic mechanisms of high-density lipoprotein: Effects on myeloid cells. Biochimica et Biophysica Acta (BBA) - Molecular and Cell Biology of Lipids, 1821(3), pp.513–521. doi: 10.1016/j.bbalip.2011.08.003
Nahla, T.K., Wisam, S.U. & Tariq, N.M., 2018. Antioxidant Activities of Beetroot (Beta vulgaris L.) Extracts. Pakistan Journal of Nutrition: PJN, 17(10), pp.500–505. doi: 10.3923/pjn.2018.500.505
Nelson, R.H., 2013. Hyperlipidemia as a Risk Factor for Cardiovascular Disease. Primary Care: Clinics in Office Practice, 40(1), pp.195–211. doi: 10.1016/j.pop.2012.11.003
Othman, Z.A. et al., 2019. Phenolic Compounds and the Anti-Atherogenic Effect of Bee Bread in High-Fat Diet-Induced Obese Rats. Antioxidants, 9(1), p.33. doi:10.3390/antiox9010033
Pan, J. et al., 2018. Prevalence of hyperlipidemia in Shanxi Province, China and application of Bayesian networks to analyse its related factors. Scientific Reports, 8, 3750. doi: 10.1038/s41598-018-22167-2
Parik, T. et al., 1996. Evidence for Oxidative Stress in Essential Hypertension: Perspective for Antioxidant Therapy. European Journal of Cardiovascular Prevention & Rehabilitation, 3(1), pp.49–54. doi: 10.1177/174182679600300107
Purnell, J.Q., 2018. Definitions, Classification, and Epidemiology of Obesity. In Endotext (Internet). South Dartmouth (MA): MDText.com, Inc.
Riccioni, G. et al., 2009. Relationship between plasma antioxidant concentrations and carotid intima-media thickness: the Asymptomatic Carotid Atherosclerotic Disease in Manfredonia Study. European Journal of Cardiovascular Prevention & Rehabilitation, 16(3), pp.351–357. doi:10.1097/HJR.0b013e328325d807
Rochlani, Y. et al., 2017. Metabolic syndrome: pathophysiology, management, and modulation by natural compounds. Therapeutic Advances in Cardiovascular Disease, 11(8), pp.215–225. doi: 10.1177/1753944717711379
Santos-Sánchez, N.F. et al., 2019. Antioxidant compounds and their antioxidant mechanism. In Antioxidants. IntechOpen. doi: 10.5772/intechopen.85270
Scuteri, A. et al., 2005. The Metabolic Syndrome in Older Individuals: Prevalence and Prediction of Cardiovascular Events: The Cardiovascular Health Study. Diabetes Care, 28(4), pp.882–887. doi:10.2337/diacare.28.4.882
Singh, A. et al., 2015. Beetroot Juice Supplementation Increases High Density Lipoprotein-Cholesterol and Reduces Oxidative Stress in Physically Active Individuals. Journal of Pharmacy and Nutrition Sciences, 5(3), pp.179–185. doi: 10.6000/1927-5951.2015.05.03.2
Sorriento, D. et al., 2018. The Antioxidant Therapy: New Insights in the Treatment of Hypertension. Frontiers in Physiology, 9, 258. doi: 10.3389/fphys.2018.00258
Stanhope, K.L., 2009. Consuming fructose-sweetened, not glucose-sweetened, beverages increases visceral adiposity and lipids and decreases insulin sensitivity in overweight/obese humans. The Journal of clinical investigation, 119(5), pp.1322–1334. doi: 10.1172/JCI37385
Suanarunsawat, T. et al., 2011. Lipid-Lowering and Antioxidative Activities of Aqueous Extracts of Ocimum sanctum L. Leaves in Rats Fed with a High-Cholesterol Diet. Oxidative Medicine and Cellular Longevity, 2011, 962025. doi: 10.1155/2011/962025.
Tedla, Y.G., & Bautista, L.E., 2016. Drug Side Effect Symptoms and Adherence to Antihypertensive Medication. American journal of hypertension, 29(6), pp.772–779. doi: 10.1093/ajh/hpv185
Wang, H.H. et al., 2017. Cholesterol and Lipoprotein Metabolism and Atherosclerosis: Recent Advances in Reverse Cholesterol Transport. Annals of Hepatology, 16, pp.S27–S42. doi: 10.5604/01.3001.0010.5495
World Health Organization, 2021, ‘Obesity and overweight’, in World Health Organization, viewed from https://www.who.int/news-room/fact-sheets/detail/obesity-and-overweight.
You, C.L., Su, C.L. & Zhou, C.L., 2008. Study on Effect and mechanisms of Scutellaria baicalensis stem-leaf total flavonoid in regulating lipid metabolism. China J. Chin. Mater. Med., 33(9), pp.1064–1066.
DOI: https://doi.org/10.22146/jtbb.75914
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