* Corresponding Author
Genetic Identification of Freshwater Fish Species Through DNA Barcoding from Lake Lebo Taliwang, West Nusa Tenggara
https://doi.org/10.22146/jtbb.46256 Tuty Arisuryanti(1*), Rika Lathif Hasan(2), Khadija Lung Ayu(3), Nofita Ratman(4), Lukman Hakim(5)
(1) Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada
(2) Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada
(3) Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada
(4) Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada
(5) Laboratory of Genetics and Breeding, Faculty of Biology, Universitas Gadjah Mada
(*) Corresponding Author
Abstract
Lake Lebo Taliwang is one of the lakes in the West Nusa Tenggara with high freshwater fish species diversity. However, the species identification of freshwater fish species from Lake Lebo Taliwang using DNA barcoding is very limited. Therefore, the objective of this study was to identify seven samples of freshwater fish species collected from Lake Lebo Taliwang based on COI mitochondrial gene as a DNA barcoding marker and establish library COI sequences of Indonesian freshwater fish. We are using a standard DNA analysis and data obtained from this study was then examined using Nucleotide BLAST and the phylogenetic tree was analyzed using the Neighbour-Joining (NJ) method with Kimura 2 Parameter (K2P) model. The results revealed that among the seven samples of freshwater fishes collected from the Lake Lebo Taliwang, three samples were identified as Anabas testudineus, the other three samples were Barbodes binotatus and one sample was Trichopodus trichopterus. The level of similarity of these freshwater fish samples referred to the database from the GenBank and BOLD was between 98-100%. The NJ tree supports the clade of each species identified in this study. This occurrence indicated that DNA barcoding by using the COI mitochondrial gene was proven to be able to identify the freshwater fish samples accurately.
Keywords
Full Text:
PDFReferences
Arif, I.A. & Khan, H.A., 2009, Molecular markers for biodiversity analysis of wild animals: a brief review, Animal Biodiversity and Conservation, 32(1), 9-17.
Arisuryanti, T., Hasan, R.L. & Koentjana, J.P., 2018, Genetic identification of two mudskipper species (Pisces: Gobiidae) from Bogowonto Lagoon (Yogyakarta, Indonesia) using COI mitochondrial gene as a DNA barcoding marker, AIP Conference Proceedings, 2002, 020068(1)-020068(7).
Chauhan, T. & Rajiv, K., 2010, Molecular markers and their applications in fisheries and aquaculture, Advances in Bioscience and Biotechnology, 01(04), 281-291.
Dahruddin, H., Hutama, A., Busson, F., Sauri, S., Hanner, R., Keith, P., Hadiaty, R., & Hubert, N., 2017, Revisiting the ichthyodiversity of Java and Bali through DNA barcodes: taxonomic coverage, identification accuracy, cryptic diversity and identification of exotic species, Molecular Ecology Resources, 17(2), 288-299.
Dudu, A., Barbălată, T., Popa, G., Georgescu, S.E., Costache, M., 2016, Advantages and limitations of DNA Barcoding in identifying commercially-exploited fish species, Animal Science and Biotechnologies, 49 (1), 45-49.
Fricke, R., Eschmeyer, W. N. & Van der Laan, R., 2019, Eschmeyer's catalog of fishes: genera, species, references, viewed 1 June 2019, http://researcharchive.calacademy.org/research/ichthyology/catalog/fishcatmain.asp.
Haryani, G., 2013, Kondisi danau di Indonesia dan strategi pengselolaannya. Prosiding Pertemuan Ilmiah Tahunan Masyarakat Limnologi Indonesia I, 1-19.
Hebert, P.D., Ratnasingham, S. & deWaard, J.R., 2003, Barcoding animal life: cytochrome c oxidase subunit 1 divergence among closely related species, Proceedings of the Royal Society B: Biological Sciences, 270 Suppl 1, S96-99.
Hutama, A., Dahruddin, H., Busson, F., Sauri, S., Keith, P., Hadiaty, R.K., Hanner, R., Suryobroto, B. & Hubert, N., 2017, Identifying spatial concordant evolutionary significant units across multiple species through DNA barcodes: Application to the conservation genetics of the freshwater fishes of Java and Bali, Global Ecology and Conservation, 12, 170-187.
Kumar, S., Stecher, G., & Tamura, K., 2016, MEGA7: molecular evolutionary genetic analysis version 7.0 for bigger datasets, Molecular Biology and Evolution, 33(7), 1870-1874.
Librado, P., & Rozas, J., 2009, DnaSP v5: a software for comprehensive analysis of DNA polymorphism data. Bioinformatics, 25(11), 1451-1452.
Maddison, W.P., & Maddison, D.R., 2018, Mesquite: A modular system for evolutionary analysis. Version 3.51 http://www.mesquiteproject.org.
Peninal, S., Subramanian, J., Elavarasi, A. & Kalaiselvam, M., 2017, Genetic identification of marine eels through DNA barcoding from Parangipettai coastal waters. Genomics Data, 11, 81-84.
Pettengill, J.B. & Maile, C.N., 2010, An evaluation of candidate plant DNA barcodes and assignment methods in diagnosing 29 species in the genus Agalinis (Orobanchaceae). American Journal of Botany, 97,1391–1406.
Ratnasingham, S. & Hebert, P.D.N., 2007, BOLD: The barcode of life data system (www.barcodinglife.org). Molecular Ecology Notes, 7, 355-364.
RIPED, 2008, Lebo Taliwang, kekayaan flora dan fauna Sumbawa Barat, viewed 27 February 2019, http://konservasi4lebotaliwang.blogspot.com
Syaifudin, M., Jubaedah, D., Muslim, M. & Daryani, A., 2017, DNA authentication of Asian Red Catfish Hemibragus nemurus from Musi and Penukal River, South Sumatra Indonesia. Genetics of Aquatic Organisms, 1, 43-48.
Yang, L., Tan, Z.,Wang, D., Xue, L., Guan, M-X., Huang, T. & Li, R., 2014, Species identification through Mitochondrial rRNA genetic analysis. Scientific Reports, 4:4089.
Yudhistira, A & Arisuryanti, T., 2019, Preliminary findings of cryptic diversity of the giant tiger shrimp (Penaeus monodon Fabricius, 1798) in Indonesia inferred from COI mitochondrial DNA. Genetika, 51, 261-270.
Ward, R.D., Zemlak, T.S., Innes, B.H., Last, P.R., & Hebert, P.D.N., 2005, DNA barcoding Australia's fish species. Philosophical Transactions of the Royal Society B, 360(1462), 1847-1857.
DOI: https://doi.org/10.22146/jtbb.46256
Article Metrics
Abstract views : 7799
| 
views : 5514
Refbacks
- There are currently no refbacks.
Copyright (c) 2019 Journal of Tropical Biodiversity and Biotechnology
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Editoral address:
Faculty of Biology, UGM
Jl. Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia
ISSN: 2540-9581 (online)