Hematology Profile and Liver Histopathology in Escherichia coli Infected Layers Treated with Combination of Phyllanthus ( Phyllanthus niruri L. ) and Turmeric ( Curcuma domestica )

https://doi.org/10.22146/jsv.58423

Sri Hartati(1), Tri Untari(2), Bambang Sutrisno(3), Ida Fitriana(4*)

(1) Department of Veterinary Clinical Science Faculty of Veterinary Medicine Universitas Gadjah Mada
(2) Department of Microbiology Faculty of Veterinary Medicine Universitas Gadjah Mada
(3) Department of Pathology Faculty of Veterinary Medicine Universitas Gadjah Mada
(4) Department of Pharmacology Faculty of Veterinary Medicine Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Colibacillosis a disease that can cause considerable economic loss, remains an important health problem. Phyllanthus (Phyllanthus niruri L) and turmeric (Curcuma domestica) are herbs that can be used as immunomodulators. This study was aimed to determine the level of safety of the combination of phyllanthus and turmeric on hematology profile and liver histopathology of layers with colibacillosis. The layers were assigned to the following of 5 groups: a) colibacillosis group without treatment, b) colibacillosis group with 500 mg/kg BW of phyllanthus, c) colibacillosis group with 300 mg/kg BW of turmeric, d) colibacillosis group with phyllanthus and turmeric combination (1:1), e) colibacillosis group with combination of phyllanthus and turmeric (1:2) . After 21 days of treatment, blood and liver sample were collected. The hematological profile (hemoglobin, hematocrit, erythrocyte and leukocyte counts) and liver histology were examined. The result were analyzed statistically by one-way ANOVA. The group that received phyllanthus had higher levels of hemoglobine, haematocrit and erythrocytes than the control group. However, no significant differences were found for the overall groups. Treatment with the combination of turmeric and phyllanthus for 21 days did not cause changes in the hematological profiles or liver histology, and therefor this herbal combination can be used as an alternative therapy for colibacillosis in layers.

Keywords


Colibacillosis; Layer; Phyllanthus; Turmeric

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References

Abalaka, S.E., Sani, N.A., Idoko, I.S., Tenuche, O.Z., Ovelowo, F.O., Eieh, S.A. and Enem, S.I. (2017). Pathological changes associated with an outbreak of colibacillosis in commercial broiler flock. Sokoto Journal of Veterinary Sciences, 15 (3): 95-102 Bagalkotkar, G., Sagineedu, S.R., Saad, M.S. and Stanslas, J. (2006). Phytochemicals from Phyllanthus niruri Linn. and their pharmacological properties: a review. J. pharma. and pharmacol., 58(12): 1559-1570 Çıkrıkçı, S., Mozioğlu, E. and Yılmaz, H. (2008). Biological Activity of Curcuminoids Isolated from Curcuma longa. Rec. Nat. Prod., 2(1): 19-24 Hasan, B., Faruque, R., Drobni, M., Waldenstrom, J., Sadique, A., Ahmed, K.U. and Alam, M. (2011). High Prevalence of Antibiotic Resistance in Pathogenic Escherichia coli from Large- and Small-Scale Poultry Farms in Bangladesh. Avian Diseases, 55 (4): 689–692 Hartati, S., Untari, T., Sutrisno, B., and Fitriana, I. (2018). Effects of Phyllanthus niruri and Curcuma longa on Liver and Renal Function of Escherichia coli Infected Layers. Asian Journal of Poultry Science, 12: 1-6. Jain, N.C. (1993). Essential of Veterinary Hematology. Philadelphia (US): Lea and Febriger. Kabir, S.M.L. (2010). Avian Colibacillosis and Salmonellosis: A Closer Look at Epidemiology, Pathogenesis, Diagnosis, Control and Public Health Concerns. Int. J. Environ. Res. Public Health, 7: 89-114. DOI:10.3390/ijerph7010089 Kamruzzaman, H.M. and Hoq, M.O. (2016). A review on ethnomedicinal, phytochemical and pharmacological properties of Phyllantus niruri. Journal of Medical Plants Studies, 4 (6): 173-180 Li, C., Dong, Y., Hou, H., Li, Q., Zhang, R., Qin, R., Li, Z., Bao, Y. and Shi, W. (2013). Effects of traditional Chinese herbal medicines on blood cell count and immunity in chickens. African Journal of Pharmacy and Pharmacology. Vol. 7(29), pp. 2081-2086, 2013. DOI 10.5897/ AJPP2013.3460 ISSN 1996-0816 Matin, M.A., Islam, M.A. and Khatun, M.M. (2017). Prevalence of colibacillosis in chickens in greater Mymensingh district of Bangladesh. Veterinary World, 10(1): 29–33. DOI: 10.14202/vetworld.2017.29-33; PMCID: PMC5301176 Meyer, D.J. and Harvey, J.W. (2004). Veterinary Laboratory Medicine Interpretation & Diagnosis. Third edition. Saunders, USA. Nakamura, K., Maeda, M., Imada, Y., Imada, T. and Sato, K. (1985). Pathology of spontaneous colibacillosis in a broiler flock. Vet Pathol, 22(6): 592-597 Shen, L. And Ji, H.F. (2012). The pharmacology of curcumin: Is it the degradation products?. Trends in Molecular Medicine, 18(3): 138-144 Solà-Ginés, M., Cameron-Veas, K., Badiola, I., Dolz, R., Majó, N., Dahbi, G., Viso, S., Mora, A., Blanco, J., Piedra-Carrasco, N., González-López, J.J. and Migura-Garcia, L. (2015). Diversity of multi-drug resistant avian pathogenic Escherichia coli (APEC) causing outbreaks of colibacillosis in broilers during 2012 in Spain. PLoS One, 10(11):e0143191. DOI: 10.1371/journal.pone.0143191. Suwarti, S.A. (2015). Pengaruh Faktor Sosial Ekonomi Rumah Tangga Terhadap Konsumsi Produk Peternakan Di Kecamatan Kasihan Kabupaten Bantul. Tesis. Universitas Gadjah Mada: Yogyakarta, pp: 2. Available at: http://etd.ugm.ac.id/index.php?mod=download&sub=DownloadFile&act=view&typ=html&id=81258&ftyp=potongan&potongan=S2-2015-325993-chapter1.pdf (Accessed 15 January 2018) Tian, W.X., Sun, N., Ning, G.B., Zhang, D.J., Feng, J. And Li, F. (2013). Effects of Gallid Herpesvirus 2 Marek's Disease Challenge Virus and Attenuated Vaccine Virus CVI988/Rispens on Immune Adhesion of Erythrocytes of Chickens. International Journal of Poultry Science 12 (4): 217-223, 2013. ISSN 1682-8356



DOI: https://doi.org/10.22146/jsv.58423

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