Pemberian kecambah kacang kedelai terhadap kadar malondialdehid (MDA) dan superoxide dismutase (SOD) tikus Sprague Dawley hiperkolesterolemia
Denny Indra Setiawan(1*), Kusmiyati Tjahyono(2), Diana Nur Afifah(3)
(1) Jurusan Gizi Politeknik Kesehatan Kementerian Kesehatan Gorontalo
(2) Program Studi Ilmu Biomedik, Fakultas Kedokteran Universitas Diponegoro
(3) Program Studi Ilmu Gizi, Fakultas Kedokteran Universitas Diponegoro
(*) Corresponding Author
Abstract
Background: Consumption of foods high in fat that happens in society can cause free radicals and trigger oxidative stress that results in the accumulation of fat cells in the adipose, cells damage and even cells death. An antioxidant activity that originates from food in the body, depends on the number of substances that can be absorbed and used to metabolism process. The selection of groceries that right can be alternatives management in hyperlipidemia. Soybean sprouts have properties that neutralize free radicals cause hyperlipidemia and cardiovascular diseases because it is an antioxidant compound.
Objective: The aim of this study was to determine the effect of soybean sprouts (Glycine Max) to levels of MDA and levels of SOD of male Sprague-Dawley rats.
Method: Research laboratory tests design post only controlled group design. The sample was 30 tailed rat Rattus norvegicus species Sprague Dawley. The rat 8-10 weeks and samples to be divided into 5 group. Group 1 was normal rat without treatment; groups II are hypercholesterolemic rat without treatment; groups III are hypercholesterolemic rat with treatment sprouts soybeans 0,53 g; group IV are hypercholesterolemic rat with treatment sprouts soybeans 1,06 g; the group V are hypercholesterolemic rat with treatment sprouts soybeans 2,12 g and all groups are treatment for 4 week.
Results: After four weeks of treatment, decline MDA levels in the hypercholesterolemic rat. Doses the provision of sprouts soybean 2,12 g/day is dosed most effective shown the significant differences (p<0.05) between the treatment group first to the treatment third group. Statistical analysis to SOD levels show differences meaningful in all the treatment group (p<0.05). Doses the provision of sprouts soybean 2,12 g/day proven effective against elevated levels of sod who demonstrated the significant differences (p=0,004) between the treatment group 1 (X1) to the treatment group 3 (X3).
Conclusion: Doses sprouts 2,12 g is dosed optimal in preventing elevated levels of MDA and help improve levels of SOD hypercholesterolemic Sprague-Dawley rats.
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- Badan Penelitian dan Pengembangan Kesehatan Kementerian Kesehatan Republik Indonesia. Riset Kesehatan Dasar (Riskesdas) tahun 2013. Jakarta: Kemenkes RI; 2013.
- Acha. National College Health Assessment: reference group executive summary spring 2013. [series online] 2013 [cited Juli 2015]. Available from: URL: http://www.acha-ncha.org/docs/ACHA-NCHA-II_ReferenceGroup_ExecutiveSummary_Spring2013.pdf.
- Wignjosoesastro C. Pengaruh bawang putih (Allium sativum) terhadap pencegahan hiperkolesterolemia pada tikus. Damianus J Med 2014;13(1):9-16.
- Z Chen, R Peto, R Collins, S MacMahon, J Lu, W Li, et al. Serum cholesterol concentration and coronary heart disease in population with low cholesterol concentrations. BMJ 1991;303(6797):276-82.
- Anggrahini S. Pengaruh lama pengecambahan terhadap kandungan α-tofoferol dan senyawa proksimat kecambah kacang hijau (Phaseolus radiatus L.). Agritech 2007;27(4):152-7.
- Yang RL, Shi YHS, Li W, Le G. Increasing oxidative stress with progressive hyperlipidemia in human: relation between malondialdehyde and atherogenic index. J Clin Biochem Nutr 2008;43(3):154-8.
- Etkin NL, Ross PJ. Food as medicine and medicine as food. An adaptive framework for the interpretation of plant utilization among the Hausa of Northern Nigeria. Soc Sci Med 1982;16(17):1559-73.
- Purwono, Hartono. Kacang hijau. Jakarta: Penyebar Swadaya; 2008.
- Morell SF. Dangers of dietary isoflavones at levels above those found in traditional diets. Food Chem Toxicol 2012;34:457-61.
- Purwandhono A. Pengaruh pemberian ekstrak tauge (Vigna radiata (L)) terhadap kadar kolesterol LDL dan perkembangan aterosklerosis pada tikus Wistar jantan hiperkolesterolemi. [series online] 2014 [cited Juli 2015]. Available from: URL: http://repository.unej.ac.id/handle/123456789/58898
- Yuan M, Jia X, Yang Y, Dinga C, Dub L, Chena Y, et al. Effect of light on structural properties and antioxidant activities of polysaccharides from soybean sprouts. Process Biochem 2015;50(7):1152-7.
- Wong ML, Tims RE, Goh EM. Colorimetric determination of total tocopherols in palm oil, olein and stearin. J Am Oil Chem Soc 1988;65;258.
- Daniels TF, Killinger KM, Michal JJ, Wright RW, Jiang Z. Lipoproteins, cholesterol homeostatis and cardiac health. Int J Biol Sci 2009;5(5):474-88.
- Lecumberri E, Goya L, Mateos R, Alía M, Ramos S, Bravo L, et al. A diet rich in dietary fiber from cocoa improves lipid profile and reduces malondialdehyde in hypercholesterolemic rats. Nutrition 2007;23(4):332-41.
- Yasmeen H, Hasnain S. In vitro antioxidant effect of Camellia sinensis on human cell cultures. Pak J Pharm Sci 2015;28(5):1573-81.
- Ibrahim KS, Saleh ZA, Farrag AR, Shaban EE. Protective effects of zinc and selenium against benzene toxicity in rats. Toxicol Ind Health 2011;26(6):537-45.
- McIntyre M, Bohr DF, Dominiczak AE. Endothelial function in hypertension: the role of superoxida anion. Hypertension 1999;34(4 Pt 1):539-45.
- Boaventura BC, Di Pietro PF, de Assis MA, Ambrosi C, Nesello LA, Fausto MA, et al. Antioxidant biomarkers and food intake in elderly women. J Nutr Health Aging 2012 Jan;16(1):21-5.
- Maryanto S, Fatimah S, Sugiri S, Marsono Y. The effect of red guava fruits supplementation on the SCFA and cholesterol production in caecum of hypercholesterolemic rats. Agritech 2013;33(3):334-9.
- Noeman SA, Hamooda HE, Baalash AA. Biochemical study of oxidative stress markers in the liver, kidney and heart of high fat diet induced obesity in rats. Diabetol Metab Syndr 2011;3(1):17.
DOI: https://doi.org/10.22146/ijcn.22815
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