Deteksi Staphylococcus aureus dan Staphylococcus sp. secara Langsung dari Susu Segar Kambing Peranakan Etawa dengan Polymerase Chain Reaction (PCR)

Fatkhanuddin Aziz; Fajar Budi Lestari; Sarah Nuraida S; Endah Purwati; Siti Isrina Oktavia Salasia

doi:10.22146/jsv.60153

Deteksi Staphylococcus aureus dan Staphylococcus sp. secara Langsung dari Susu Segar Kambing Peranakan Etawa dengan Polymerase Chain Reaction (PCR)

https://doi.org/10.22146/jsv.60153

Fatkhanuddin Aziz^(1*), Fajar Budi Lestari⁽²⁾, Sarah Nuraida S⁽³⁾, Endah Purwati⁽⁴⁾, Siti Isrina Oktavia Salasia⁽⁵⁾

(1) Program Studi D3 Kesehatan Hewan, Departemen Teknologi Hayati dan Veteriner, Sekolah Vokasi, Universitas Gadjah Mada
(2) Program Studi D3 Kesehatan Hewan, Departemen Teknologi Hayati dan Veteriner, Sekolah Vokasi, Universitas Gadjah Mada
(3) Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(4) Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(5) Departemen Patologi Klinik, Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(*) Corresponding Author

Abstract

The genus of Staphylococcus is one of the bacterial pathogens that cause mastitis causing economic losses in Etawa crossbreed goat. Among Staphylococcus sp. which can grow well in raw milk, Staphylococcus aureus is known causing foodborn disease in human because of its ability to produce enterotoxins that are resistance to digestive enzymes or heating treatment. The purpose of this study was to detect Staphylococcus sp. and S. aureus directly from raw milk of Etawa crossbreed goat using PCR. The study was carried out by extracted DNA from fresh milk using spin column method and then amplified specific 23S rRNA for Staphylococcus sp. and S. aureus. PCR examination revealed that 37 (61%) and 1 (1,6%) raw milk samples were positive for Staphylococcus sp. and S. aureus respectively. The PCR Method is usefull to reduce defection time of Staphylococcus sp. contaminants.

Keywords

Goat; PCR; raw milk; Staphylococcus aureus; Staphylococus sp

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References

Achek, R., El-Adawy, H., Hotzel, H., Tomaso, H.,Ehricht, R., Hamdi, T. M.& Monecke, S.(2020). Diversity of staphylococci isolated from sheep mastitis in northern Algeria. Journal of Dairy Science. 103(1): 890-897.

Aziz, F. (2013). Determinasi Genetik Staphylococcus aureus Sapi Perah di Baturraden dan Pengembangan Deteksi Stafilokokal Mastitis Langsung dari Susu Segar dengan Polymerase Chain Reaction (PCR). Master Thesis. Program Studi 173 Bioteknologi, Sekolah Pascasarjana, UGM, Yogyakarta.

Aziz, F. Salasia, S.I.O., Artdita, C.A. dan Lestari, F.B. (2013). Pengembangan Deteksi Staphylococcus aureus Langsung dari Susu Segar dengan Polymerase Chain Reaction (PCR) dalam Prosiding Seminar Nasional Teknologi Terapan, 01 Oktober 2013,

Yogyakarta (ISBN 978-62-14066-2-5).

Boerema, J. A., Clemens, R. and Brightwell, G. (2006). Evaluation of molecular methods to determine enterotoxigenic status and

molecular genotype of bovine, ovine, human and food isolates of Staphylococcus aureus. International Journal of Microbiology. 107

(2): 192-201.

Budisatria, I. G. S., Maharani, D., & Ibrahim, A. (2019). Kambing Peranakan Etawah:Kepala Hitam atau Cokelat. UGM PRESS.

Cremonesi, P., Luzzana, M., Brasca, M., Morandi, S., Lodi, R., Vimercati, C., & Castiglioni, B. (2005). Development of a multiplex PCR assay for the identification ofStaphylococcus

aureus enterotoxigenic strains isolated from milk and dairy products. Molecular and cellular probes, 19(5): 299-305.

Coimbra-e-Souza, V., Rossi, C. C., Jesus-de Freitas, L. J., Brito, M. A. V., Laport, M. S., & Giambiagi-deMarval, M. (2019). Diversity of species and transmission of antimicrobial resistance among Staphylococcus spp. isolated from goat milk. Journal of dairy science. 102(6): 5518-5524.

Contreras, A., Sierra, D., Sánchez, A., Corrales, J.C., Marco, J. C., Paape, M. J., & Gonzalo, C. (2007). Mastitis in small ruminants. Small Ruminant Research. 68(1): 145-153.

