Antioxidant and antibacterial activity of Acorus calamus. L leaf and rhizome extracts

https://doi.org/10.22146/ijcn.17937

Kho See Li(1*), Chan Sook Wah(2)

(1) School of Bioscience, Taylor’s University, Selangor
(2) School of Bioscience, Taylor’s University, Selangor
(*) Corresponding Author

Abstract


Background: Acorus calamus (sweet flag) is a well-known traditional herb used in India. There were only limited studies done on both hydrophilic and hydrophobic extracts of A. calamus and the biological activities of phenolic and flavonoid compounds as well as alpha-asarone in the sweet flag.

Objective: The present study was designed to explore the antioxidant and antibacterial activities of both hydrophilic and hydrophobic of A. calamus leaf and rhizome extracts. Lastly, the correlation between the biological activities and total phenolic content (TPC), total flavonoid content (TFC) as well as total alpha-asarone content (TAC) of three different extracts (water, hexane and methanol extracts) was revealed.

Method: TAC was determined through using HPLC method, while TPC and TFC were determined through spectrophotometric method. DPPH-free radical scavenging assay (DPPH-FRS) and ferric reducing power assay (FRAP) were used to analyze antioxidant activity, whereby disc diffusion method was used to analyze the antibacterial activity.

Results: Methanol extracts had significantly (p<0.05) highest levels of antioxidant activities, antibacterial activity TPC (4.466%1; 3.603%2) and TFC (12.819%1; 7.411%2) content. The significant (p<0.05) highest TAC was observed in hexane extracts (0.762%1; 2.975%2). TPC (p<0.05; r=0.855) and TFC (p<0.05; r=0.855) correlated to FRAP, while DPPH-FRS solely correlated to TFC (p<0.05; r=0.873).

Conclusion: Both of the hydrophilic and hydrophobic extracts of A. calamus could be potent antioxidant agents for nutraceutical application and antibacterial agent for food application. Lastly, flavonoids and phenols extracted from sweet flag could be a good potent source of antioxidants for nutraceuticals or food additives as those compounds are correlated to the antioxidant activity.

