* Corresponding Author
Kerusakan Usus pada Mencit (Mus musculus) yang Diinokulasi Larva 3 (L3) Anisakis spp.
https://doi.org/10.22146/jsv.29292 Febrina Dian Permatasari(1*), Poedji Hastutiek(2), Lucia Tri Suwanti(3)
(1) Fakultas Kedokteran Hewan, Universitas Airlangga
(2) Fakultas Kedokteran Hewan, Universitas Airlangga
(3) Fakultas Kedokteran Hewan, Universitas Airlangga
(*) Corresponding Author
Abstract
This study aims to be show damage in the intestine of mice caused by inoculation of the larvae of Anisakis spp. alive and who has died because of a warming 750C for 7 minutes. Eighteen male mice (Mus musculus) were divided into three groups: control group, a group of mice were inoculated the larvae of Anisakis spp. alive and
groups of mice were inoculated the larvae of Anisakis spp. who have died. 48 hours post inoculation, the mice’s intestines necropsy performed later performed HE staining to identify and scoring intestinal histopathology. The results showed inoculation of the larvae of Anisakis spp. either alive or dead induce histological changes in the intestine in the form of infiltration of inflammatory cells, epithelial changes and structural changes in the intestinal mucosa
Keywords
Full Text:
PDFReferences
Asami K and Inoshita Y (1967) Experimental
anisakiasis in guinea pigs: Factors influencing
infection of larvae in the host. Japanese J of
Parasitol 16: 415–422.
Audicana, T.M and Kennedy, M.W. 2008. Anisakis
simplex: from obscure infectious worm
toinducer of immune hypersensitivity. Clin
Microbiol Rev. 21(2):360-379.
Buzzell, G.R and Sommerville, R.I (1985) The structure
of the oesophagus in the third-stage infective
larva of Anisakis sp. (Nematoda: Anisakidae).
Trans Am Microsc Soc. 104: 86-94.
Caballero, M.L and Moneo, I (2004)Several allergens
from Anisakis simplex are highly resistant to heat
and pepsin treatments. Parasitol Res. 15:1-7.
Caballero, M.L. Moneo, I. Gomez-Aguado, F.
Corcuera, M.T. Casado, I and Rodriguez- Perez,
R (2008) Isolation of Ani s 5, an excretorysecretory
and highly heat-resistant allergen
useful for the diagnosis of Anisakis larvae
sensitization. Parasitol Res. 103(5):1231-1233.
Daschner, A. Cuéllar, C and Rodero, M.,2012. The
Anisakis allergy debate: does an evolutionar62
Febriana Dian Permatasari et al.
yapproach help? Trends Parasitol.28:9–15.
http://dx.doi.org/10.1016/j.pt.2011.10.001.
Diakses pada tanggal 7 Agustus 2016.
Erben, U. Loddenkemper, C. Doerfel, K.
Spieckermann, S. Haller, D. Heimesaat, M.M.
Zeitz, M. Siegmund, B and Kühl, A.A (2014)
A guide to histomorphological evaluation of
intestinal inflammation in mouse models. Int J
Clin Exp Pathol. 7(8):4557-4576.
Fukuda T, Tongu Y and Ishii A (1990) Ultrastructural
changes in the oesophagus, intestine,
and excretory organ of larval Anisakis
(Ascaridoidea: Nematoda) after incubation in
arti®cial gastric juice. Jpn J Parasitol 39: 1-6.
Gomez-Aguado, F. Picazo, A. Caballero, M.L.
Moneo, I. Asturias, J.A and Corcuera, M.T
(2003) Ultrastructural localization of Ani s 1, a
major allergen from the fish parasite Anisakis
simplex. Parasitol Res. 89:379–380.
Hariyadi, A.S (2006) Pemetaan infestasi cacing parasitik
dan risiko zoonosis pada ikan laut di perairan
Indonesia Bagian Selatan. Bogor. IPB.
Iglesias, L. Valero, A. Banitez, R. and Adroher, F.J
(2001) In vitro cultivation of anisakissimplex:
pepsin increases survival and moulting from
fourth larval to adult stage. Parasitol. 123:285-
Lindqvist, A. Ikezawa, Z. Tanaka, A and Yman,
L (1993) Seafood specific IgE in atopic
dermatitis. Ann Allergy. 70:58.
DOI: https://doi.org/10.22146/jsv.29292
Article Metrics
Abstract views : 8685
| 
views : 3821
Refbacks
- There are currently no refbacks.
Copyright (c) 2017 Jurnal Sain Veteriner
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Sain Veteriner Indexed by
Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).
Fakultas Kedokteran Hewan, Universitas Gadjah Mada
Jl. Fauna No.2, Karangmalang, Yogyakarta
Phone: 0274-560862
Fax: 0274-560861
Email: jsv_fkh@ugm.ac.id
HP. 0895363078367
Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.