Dash, H. R., Shrivastava, P., & Das, S. (2020). Isolation of DNA by Using Column-Based Extraction System. In Principles and

Practices of DNA Analysis: A Laboratory Manual for Forensic DNA Typing (pp. 103-108). Humana, New York, NY.

Fagundes, H., Barchesi, L., Filho, A. N., Ferreira, L. M. and Olieveira, C. A. F. (2010). Occurrence of Staphylococcus aureus in Raw Milk Produced in Dairy Farms in São Paulo State, Brazil. Brazilian Journal of Microbiology. (41): 376-380.

Haenlein, G. F. (2002). Relationship of somatic cell counts in goat milk to mastitis and productivity. Small ruminant research. 45(2): 163-178.

Harvey, J. and Gilmour, A. (1988), Isolation and characterization of staphylococci from goats milk produced in Northern Ireland.

Letters in Applied Microbiology. 7: 79–82.

He, Y., Liu, H., Xian, M. and Li, Y. (2010). Detection and Identification of Staphylococcus aureus in Raw Milk By Hybridization to Oligonucleotide Microarray. African Journal of Biotechnology. 9(15): 2284-2289.

Hayati, L. N., Tyasningsih, W., Praja, R. N.,Chusniati, S., Yunita, M. N., & Wibawati, P. A. (2019). Isolasi dan Identifikasi

Staphylococcus aureus pada Susu Kambing Peranakan Etawah Penderita Mastitis Subklinis di Kelurahan Kalipuro, Banyuwangi. Jurnal Medik Veteriner. 2 (2):76-82.

Nielsen, C. (2009). Economic Impact Of Mastitis in Dairy Cow. Doctoral Thesis. Swedish University of Agricultural Sciences

Uppsala. Petersson-Wolfe, C. S., Mullarky, I. K. and Andjones, G.M. (2010). Staphylococcus aureus Mastitis: Cause, Detection and Control. Virginia Cooperative Extension. Publication 404-229. www.ext.vt.edu. Diakses: 08 Mei 2019.

Phuektes, P., Mansell, P. D, Browning, G. F. (2001). Multiplex Polymerase Chain Reaction Assay For Simultaneous Detection

of Staphylococcus aureus and Streptococcal Causes of Bovine Mastitis. J Dairy Sci. 84 (5):1140–1148.

Purnomo, A., Hartatik, Khusnan, Salasia, S. I.,O. Dan Soegiyono. (2006). Isolasi dan,Karakterisasi Staphylococcus aureus Asal

Susu Kambing Peranakan Ettawa. Media,Kedokteran Hewan. 22 (3):142 – 147Fatkhanuddin Aziz, et. al. 174

Riffon, R., Sayasith, K., Lhalil, H., Dubreuil, P., Drolet, M. and Lagace, J. (2001). Development of A Rapid and Sensitive Test

For Identification of Major Pathogens in Bovine Mastitis by PCR. J. Clin. Microbiol. 39 (7): 2584–2589.

Salasia, S. I. O., Khusnan, Z., Lammler, C.,Zschock, M. (2004). Comparative studies on pheno-and genotypic properties of

Staphylococcus aureus isolated from bovine subclinical mastitis in central Java in Indonesia and Hesse in Germany. J Vet Sci. 5 (2): 103–109.

Salasia, S. I. O., Tato, S., Sugiyono, N., Ariyanti, D. and Prabawati, F. (2011). A Genotypic characterization of Staphylococcus aureus isolated from bovines, humans and food in Indonesia. J. Vet. Sci. 12(4): 353-361.

Straub, J. A., Hertel, C. and Hammes, W. P. (1999). A 23S rRNA-Targeted Polymerase Chain Reaction-Based System for Detection of Staphylococcus aureus in Meat Starter Cultures and Dairy Products. J. Food Prot. 62 (10):1150–1156.

Tan, A. and Hogg, G. (2008). Staphylococcus aureus and Food Borne Disease. Microbiology Australia. 29 (23): 155-156.

Taponen, S., Salmikivi, L., Simojoki, H., Koskinen, M. T. and Pyörälä, S. (2009). Real-Time Polymerase Chain ReactionBased Identification of Bacteria in Milk Samples From Bovine Clinical Mastitis With No Growth in Conventional Culturing.

J. Dairy Sci. 92 (6):2610–2617.

Todar, K. (2008). Staphylococcus aureus and Staphylococcal Disease; Todar’s Online Textbook of Bacteriology. www.textbook of bacteriology.net. Diakses : 08 Januari 2020.

Windria, S., Widianingrum, D. C., & Salasia, S. I. O. (2016). Identification of Staphylococcus aureus and Coagulase Negative

Staphylococci Isolates from Mastitis Milk of Etawa Crossbred Goat. Research Journal of Microbiology, 11(1): 11

DOI: https://doi.org/10.22146/jsv.60153