Uji Ekstrak n-Hexana Rumput Kebar (Biophytum petersianum Klotzsch) pada Tikus Wistar Hiperkolesterolemia

https://doi.org/10.22146/jsv.48487

Priyo Sambodo(1*), Purwaningsih Purwaningsih(2), Alnita Baaka(3), Trini Susmiati(4), Claude Mona Airin(5)

(1) Program Studi Diploma 3 Kesehatan Hewan, Fakultas Peternakan, Universitas Papua
(2) Program Studi Diploma 3 Kesehatan Hewan, Fakultas Peternakan, Universitas Papua
(3) Program Studi Diploma 3 Kesehatan Hewan, Fakultas Peternakan, Universitas Papua
(4) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(5) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(*) Corresponding Author

Abstract


This study was aimed to determine the secure level of the n-hexane extract of kebar grass on hypercholesterolemia rats based the acute oral toxicity test. Based the literature, no report on the toxic dosage before, initial dose was started at 300 mg/kg body weight (BW) and to be increased 2000 to 5000 mg/kg BW. Toxicity data were set for 24 hours and continued until 14 days after treatment for several parameters, i.e : toxicity symptoms, death of animals, changes in body weight and the manifestation of toxicity effects. At the end of the test, the rats were sacrificed and organs were taken for abnormalities (macroscopy). The results of this study showed that the administration of the n-hexane extract of grass kebar at all doses did not show the toxicity symptoms, mortality and body weight change. The absolute and relative weights and gross pathology observation of the internal organ were normal. Conclusions: the n-hexane extract of grass kebar is safe and the LD was determined on category 5, 50 unclassified or minimum practically non toxic

Keywords


grass kebar; hypercholesterolemia, rats

Full Text:

PDF


References

Banerjee, A., Al Rashid, H., Rahman, A., Pal, T.K. (2014). In-vivo screening of ethanolic extract of Biophytum sensitivum DC leaves on peptic ulcer induced by aspirin in Wistar albino rats. International Journal of P h a r m a c e u t i c a l a n d Phytopharmacological Research. 3(6): 418-422.

Bogoriani, N.W. (2008). Isolasi dan Identifikasi Glikosida Steroid dari Daun Andong (Cordyline terminalis Kunth). Jurnal Kimia 2 (1).40-44.

BPOM. (2014). Pedoman Uji Toksisitas Nonklinis secara in vivo. Badan Pengawas Obat dan Makanan RI.

Chapagain, B.P., and Wiesman, Z. (2005). Larvicidal Activity of the Fruit Mesocarp Extract of Balanites aegyptiaca and its Saponin Fractions against Aedes aegypti.

B. Dengue. 29. Diantika, L. N dan Wiwiek L., (2016). Uji toksisitas akut ekstrak etanol benalu mangga (Dendrophthoe petandra) terhadap mencit swiss webster. IJPST. 3 (2): 53-65

Dybing, E., Doe, J., Groten, J., Kleiner, J., Brien, J.O., Renwick, A.G. (2002). Hazard characterization of chemicals in food and diet: dose response, mechanism and extrapolation issues. Food Chem Toxicol. 42:237–282.

Giknis M.L.A dan Clifford, C.B. (2008). Clinical laboratory parameters for Crl: WI (Han). Charles River Laboratories. Montreal. 8.

Halim, S.Z., Abdullah, N.R., Afzan, A., Abdul Rashid, B.A., Jantan, I. Dan Ismail, Z. (2011). Acute toxicity study of Carica papaya leaf extract in Sprague Dawley rats. Journal of Medicinal Plants Research. 5(20): 18671872.

Kakade, R.T., Sandu, N and Senthilkumar, K.L. (2015). Evaluation of hypolipidemic and anti-obesity activities of Biophytum sensitivum Linn extracts on high fat diet induced hyperlipidemic rats. IJPCBS, 5(1): 357-360.

Mamoat, L.I. (2001). Minyak sawit mempercepat regresi aterosklerosis aorta pada kelinci hiperkolesterolemia ringan tetapi tidak apada yang hiperkolesterolemia berat. Tesis. Program Pascasarjana IPB. OECD. (2008).

OECD 407. Guidelines for the Testing of Chemicals. Repeated Dose 28-Day Oral Toxicity Study in Rodents. Oraon A. and Sinha B.N. (2012). Sub chronic toxicity potential of the alcoholic extract of Biophytum reinwardtii whole plant. Journal of Natural Sciences Research. 2(6): 94-100.

Rajeh, M.A.B., Kwan, Y.P, Zakaria, Z., Latha, L.Y., Jothy, S.L.,Sasidharan, S. (2012). Acute toxicity impacts of Euphorbia hirta L extract on behavior, organs body weight index and histopathology of organs of the mice and Artemia salina. Pharmacognosy Res. 4(3): 170–177.

Renuka, C., Elavarasi, S., Saravanan, K and Revathi, G. (2015). Antihyperlipidemic activity of Biophytum sensitivum extracts in streptozotocin (STZ) induced diabetic albino rats, Int J Pharm Bio Sci. 6(4): 128135.

Sambodo, P., Tethool, A.N. dan Rumetor, S.D. (2015). Efek antikolesterol fraksi n-Heksana Rumput Kebar pada hewan model hiperlipidaemia. Jurnal Kedokteran Hewan. 9 (1): 59-60.

Sambodo, P., Nurhayati D., Purwaningsih, Airin, C.M. dan Susmiati, T. (2016). Parameter eritrosit pada uji toksisitas subkronis ekstrak nhexana rumput Kebar (Biophytum petersianum Klotzsch) endemik Papua. Proceedings: Peran Dokter Hewan dalam Peningkatan Kesehatan Hewan, Lingkungan dan Manusia. FKH UGM. 111-113.

Sambodo, P., Nurhayati, D., Purwaningsih, Trini, S. dan Claude, M.A., (2018). Subchronic toxicity test of kebar grass extract of nHexane (Biophytium Petersianum Klotzcch) on weight, ADG, and vital organ of Rat (Rattus norvegicus).

J. Foof Pharm. Sci. 6 :14-16 Vaghasiya, Y.K., Shukla, V.J., Chanda, S.V. (2010). Acute oral toxicity study of Pluchea arguta boiss extract in mice. J Pharmacol Toxicol. 6:113–123.

Yunita, E.A., Suprapti N.H. dan Hidayat J.W. (2009). Pengaruh ekstrak daun Teklan (Eupatorium riparium) terhadap mortalitas dan perkembangan larva Aedes aegypti. BIOMA. 11. 1.



DOI: https://doi.org/10.22146/jsv.48487

Article Metrics

Abstract views : 1870 | views : 3699

Refbacks

  • There are currently no refbacks.




Copyright (c) 2019 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by

    CrossrefROADCOREProduct DetailsDESKRIPSI GAMBAR


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

HP. 0895363078367

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

free
web stats View My Stats