* Corresponding Author
Molecular Analysis of Cytochrome Oxidase I in Bemisia tabaci (Gennadius) Populations Collected from Four Districts in the Special Region of Yogyakarta
https://doi.org/10.22146/jpti.75881 Nandini Ayuningtias(1), Witjaksono Witjaksono(2), Arman Wijonarko(3), Alan Soffan(4*)
(1) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281 Indonesia
(2) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281 Indonesia
(3) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281 Indonesia
(4) Department of Plant Protection, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora No. 1, Bulaksumur, Sleman, Yogyakarta 55281 Indonesia
(*) Corresponding Author
Abstract
Bemisia tabaci is an agricultural pest that interferes plant growth, as well as being an insect vector of various types of viruses, one of which is the geminivirus group. B. tabaci is called Cryptic Species Complex due to their similar morphology but has different genetic profiles. Climate change and increased global trading of agricultural products could increase B. tabaci population and lead to the emergence of genetic disparity. This study aims to obtain the latest information on the population homogeneity of B. tabaci in four districts of the Yogyakarta and potential differences on their nucleotide arrangements. Molecular identification was performed using PCR and primers C1-J-2198/L2-N-3914 on B. tabaci. B. tabaci COI gene sequences were then compared using a phylogenetic analysis and similarities of nucleotide bases were determined. Results showed that the populations of B. tabaci in Yogyakarta were still homogeneous showed by biotypes similarity, namely the non-B biotype. B. tabaci from Yogyakarta have nucleotide base similarity of 100% with B. tabaci from Singapore (AY686095) and Thailand (AY686092) and 99.56% with species from Bangladesh (AJ748388). Results also showed no differences in the composition of both nucleotide bases and amino acids from the four districts of B. tabaci sampling location. The homogeneous population of B. tabaci and the high incidence of yellowing disease caused by Begomovirus in chili pepper plants in the Special Region of Yogyakarta prove that it is necessary to review the current methods of controlling B. tabaci pests.
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de Barro, P.J., Liu, S.S., Boykin, L.M., & Dinsdale, A.B. (2011). Bemisia tabaci: A Statement of Species Status. Annual Review of Entomology, 56, 1–19. https://doi.org/10.1146/annurev-ento-112408-085504
Dinsdale, A., Cook, L., Riginos, C., Buckley, Y.M., & de Barro, P. (2010). Refined Global Analysis of Bemisia tabaci (Hemiptera: Sternorrhyncha: Aley- rodoidea: Aleyrodidae) Mitochondrial Cytochrome Oxidase 1 to Identify Species Level Genetic Boundaries. Annals of the Entomological Society of America, 103(2), 196–208. https://doi.org/10.1603/AN09061
Firdaus, S., Vosman, B., Hidayati, N., Supena, E.D.J., Visser, R.G.F., & van Heusden, A.W. (2013). The Bemisia tabaci Species Complex: Additions from Different Parts of the World. Insect Science, 20(6), 723–733. https://doi.org/10.1111/1744-7917.12001
Folmer, O., Black, M., Hoeh, W., Lutz, R., & Vrijenhoek, R. (1994). DNA Primers for Amplification of Mitochondrial Cytochrome C Oxidase Subunit I from Diverse Metazoan Invertebrates. Molecular Marine Biology and Biotechnology, 3(5), 294–299. Retrieved from https://www.researchgate.net/publication/15316743
Frohlich, D.R., Torres-Jerez, I., Bedford, I.D., Markham, P.G., & Brown, J.K. (1999). A Phylogeographical Analysis of the Bemisia tabaci species Complex Based on Mitochondrial DNA Markers. Molecular Ecology, 8(10), 1683–1691. https://doi.org/10.1046/j.1365-294X.1999.00754.x
Ganefianti, D.W., Hidayat, S.H., & Syukur, M. (2017). Susceptible Phase of Chili Pepper due to Yellow Leaf Curl Begomovirus Infection. International Journal on Advanced Science, Engineering and Information Technology, 7(2), 594–601. https://doi.org/10.18517/ijaseit.7.2.1872
Hidayat, P., Aidawati, N., Hidayat, S.H., & Sartiami, D. (2008). Tanaman Indikator dan Teknik RAPD- PCR untuk Penentuan Biotipe Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae). Jurnal Hama dan Penyakit Tumbuhan Tropika, 8(1), 1–7. https://doi.org/10.23960/j.hptt.181-7
Kalshoven, L.G.E. (1981). The Pests of Crops in Indonesia (P.A. van der Laan, Ed.). Jakarta, Indonesia: Ichtiar Baru.
