* Corresponding Author
Cloning of the gene encoding Endo-β-1,3-1,4 Glucanase from Bacillus subtilis subsp. spizizenii W23 on Plasmid pMMB67EH into Escherichia coli DH5α and Escherichia coli Origami
https://doi.org/10.22146/jsv.8114 Lisa Gunawan(1*)
(1) Fakultas Teknobiologi, Universitas Surabaya, Surabaya
(*) Corresponding Author
Abstract
One of the genes encoding cellulolytic enzymes, named BSUW23_10175, from Bacillus subtilis subsp. spizizenii W23 was cloned in the plasmid pMMB67EH into Escherichia coli DH5α and Escherichia coli Origami. Target gene was amplified using polymerase chain reaction (PCR) with specific primers (For- BSUW23_10175 dan Rev-BSUW23_10175) that were used for BSUW23_10175 gene amplification. Based on the PCR and restriction analyses of the transformants plasmid, it was known that clones number 8 and 9 of the Escherichia coli Origami transformants contained the recombinant plasmid pMMB_BSUW23_10175.
Cellulolytic activity assay showed that those transformants had cellulolytic activity by indution with IPTG (isopropyl-β-D-thio-galactosidase).
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Daftar Pustaka
Amore, Antonella, Olimpia Pepe, Valeria
Ventorino, Leila Birolo, Chiara Giangrande
and Vincenza Faraco FEMS Microbiol Lett.
Industrial waste based compost as a
source of novel cellulolytic strains and
e n z y m e s . D i a k s e s d a r i
http://pubget.com/paper/23181595/Industrial_
Kloning Gen Pengkode Endo-β-1,3-1,4 Glukanase Bacillus subtilis subsp.
Lisa Gunawan
waste_based_ compost_as_a_source_of_
novel_cellulolytic_strains_and_enzymes.
Diakses pada 11 Januari 2013.
DIBY, Paul, Kanamparambil Augusthy SAJU,
Pulikottil John JISHA, Yammanuru Ramalinga
SARMA, Aundy KUMAR and Muthuswamy
ANANDARAJ. 2005. Mycolytic enzymes
produced by Pseudomonas fluorescens dan
Trichoderma spp. Against Phytophthora
capsici, the foot rot pathogen of black pepper
( P i p e r n i g r u m L . ) . D i a k s e s d a r i
http://www.annmicro.unimi.it/full/55/diby_55
_129-133.pdf. Diakses pada 6 Mei 2012.
Chander, Ramesh Kuhad, Rishi Gupta, and Ajay
Singh. 2011. Microbial Cellulases and Their
Industrial Applications. Enzyme Research
Volume 2011 (2011), Article ID 280696.
Fruste, J. P., W. Pansegrau, R. Frank, H. Blöcker, P.
Scholz, M. Bagdasarian and E. Lanka 1986.
Molecular Cloning of the Plasmid RP4 Primase
Region in a Multi-host range tacP Expression
Vector. Gene 48, 119 – 131.
Gram, Hans Christian. 1884. The Differential
Staining of Schizomycetes in Tissue Sections
and in Dried Preparations. Fortschitte der
Medicin. 2:185-189.
Khairil. 2009. Lignoselulosa. Diakses dari
http://khairilfuture.multiply.com/journal/item/
Diunduh pada 6 Mei 2012.
Modi, HA. 2010. Regulation of Exoglucanase and
Endoglucanase Biosynthesis in Streptomyces
Species. Diakses dari : http://journal-advancesd
e v e l o p m e n t a l - r e s e a r c h . c o m / w p -
content/uploads/2010/06/Regulation-of-
E x o g l u c a n a s e - a n d - E n d o g l u c a n a s e -
Biosynthesis-in-Streptomyces-Species-Modi-
HA.pdf. Diunduh pada 6 Mei 2012.
Sambrook, J., Fritsch, E.F. and Maniatis, T. 1989.
Molecular Cloning I: A Laboratory Manual.
Cold Spring Harbor, NY: Cold Spring Harbor
Laboratory.
Soetrisno. 2008. Sumber bahan bakar hayati
langsung dari selulosa. Diakses dari :
http://www.chem-is-try.org/artikel_kimia/
kimia_material/sumber-bahan-bakar-hayatilangsung-
dari-selulosa/. Diakses pada : 6 Mei
Pecinta lingkungan hidup siklus ITS. 2010.
Mengembangkan bioetanol dari selulosa.
Diakses dari : http://siklus.lmb.its.ac.id/? p=71.
Diakses pada : 6 Mei 2012.
Daniel R. Zeigler, Zoltán Prágai, Sabrina Rodriguez,
Bastien Chevreux, Andrea Muffler, Thomas
Albert, Renyuan Bai, Markus Wyss and John B.
Perkins. 2008. The Origins of 168, W23, and
Other Bacillus subtilis Legacy Strains. Diakses
dari : http://jb.asm.org/content/190/21/6983.
abstract?ijkey=05bef 429089ec10c2d0a49
d1d7956b3c3f106bfc& keytype2=tf_ipsecsha.
Diakses pada : 10 Januari 2013.
DOI: https://doi.org/10.22146/jsv.8114
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