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Perubahan parameter biologik jaringan kanker payudara mencit akibat pemberian isoflavon tempe
https://doi.org/10.22146/ijcn.18368 Siti Harnina Bintari(1*), Siti Fatimah Moeis(2), Sarjadi Sarjadi(3)
(1) Universitas Negeri Semarang
(2) Bagian Gizi Klinik, Program Pendidikan Dokter Spesialis, Fakultas Kedokteran Universitas Diponegoro
(3) Bagian Gizi Klinik, Program Pendidikan Dokter Spesialis, Fakultas Kedokteran Universitas Diponegoro
(*) Corresponding Author
Abstract
Background: Cancer is severe diseases caused by the changing of deoxyribonucleic acid (DNA) structure. Women who consume soy products have a lower risk for breast cancer, but it cannot be explained the kind of biomolecular aspects of soy product which gives this role.
Objective: The aim of this research was to analyze the biological parameter changing of AgNORs, p53, Cas-3 and Bcl-2 in mouse (Mus musculus) C3H strain with breast cancer, as the impact of injecting isoflavone of tempeh.
Method: The laboratory experimental research was done by posttest only randomized controlled group design. Subject of study were 18 mice (Mus musculus) C3H strain that were divided into 3 groups, each of which consists of 6 mice. The first group, the control (K), was not injected by isoflavone, while the second group (P1) and the third group (P2) were injected by 1.000 mg and 10.000 mg of tempeh isoflavone/kg diet/day, respectively. All groups were given the transplanted breast cancer cells. AgNORs parameters were selected to determine the level of proliferation and p53 parameters, Cas-3 and Bcl-2 to determine the appearance of apoptosis. Data were analyzed by using Manova and discriminant test for each parameter of cell proliferation.
Results: Concentration of isoflavones supplementation with 1,000 and 10,000 mg / kgdiet / day gave effect to the reduction of AgNORs blots, expression of p53 and Cas-3 and increased expression of Bcl-2 in group P1 and P2 compared with control group, as indicated by high statistical values (p=0.000) between both groups or within the group. Results of discriminant test showed that Bcl-2 and AgNORs had dominant role in increased apoptosis and inhibition of cancer cell proliferation in experimental animal.
Conclusion: Tempeh isoflavones can be used as a biological parameter change agent that marks the proliferation and apoptosis of mice (Mus musculus) C3H strain towards the normal cell cycle progression.
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Marchbanks PA, McDonald JA, Wilson HG, Folger SG, Mandel MG, Daling JR, Bernstein L, Malone KE, Ursin G, Strom BL, Norman SA, Wingo PA, Burkman RT, Berlin JA, Simon MS, Spirtas R, Weiss LK. Oral contraceptives and the risk of breast cancer. N Engl J Med 2002;346(26):2025-32.
McPherson K, Steel CM, Dixon JM. ABC of breast diseases. Breast cancer–epidemiology, risk faktor, and genetics. BMJ 2000;321(7261):624-8.
Birt DF, Shull JD, Yaktine AL. Chemoprevention of cancer. Within modern nutrition in health and disease. Ninth ed. New York: Lippincott Williams & Wilkins; 2005.
Bruneton J. Essential Oils. Pharmacology: phytochemistry medicinal plants. 2nd ed. Paris: Intercept Ltd; 1999.
Toews VD. All about soy isoflavones & women’s health. Garden City Park: Avery Publishing Group; 1999.
Onozawa M, Kawamori T, Baba M, Fukuda K, Toda T, Sato H, Ohtani M, Akaza H, Sugimora T, Wakabayashi K. Effect of a soybean isoflavone mixture on carcinogenesis in prostate and seminal vesicles of F344 rats. Jpn J Cancer Res 1999;90(4):393-8.
Folmer F. Apoptosis – a review. [series online] 2000 [cited 2003 April 3]. Available from: URL: http://www.geocities.com/Collegepark/Lab/1580/Apoptosis.html
Kaplan R, Fisher DE. Apoptosis and cancertherapy. [series online] 2000 [cited 2004 March 23]. Available from: URL: http//www.eurekah. com/ reports/cellcycle/ha
Prall OW, Rogan EM, Musgrove EA, Watts CK, Sutherland RL. c-Myc or cyclin D1 mimics estrogen effects on cyclin E-Cdk2 activation and cell cycle reentry. Mol Cell Biol 1998;18(8):4499-508.