Kusumaningrum, F., Hartono, S., Sulandari, S., & Somowiyarjo, S. (2015). Infeksi Ganda Begomovirus dan Crinivirus pada Tanaman Tomat di Kabupaten Magelang, Jawa Tengah. Jurnal Perlindungan Tanaman Indonesia, 19(2), 60–64. Retrieved from https://jurnal.ugm.ac.id/jpti/article/view/17542
Lestari, S.M., Hidayat, P., Hidayat, S.H., Shim, J.K., & Lee, K.Y. (2021). Bemisia tabaci in Java, Indonesia: Genetic Diversity and the Relationship with Secondary Endosymbiotic Bacteria. Symbiosis, 83(3), 317–333. https://doi.org/10.1007/s13199-021-00752-w
Nei, M., & Kumar, S. (2000). Molecular Evolution and Phylogenetics. New York, United States: Oxford University Press.
Nurulita, S., Hidayat, S.H., Mutaqin, K.H., & Thomas, J.E. (2015). Molecular Characterization of Begomovirus Infecting Yard Long Bean (Vigna unguiculata subsp. sesquipedalis L.) in Java, Indonesia. Biotropia, 22(1), 53–60. https://doi.org/10.11598/btb.2015.22.1.401
Perring, T.M. (2001). The Bemisia tabaci species Complex. Crop Protection, 20(9), 725–737. https://doi.org/10.1016/S0261-2194(01)00109-0
Rahayuwati, S., Hidayat, S.H, & Hidayat, P. (2016). Identitas Genetik Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae) dari Daerah Endemik Penyakit Kuning Cabai di Indonesia Bagian Barat Berdasarkan Fragmen Mitokondria Sitokrom Oksidase I (mtCOI). Jurnal Entomologi Indonesia, 13(3), 156–164. https://doi.org/10.5994/jei.13.3.156
Shadmany, M., Boykin, L.M., Muhamad, R., & Omar, D. (2019). Genetic Diversity of Bemisia tabaci (Hemiptera: Aleyrodidae) Species Complex Across Malaysia. Journal of Economic Entomology, 112(1), 75–84. https://doi.org/10.1093/jee/toy273
Simón, B., Cenis, J.L., Demichelis, S., Rapisarda, C., Caciagli, P., & Bosco, D. (2003). Survey of Bemisia tabaci (Hemiptera: Aleyrodidae) Biotypes in Italy with the Description of a New Biotype (T) from Euphorbia characias. Bulletin of Entomological Research, 93(3), 259–264. https://doi.org/10.1079/ber2003233
Simon, C., Frati, F., Beckenbach, A., Crespi, B., Liu, H., & Flook, P. (1994). Evolution, Weighting, and Phylogenetic Utility of Mitochondrial Gene Sequences and a Compilation of Conserved Polymerase Chain Reaction Primers. Annals of the Entomological Society of America, 87(6), 651–701. https://doi.org/10.1093/aesa/87.6.651
Srinivasan, R., Hsu, Y., Kadirvel, P., & Lin, M. (2013). Analysis of Bemisia tabaci (Hemiptera : Aleyrodidae) Species Complex in Java, Indonesia Based on Mitochondrial Cytochrome Oxidase I Sequences. The Philippine Agricultural Scientist, 96(3), 290–295.
Sulandari, S. (2006). Penyakit Daun Keriting Kuning Cabai di Indonesia. Jurnal Perlindungan Tanaman Indonesia, 12(1), 1–12. Retrieved from https:// jurnal.ugm.ac.id/jpti/article/view/11941
Tamura, K., Stecher, G., Peterson, D., Filipski, A., & Kumar, S. (2013). MEGA6: Molecular Evolutionary Genetics Analysis Version 6.0. Molecular Biology and Evolution, 30(12), 2725–2729. https://doi.org/10.1093/molbev/mst197
Tay, W. T., Evans, G. A., Boykin, L. M., & de Barro, P. J. (2012). Will the Real Bemisia tabaci Please Stand Up? PLoS ONE, 7(11), 7–11. https://doi.org/10.1371/journal.pone.0050550
DOI: https://doi.org/10.22146/jpti.75881
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