Aktivitas Antioksidan Ekstrak Metanol Kayu Mangifera indica L., Mangifera foetida Lour, dan Mangifera odorata Griff.

https://doi.org/10.22146/jik.3306

Ganis Lukmandaru(1*), Kristian Vembrianto(2), Anisa Alfiana Gazidy(3)

(1) Jurusan Teknologi Hasil Hutan, Fakultas Kehutanan, Universitas Gadjah Mada, Yogyakarta
(2) Alumni Fakultas Kehutanan, Universitas Gadjah Mada, Yogyakarta
(3) Alumni Fakultas Kehutanan, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author

Abstract


Ekstrak dari kayu dan kulit batang tiga spesies mangifera, yaitu mangga (Mangifera indica L.), pakel (Mangifera foetida Lour), dan kweni (Mangifera odorata Griff.) telah diuji aktivitas antioksidannya (AAO). Tiap bagian batang tersebut diekstrak dengan metanol (MeOH) dan ekstrak kasarnya kemudian difraksinasi secara bertingkat dengan pelarut n-heksana, etil asetat (EtOAc) dan n-butanol untuk memperoleh 4 fraksi berbeda. Sifat anti oksidan dari ekstrak MeOH dan hasil fraksinasinya ditentukan melalui uji 1,1-diphenyl-2-picryl-hydrazyl (DPPH). Penelitian ini menunjukkan bahwa ekstrak MEOH dari gubal pakel serta kulit kweni secara nyata memberikan AAO tertinggi jika dibandingkan dengan antioksidan standar (asam galat dan katekin) dengan nilai IC50 sekitar 3-10 ppm. Dari beberapa faksi yang dipisahkan pada ekstrak MeOH di bagian-bagian tersebut, fraksi terlarut EtOAc secara nyata menunjukkan AAO tertinggi. Uji identifikasi metabolit sekunder dengan reaksi kimia secara kualitatif mengindikasikan bahwa alkaloid dan tanin berperan pada hasil pengujian AAO. Kadar fenolat total (KFT) dari tiap ekstrak juga ditentukan berdasarkan metoda Folin-Ciocalteu. Baik pada bagian ekstrak kasar maupun hasil fraksinasi, hanya korelasi yang lemah didapatkan apabila antara nilai AAO dan KFT dihubungkan.

Kata kunci : Mangifera, ekstraktif, antioksidan, uji DPPH, kadar fenolat

 

Antioxidant Activity of Methanol Extracts from Mangifera indica L., Mangifera foetida Lour, and Mangifera odorata Griff.Woods

Abstract

Wood and bark extracts of three mangifera species, mangga (Mangifera indica L.), pakel (Mangifera foetida Lour), and kweni (Mangifera odorata Griff.) were examined for its antioxidant activity (AOA). Each stem part was extracted with methanol (MeOH) and the crude extract was then sequentially partitioned with n-hexane, ethyl acetate (EtOAc) and n-butanol to obtain four different fractions. The antioxidant properties of the MeOH extracts and their fractions were determined by 1,1-diphenyl-2-picrylhydrazyl (DPPH) assay. This study demonstrated that the MEOH extracts from the sapwood of pakel as well as the bark of kweni significantly exhibit the strongest AOA when compared to standard antioxidants (gallic acid and catechin) at approximately 3-10 ppm in IC50 values. Among the fractions separated from the methanol extracts of those parts, EtOAc soluble fractions significantly exhibited the highest AOA properties. Qualitative phytochemical tests of EtOAc soluble fractions indicated that alkaloid and tannin could contribute to the AOA results. Total phenolic contents (TPC) of each extract were also evaluated by Folin-Ciocalteu method. Both in the crude extracts and fractions stage, only weak correlation is found between the AOA and TPC levels.


