Efektifitas perlakuan awal Tretinoin 0,1% Topikal pada Proses Penyembuhan di Luka Insisi pada Mencit (Mus musculus)

https://doi.org/10.22146/jsv.41152

Iswinarno Doso Saputro(1*), Hesty Aulia Palupi(2), Wiwik Misaco Yuniarti(3)

(1) Fakultas Kedokteran Universitas Airlangga, Surabaya
(2) Fakultas Kedokteran Universitas Airlangga, Surabaya
(3) Fakultas Kedokteran Hewan Universitas Airlangga, Surabaya
(*) Corresponding Author

Abstract


Tretinoin has an important role in wound healing process include improve fibroplasia and collagen synthesis, maintening humoral immunity, and neutralize steroid effect.Epithelization  and fibroblast proliferation were very important event in wound healing process. The aims of this study were studying epithelization and fibroblas proliferation on incision wound healing process using pre treatment of  Tretinoin 0,1%  topically on mice. This research used 24 male mice, 3 month of age, with 150 -180 gram of body weight. The mice were divided into 2 groups, control group (K) and treatment group (P). The control group was a group of mice with an incision wound, while the treatment group was a group of mice with an incision wound that received a pretreatment of Tretionin of 0.1% topically. Each group was divided into 2 sub groups K4, K7, P4, P7 based on time of samples collecting. K4 and P4 were examined at  day 4, while K7 and P7 were examined at day 7. Epithelialization was observed by measuring the length of the epithelial incision area, whereas fibroblast proliferation was performed by counting the number of fibroblasts in five field of view on histopathologic skin preparations processed by HE staining.

In this study,pretreatment of Tretinoin 0,1%  topically can increase epithelialization and number of fibroblast significantly (p<0,05).This increase may be due to 1% Tretinoin in the tissues to induce keratinocyte basal proliferation including its migration to the skin surface via retinoic acid receptors ( RARδ)

Pretreatment of Tretinoin 0,1% topically can increased epithelialization and number of fibroblast on incision wound healing process of albino rats


Keywords


Tretinoin 0,1%; Epithelialization; Fibroblast; Wound incision

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References

Baxter C. 1997. The normal healing process. In: New Directions in Wound Healing. Wound care manual. Princeton, NJ: E.R. Squlbb & Sons, Inc. p. 80

Boonyong, T., Mayuree, Mattana, K., and Juraiporn, S. 2012. Woud Healing Activities of Different Extracts of Centella asiatica in Incision and Burn Wound Models : an experimental animal study. BMC Central and Alternative Medicine,

Danon, D., Kovatch, M.A., and Roth, G.S. 1989. Promotion of wound repair in old mice by local injection of monocytes/macrophages. Proc. Natl. Acad. Sci. USA Med Sci. 86: 2018–2020.

Elias PM, Williams ML. 1985. Retinoid effects on epidermal differentiation. In Saurat JH, Editor, Retinoids: new trends in research and therapy. Basel, Switzerland. S Karger. P 138

Elson, M.L. 1990. Treatment of striae distensae with topical tretinoin. J Dermatol Surg Onco 16 :3-10.

Elson, M.L. 1998. The role of retinoids in wound healing. J Am Acad Dermatol. 39 : 79-81.

Gorti, G. K., Ronson, S., and Koch, J. 2002. Wound healing. Facial Plastic Surg. Clin N Am. 10:119 -127

Gurtney, G. 2007. Wound Healing: Normal and Abnormal. Grabb and Smith's Plastic Surgery, Sixth Edition, 15-22.

Hans, T. 2011. Regulation of keratin expression by retinoids. Landes Bioscience : 136-140.

Hevia, O., Nemeth, A.J., and Taylor, J.R. 1991. Tretinoin accelerates healing after trichloracetic acid chemical peel. Arch Dermatol. 127;678

Hung, V.C.,Lee J.Y.Y, Hebda, P.A. 1989 .Arc Dermatol:vol 125: 65-69

Kurahashi T, Fujii. 2015. Roles of antioxidative enzymes in wound healing. J Dev Biol, 3 2:57-70.

Mackay, D. and Miller, A. 2003. Nutritional Support for Wound Healing. Alternative Medicine Review, 8(10):359-360.

Muehlberger, T., Moresi, J.M., Schwarze, H., Hristopoulos, G., Laenger, F., and Wong, L. 2004. The effect of topical tretinoin on tissue strength and skin components in murine incisional wound model. J of the Am Acad of Dermatol., 52 (4): 583-588

Paquette D, Badiavas E, Falanga V. 2001. Short-contact topical tretinoin therapy to stimulate granulation tissue in chronic wounds. J of the Am Acad of Dermtol., 45(3): 382-386

Peterson, A.L. 2003. Contemporary Oral and Maxillofacial Surgery : Wound Repair. 4th Ed. Mosby-Missouri, p. 51, 53-54



DOI: https://doi.org/10.22146/jsv.41152

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