The Effectiveness of Red Spinach (Amaranthus tricolor L.) and Green Spinach (Amaranthus hybridus L.) Extracts for Bacillus thuringiensis var. kurstaki Protectant against UVB Radiation for the Control of Armyworm (Spodoptera litura Fab.)

https://doi.org/10.22146/jtbb.53004

Siti Sumarmi(1), Mifta Arlinda(2), Sukirno Sukirno(3*)

(1) Faculty of Biology, Gadjah Mada University
(2) Faculty of Biology, Gadjah Mada University
(3) Faculty of Biology, Gadjah Mada University
(*) Corresponding Author

Abstract


Spodoptera litura Fab. is an insect that damage cultivated plants in Indonesia. Efforts to control it can be done by using biological agents for example by using Bacillus thuringiensis kurstaki (Btk.). Unfortunately, the Btk. is easily degraded by UV radiation. This research aimed to study the effectiveness of red and green spinach as UVB protection for Btk. and to observe the pathogenicity of Btk. formulations against armyworm. Furthermore, the sublethal effect of Btk. against S. litura was investigated. The morphology of the endospore, protein crystal, and bacterium were observed under a contrast phase microscope. The extracts at 2% (w/v) were mixed with Btk. suspensions at 5 x 104, 5x105, and 5x106 (spores/ml), respectively. The formulations then exposed under Ultraviolet B (UVB) lights for 3, 6, and 9 hours then tested against the 3rd larval instar of armyworm. The larval mortality was observed daily and the analysis of variance was analyzed by one way anova. The sublethal effects of the treatment to the pupal and adult stages were observed when the moths emerge. The results showed that the larval mortality caused by  Btk. mixed with red spinach ranged from 11.7 to 26.7%. The sublethal effects of Btk. resulted in smaller sizes of pupae and imago, darker pupae, and wings abnormality of the adult stage, compared to any control treatment. The morphological observation of the bacteria showed that extracts gave UV protection against UVB. These results suggested that red and green spinach potentially can be used as a protectant for Btk. against UVB.


Keywords


Bacillus thuringiensis var. kurstaki (Btk.); Pathogenicity; Spinach; Spodoptera litura; UVB

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References

Amin, I., Norazaidah, Y. & Hainida, K.E., 2006, Antioxidant activity and phenolic content of raw and blanched Amaranthus species. Food Chemistry 94(1), 47-52.

Anbhudasan, P., Surendraraj, A., Karkhuzali, S. & Sathiskumara, S., 2014, Natural antioxidant and its benefit. International journal of food and nutrition sciences 3(6), 225-232.

Arlinda, M., 2019, The effectiveness of red spinach (Amaranthus tricolor L.) and green spinach (Amaranthus viridis L.) as UV B radiation protection for Bacillus thuringiensis var. kurstaki controller Spodoptera litura Fab., [Efektivitas bayam merah (Amaranthus tricolor L.) dan bayam hijau (Amaranthus viridis L.) Sebagai protektan radiasi UV B bagi Bacillus thuringiensis var. kurstaki pengendali Spodoptera litura Fab.], Undergraduate thesis. Faculty of Biology. Universitas Gadjah Mada. Yogyakarta.

Bouda. H., Tapondjou, L.A., Fontem, D.A. & Gumedzoe Y.D., 2001, Effect of essential oils from leaves of Ageratum conyzoides, Lantara camara and Chromolaena odorata on the mortality of Sitophylus zeamays (Coleoptera, Curculionidae). Journal of Stored Products Research 37, 103-109.

Cagan, L. & Barta, M., 2008, Sublethal effect of Bt-maize in semi-artificial diet on european corn borer larvae, Ostrinia nubilalis (Hubner, 1796) (Lepidoptera, Crambidae). Insect Pathogens and Insect Parasitic Nematodes IOBC Bulletin 31, 127-130.

El-Sharkawey, A.Z., Ragaei, M., Sabbour, M.M., Afaf, A.A., Mohamed, H.A.L.A. & Samy, R., 2009, Laboratory evaluation of antioxidants as UV-protectants for Bacillus thuringiensis against potato tuber moth larvae. Australian Journal of Basic and Applied Sciences 3(2), 358-370.

Feitelson, J.S., Payne, J. & Kim, L., 1992, Bacillus thuringiensis: Insect and Beyond. Biotechnology 10, 271-275.

Finney, D.J., 1949, The adjustment for a natural response rate in probit analysis. Annals of Applied Biology 36(2), 187-195.

Griego, V.M. & Spence, K.D., 1978, Inactivation of Bacillus thuringiensis spore by ultraviolet and visible light. Applied Environmental Microbiology 35, 906-910.