Keywords


Mangifera; wood extractives; antioxidant; DPPH assay; phenolic content

Full Text:

PDF


References

  1. Andrianto D, Bintang M, Kustaman E, Katayama T, & Suzuki T. Effect of Eurycoma longifolia Jack on growth and testoterone level in male chicken. Proceedings of 1st Indonesia Wood Research Society. 2-3 November 2009, Bogor. Hlm. 230-235.
  2. Anjaneyulu¬ V, Satyanarayana P, Viswanadham KN, Jyothi VG, Rao K, & Radhika P. 1999. Triterpenoids from Mangifera indica. Phytochemistry 49:1229-1236.
  3. Diouf PN, Stevanovic T, & Cloutier A. 2009. Antioxidant properties and polyphenol contents of trembling aspen bark extracts. Wood Sci Technol 43: 457–470.
  4. Gao H, Shupe TF, Hse CY, & Eberhardt TL. 2006. Antioxidant activity of extracts from the bark of Chamaecyparis lawsoniana (A. Murray) Parl. Holzforschung 60: 459–462.
  5. Gulcin I, Elias R, Gepdiremen A, Taoubi K, & Koksal E. 2009. Antioxidant secoiridoids from fringe tree (Chionanthus virginicus L.). Wood Sci Technol 43:195–212.
  6. Kawamura F, Fatimah S, Ramle M, Sulaiman O, Hashim R, & Ohara S. 2011. Antioxidant and antifungal activities of extracts from 15 selected hardwood species of Malaysian timber. Eur. J. Wood Prod 69: 207–212.
  7. Lee JY, Yoon JW, Kim CT, & Lim ST. 2004. Antioxidant activity of phenylpropanoid esters isolated and identified from Platycodon grandiflorum. Phytochemistry 65 : 3033–3039.
  8. Núñez Sellés AJ, Vélez Castro HT, Agüero-Agüero J, González-González J, Naddeo F, De Simone F, & Rastrelli L. 2002. Isolation and quantitative analysis of phenolic antioxidants, free Sugars, and polyols from Mango (Mangifera indica L.) stem bark aqueous decoction used in Cuba as a nutritional supplement. J. Agric. Food Chem 50 : 762–766.
  9. Obst JR. 1998. Special (secondary) metabolites from wood. Dalam: Bruce A, Palfreyman JW (eds) Forest Products Biotechnology. Taylor & Francis. London. Hlm. 151–165.
  10. Pietarinen SP, Willför SM, Ahotupa MO, Hemming JE, & Holmbom BR. 2006. Knotwood and bark extracts. strong antioxidants from waste materials. J Wood Sci 52:436–444.
  11. Siramon P & Ohtani Y. 2007. Antioxidative and antiradical activities of Eucalyptus camaldulensis leaf oils from Thailand. J Wood Sci 53: 498–504.
  12. Susanti CME, Rahayu Y, Parubak AS & Lepong RL. 2008. Kandungan saponin pada tumbuhan Rhizophora mucronattta Lamk dan potensinya sebagai bahan pengawet alami kayu. Prosidings Seminar Nasional MAPEKI XI. Fakultas Pertanian Universitas Palangkaraya. Hlm. 328-339.
  13. Wang SY, Wu JH, Cheng SS, Lo CP, Chang HN, Shue LF, & Chang ST. 2004. Antioxidant activity of extracts from Calocedrus formosana leaf, bark, and heartwood. J Wood Sci 50: 422–426.
  14. Wang SY, Wu JH, Shyur LF, Kuo YH, & Chang ST. 2002. Antioxidant activity of abietane-type diterpenes from heartwood of Taiwania cryptomerioides Hayata. Holzforschung 56: 487–492.
  15. Zulaica-Villagomez H, Peterson DM, Herrin L, & Young R.A. 2005. Antioxidant activity of different components of pine species. Holzforschung 59: 156–162.



DOI: https://doi.org/10.22146/jik.3306

Article Metrics

Abstract views : 7209 | views : 13723

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2012 Jurnal Ilmu Kehutanan

License URL: https://creativecommons.org/licenses/by-nc-sa/4.0/

© Editorial Board Jurnal Ilmu Kehutanan
Faculty of Forestry, Universitas Gadjah Mada
Building D 2nd floor
Jl. Agro No 1, Bulaksumur, Sleman 55281
Phone. +62-274-512102, +62-274-550541, +62-274-6491420
Fax. +62-274-550541 E-mail : jik@ugm.ac.id
former website : jurnal.ugm.ac.id/jikfkt/
new website : jurnal.ugm.ac.id/v3/jik/

 

Indexed by:

 

Jurnal Ilmu Kehutanan is under the license of Creative Commons Attribution-ShareAlike 4.0 International