* Corresponding Author
Peptone and tomato extract induced early stage of embryo development of Dendrobium phalaenopsis Orchid
https://doi.org/10.22146/jtbb.15498 Nintya Setiari(1), Aziz Purwantoro(2), Sukarti Moeljopawiro(3), Endang Semiarti(4*)
(1) Faculty of Biology Universitas Gadjah Mada
(2) Faculty of Biology Universitas Gadjah Mada
(3) Faculty of Biology Universitas Gadjah Mada
(4) Faculty of Biology Universitas Gadjah Mada
(*) Corresponding Author
Abstract
Germination and growth of orchid seeds can be accelerated by the addition of organic supplement and plant extract in culture medium. The objective of this study was to determine the effect of peptone and tomato extract on early stage of embryo development of Dendrobium phalaenopsis orchids. Orchid seeds were sown on NP and VW medium with addition of 10% of CW (NPCW and VWCW). Five weeks after seed germination, about 58.03% seed germination was observed on VWCW medium, and only 37.45% seed germination on NPCW. Tomato extract and peptone were added in VWCW, resulting VWCWTP medium. After 4-8 weeks on VWCWTP, 94.42% seeds was germinated into plantlet, but only 67.30% germinated seeds on VWCW. To get optimal growth and development of D. phalaenopsis orchids embryos in the in vitro condition, supplement of 100 ml.L-1 coconut water, 100 mg.L-1 tomato extract and 2 mg.L-1 peptone into VW basic medium is required.
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Arditti J (1967) Factors affecting the germination of orchid seeds. Botanical Review. 33 (1): 1-97. Arditti, J (2008) Micropropagation of orchid, volume 1. Blackwell Publishing, USA. Butcher D & Marlow SA (1986) Asymbiotic germination of epiphytic and terrestrial orchid in Modern Methods in Orchid Conservation. In: The role of Physiology, Ecology and Management, Pritchard HW (Eds), Cambridge University Press Sydney. Charles and Baker M (1996) Dendrobium Species Culture. Orchids 65(11):1190-1195. Da Silva JAT (2013) Ammonium to nitrate ratio affects protocorm like bodies PLB formation in vitro of hybrid Cymbidium. Journal of Ornamental Plant (Journal of Ornamental and Horticultural Plant), 3 (3): 155-160. Davidson B (1993) Cultural requirements of Dendrobium-Phalaenopsis, growing orchids, a culture handbook, American Orchid Society. George EF & Sherrington PD (1984) Plant propagation by tissue culture. Exegetics Limited. Hossain MM (2008) Asymbiotic seed germination and in vitro seedling development of Epidendrum ibaguense Kunth. (Orchidaceae). African Journal of Biotechnology Vol. 7 (20): 3614-3619. Islam MO., Ichihasi S & Matsui S (1998) Control of growth and development of protocorm like body derived from callus by carbon source in Phalaenopsis. Plant Biotechnology. 15(4):183-187. Mondal S, Ahirwar MK, Singh MK, and Singh RP (2015) Effect of coconut water and ascorbic acid on shoot regeneration in banana variety dwarf Cavendish. Int. J. Bio-res. Env. Agril. Sci. 1(1): 65-69. Roy J & Banerjee N (2001) Cultural requirement for in vitro seed germination, protocorm growth and seedling development of Geodorum densiflorum (Lam.) Schltr. Indian Journal of Experimental Biology. 39:1041-1047. Semiarti E, Indrianto A, Purwantoro A, Martiwi INA, Feroniasanti YML, Nadifah F, Mercuriani IS, Iwakawa H, YoshiokaY, Machida Y & Machida C (2010) High-frequency genetic transformation of Phalaenopsis amabilis orchid using tomato extract-enriched medium for the pre-culture of protocorms. Journal of Horticultural Science and Biotechnology. 85(3):205-210. Rahardi F (2006) Kiat Budidaya Anggrek Spesies, dalam Selamatkan Anggrek Spesies Indonesia, Jakarta Orchid Festival 2006. Perhimpunan Anggrek Indonesia. Jakarta. Semiarti E, Indrianto A, Purwantoro A, Machida Y, Machida C (2011) Agrobacterium-mediated transformation of Indonesian orchid for micropropagation. http://cdn.intechopen.com/pdfs/18821/InTechAgrobacterium_mediated_transformation_of_indonesian_orchids_for_micropropagation.pdf. Shekarriz P, Kafi M, Deilamy SD, Mirmasoumi M (2014) Coconut water and peptonee improve seed germination and protocorm like body formation of hybrid Phalaenopsis, Agric. sci. dev. 3(10) : 317-322. Soad, Ibrahim MM, Taha LS and Farahat MM (2010) Influence of Foliar Application of Peptone on Growth, Flowering and Chemical Composition of Helichrysum bracteatum Plants under Different Irrigation Intervals. Ozean Journal of Applied Sciences 3(1). Suryowinoto M (1996) Pemuliaan Tanaman Secara in Vitro, Kanisius, Yogyakarta, Indonesia.
DOI: https://doi.org/10.22146/jtbb.15498
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