Vile RG, McClure MO, Weber JN. Genes and cancer. Basic molecular and cell biology. 2nd ed. Tavistock Square, London: BMJ Publishing Group; 1993.
Musgrove EA, Swarbrick A, Lee SCL, Cornish AL, Sutherland RL. Mechanisms of cyclin-dependent kinase inactivation by progestins. Mol Cell Biol 1998;18(4):1812-825.
Neuman E, Ladha MH, Lin N, Upton TM, Miller SJ, DiRenzo J, Pestell RG, Hinds PW, Dowdy SF, Brown M, Ewen ME. Cyclin D1 stimulation of estrogen receptor transcriptional activity independent of cdk4. Mol Cell Biol 1997;17(9):5338-47.
Hillman GG, Wang Y, Kucuk O, Che M, Doerge DR, Yudelev M, Joiner MC, Marples B, Forman JD, Sarkar FH. Genistein potentiates inhibitors of tumor growth by radiation in a prostate cancer orthotopic model. Mol Cancer Ther 2004;3(10):1271-9.
Worsley SD, Jennings BA, Khalil KH, Mole M, Girling AC. Cyclin D1 amplification and expression in human breast carcinoma: correlation with histological prognostic markers and oestrogen receptor expression. Clin Mol Pathol 1996;49(1):M46-M50.
Surh Y-J. Cancer chemoprevention by dietary phytochemicals; a mechanistic viewpoint. Cancer J 1998;11(1):6-10.
Clark DP, Russel LD. Moleculer biology. USA: Cache River Press; 1997.
Surh Y-J, Na H-K. Cyclooxygenase-2 as a putative target for cancer chemoprevention some anti inflammatory phytochemicals. Food Chem Toxicol 2002;40:146-51.
Bintari SH. Efek isoflavon tempe pada proliferasi kanker mama mencit (Mus musculus) galur C3H (studi pendahuluan). National Seminar on Medical Biotechnology and its Related Fields: Link Beetween Pharmacetical and Research Universities: Myth or Reality by Medical Biotechnology Laboratory Medical Faculty Diponegoro University in Collaboration with Indonesia; 2007 Jan 27; Semarang.
Astawan dan Maskar. Nilai gizi kedelai serta manfaat kesehatan konsumsi kedelai dan produk olahannya. Makalah Pelatihan dan Pengembangan Kuliner Berbasis Tempe; 2011 Okt 9; Pekalongan.
Surh Y-J. Anti-tumor promoting potential of selected spice ingredients with antioxidative and anti-inflammatory activities: a short review. Food Chem Toxicol 2002;40(8):1091-7.
Griffiths AJF, Miller JH, Suzuki DT, Lewontin RC, Gelbart WM. An introduction to genetic analysis. Sixth ed. New York: W.H Freeman and Company; 1996.
Berdanier CD. Advanced nutrition micronutrients. New York: CRC Press; 1998.
Toyomura K, Kono S. Soybean, soy foods, isoflavones and risk of colorectal cancer: a review of experimental and epidemiological data. Asian Pac J Cancer Prev 2002;3(2):125-132.
Barnes S. Role of phytochemicals in prevention and treatment of prostat cancer. Epidemiol Rev 2001;23(1):102-5.
Jha HC, Kiriakidis S, Hoppe M, Egge H. Antioxidative constituents of tempe. Reinventing the hidden miracle of tempe. Proceedings International Tempe Symposium; 1997 July 13 -15; Bali, Indonesia. Jakarta, Indonesia: Indonesian Tempe Foundation; 1997.
Surh Y-J. Molecular mechanisms of chemopreventive effects of selected dietary and medicinal phenolic substances. Mutat Res 1999;428(1-2):305-27.
Milner JA, McDonald SS, Anderson DE, Greenwald F. Moleculer targets for nutrients involved with cancer prevention. Nutr Cancer 2001;41(1-2):1-16.
Djuanda DG. Penelitian penularan virus tumor mamma (Mtv) pada mencit C3H. Bulletin Penelitian Kesehatan 1974;2(1):26-32.
DOI: https://doi.org/10.22146/ijcn.18368
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