Khasdan, V., Ben‐Dov, E., Manasherob, R., Boussiba, S., & Zaritsky, A., 2001, Toxicity and synergism in transgenic Escherichia coli expressing four genes from Bacillus thuringiensis subsp. israelensis. Environmental microbiology 3(12), 798-806.

Khetan, S.K., 2001, Microbial pest control. Maecell Dekker, Inc. USA. p: 141

Marwoto dan K.E. Neering, 1992, Soybean pest control with insecticides-based monitoring, [Pengendalian hama kedelai dengan insektisida berdasarkan pemantauan]. Risalah Lokakarya Pengendalian Hama Terpadu Tanaman Kedelai. Balai Penelitian Tanaman Pangan Malang, 59−65.

Ningrum, A.D. 2019. Tea leaf extract (Camellia sinensis (L.) Kuntze) as a protectant Bacillus thuringiensis var. kurstaki against ultraviolet light for control of armyworm (Spodoptera litura Fab.) [Ekstrak daun teh (Camellia sinensis (L.) Kuntze) sebagai protektan Bacillus thuringiensis var. kurstaki terhadap sinar ultraviolet untuk pengendalian ulat grayak (Spodoptera litura Fab.)]. Undergraduate thesis. Faculty of Biology Universitas Gadjah Mada. Yogyakarta.

Schnepf, E., Crickmore, N., Van Rie, J., Lereclus, D., Baum, J., & Feitelson, J., Zeigler, D.R. and Dean, D., 1998, Bacillus thuringiensis and its pesticidal crystal protein. Microbiology and Molecular Biology Reviews 62 (3): 775-806.

Schunemann R., Knaak, N., & Fluza, L.M., 2014, Mode of action and specifity of Bacillus thuringiensis toxin in the control of caterpillars and stink bugs in soybean culture. ISRN Microbiology 2014, 1-12.

Shorey, H.H. & Hale, R.L., 1965, Mass-rearing of larvae of nine noctuid species on simple artificial medium. Journal of Economic Entomology 58: 522-524.

Sukirno, S., Priana, A.M., & Nayula, R.E.S., 2017, Effectiveness of dragon fruit extracts as UV protectant Bacillus thuringiensis cabbage caterpillar pest controller (Crocidolomia binotalis), [Efektifitas ekstrak buah naga sebagai UV protektan Bacillus thuringiensis pengendali hama ulat kobis (Crocidolomia binotalis)]. Scientific reports of students and lecturer collaborative research. Faculty of Biology Universitas Gadjah Mada. Yogyakarta.

Sukirno, S., Tufail, M., Rasool, K.G., El Salamouny, S., Sutanto, K.D., & Aldawood, A.S., 2018, The efficacy and persistence of Spodoptera littoralis nucleopolyhedrovirus (SpliMNPV) applied in UV protectants against the beet armyworm, Spodoptera exigua (Hübner)(Lepidoptera: Noctuidae) under Saudi field conditions. Pakistan Journal of Zoology 50(5), 1895-1902.

Sukirno, S., Tufail, M., Rasool, K.G., El Salamouny, S., Sutanto, K.D., & Aldawood, A.S., 2017, The effectiveness of spinosad and neem extract against Spodoptera littoralis (Boisd.) and Spodoptera exigua (Hubner): Exploring possibilities to enhance the bio-pesticide persistence with natural UV protectants under field-sunlight conditions of Saudi Arabia. Pakistan Journal of Agricultural Sciences 54(4), 743-751.

Sutanto, K. D., El Salamouny, S., Tufail, M., Ghulam Rasool, K., Sukirno, S., Shepard, M., Shapiro, M., & Aldawood, A.S.A., 2017, Evaluation of natural additives to enhance the persistence of Spodoptera littoralis (Lepidoptera: Noctuidae) nucleopolyhedrovirus (SpliMNPV) under field conditions in Saudi Arabia. Journal of Economic Entomology 110(3), 924-930.

Tarigan, A.B. 2019. The effectiveness of aloe (Aloe vera L.) as Bacillus thuringiensis var kurstaki protectant against ultraviolet rays and insect biological control agent armyworm (Spodoptera litura Fab.), [Efektivitas lidah buaya (Aloe vera L.) sebagai protektan Bacillus thuringiensis var kurstaki terhadap sinar ultraviolet dan agen pengendali hayati serangga ulat grayak (Spodoptera litura Fab.)], Undergraduate thesis. Faculty of Biology, Universitas Gadjah Mada. Yogyakarta.

USDA, 2020, Cabbage, raw nutrition facts and analysis per serving. https://www.nutritionvalue.org/Cabbage%2C_raw_nutritional_value.html. Accessed on June 29th, 2020.



DOI: https://doi.org/10.22146/jtbb.53004

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