1 Leaf extract; 2Rhizome extract


Keywords


A. calamus; alpha-asarone; antibacterial; antioxidant; flavonoids; nutraceuticals; phenols; sweet flag

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References

  1. Gupta AD, Bansal BP, Babu, B, Maithil, N. Antioxidant and antimicrobial potential of nutmeg (Myristica fragrans Houtt). Journal of Genetic Engineering and Biotechnology 2011;11(1):25-31.
  2. Shukla R, Singh P, Prakash B, Dubey NK. Efficacy of Acorus calamus L.essential oil as a safe plant based antioxidant, aflatoxin B1 suppressor and broad spectrum antimicrobial against food-infesting fungi. International Journal of Food Science and Technology 2013;48:128-35.
  3. Nasri H, Baradaran A, Shirzd H, Rafieian-Kopaei M. New concepts in nutraceuticals as alternative for pharmaceuticals. US National Library of Medicine National Institutes of Health 2014;5(12):1487-99.
  4. Institute for Public Health (IPH). National health health and morbidity survey 2014: Malaysian Adult Nutrition Survey Vol II: survey finding. Malaysia: Ministry of Health Malaysia; 2014.
  5. Lobo V, Patil A, Phatak A, Chandra N. Free radicals, antioxidants and functional foods: impact on human health. US National Library of Medicine National Institutes of Health 2010;4(8):118-26.
  6. Rajput SB, Karuppayil SM, Tonge MB. An overview on traditional uses and pharmacological profile of. (sweet flag) and other Acorus species’ phytomedicine. International Journal of Phytotherapy and Phytophamacology 2014;21(3):268-76.
  7. Tiwari N, Chaudhary A, Mishra U. Enthnopharmacological aspect of Acorus calamus: a review. Pharmacologyonline 2010;2:435-45.
  8. Mahmoudi S, Khali M, Benkhaled A, Benamirouche K, Baiti I. Phenolic and flavonoid contents, antioxidant and antimicrobial activities of leaf extracts from ten Algerian ficus carica L. varieties. Asia Pacific Journal of Tropical Biomedicine 2016;6(3):239-45.
  9. Manach C, Scalbert A, Morand C, Remesy C, Jimenez, L. Polyphenols: food sources and bioavailability. Am J Clin Nutr 2004:79(5):727-47.
  10. Gropper SS, Smith JL, Groff JL. Advanced nutrition and human metabolism: phytochemicals and herbal supplements in health and disease. Canada: Cengage Learning; 2009.
  11. Nandakumar S, Menon S, Shailajan S. A rapid HPLC-ESI-MS/MS method for determination of β-asarone, a potential antiepileptic agent in plasma after oral administration of Acorus calamus extract to rats. US National Library of Medicine 2013;3(3):18-26.
  12. Shailajan S, Menon S, Swar G, Singh D, Nair S. Estimation of quantitation of β-asarone from Acorus calamus rhizome and its formulations using validated RP-HPLC method. Pharmaceuticals Method 2015;6(2):94-4.
  13. Liu X, Zhao M, Wang J, Yang B, Jiang Y. Antioxidant activity of methanolic extract of emblica fruit (Phyllantbus emblica L.) from six regions in China. J Food Compt Anal 2008;21(3):219-28.
  14. Jia Z, Tang M, Wu J. The determination of flavonoid contents in Mulberry and their scavenging effects on superoxide radicals. Food Chem 1999;64(4):555-99.
  15. Devi SA, Ganjewala D. Antioxidant activities of methanolic extracts of sweet flag (Acorus calamus) leaves and rhizomes. Journal of Herbs, Spices & Medicinal Plants 2011;17(1):1-11.
  16. Oyaizu M. Studies on products of browning reactions: antioxidant activities of products of browning reaction prepared from glucosamine. J Nutrit 1986;44:307-15.
  17. Devi SA, Ganjewala, D. Antimicrobial activity of Acorus calamus (L).rhizome and leaf extract. Acta Biological Szegediensis 2009;53(1):45-9.
  18. Balakumbahan R, Rajamani K, Kumanan, K. Acorus calamus: an overview. Journal of Medicinal Plants Research 2010;4(25):2740-5.
  19. Raja AE, Vijayalakshmi M, Devalarao G. Acorus calamus Linn.: chemistry and biologiy. Research Journal of Pharmacy and Technology 2009;2(2):256-61.
  20. Ophardt CE. Polarity of organic compounds, Virtual Chemvbook. [series online] 2013 [cited 2016 September 16]. Available from: URL: http://chemistry.elmhurst.edu/vchembook/213organicfcgp.htm
  21. Sigma-Aldrich. Alpha-asarone. Figure 1: Alpha-asarone structure [series online] 2016 [cited 2016 September 16]. Available from: URL: http://www.sigmaaldrich.com/catalog/product/aldrich/231282?lang=en&region=MY
  22. Devaki M, Nirupama R, Nirupama M, Yajurvedi HN. A protective effect of rhizome extracts of the herb, vacha (Acorus calamus) against oxidative damage: an in vivo and in vitro study. Food Science and Human Wellness 2016;5:76-84.
  23. Soderberg T. Solubility, melting points and boiling points. LibreTexts. [series online] 2016 [cited 2016 October 6]. Available from: URL: https://chem.libretexts.or
  24. George AV. Alcohols, phenols and ethers. [serial online] 2006 [cited 2014 November 13]. Available from: URL: http://sydney.edu.au/science/chemistry/~george/alcohols.htm
  25. Khoddami A, Wilkes MA, Roberts TH. Techniques for analysis of plant phenolic compounds. Molecules 2013;18(2):2328-75.
  26. Kedare SB, Singh RP. Genesis and development of DPPH method of antioxidant assay. Journal of Food Science and Technology 2011;48(4):412-22.
  27. Amic D, Davidovi-Amic D, Besl D, Trinajstic N. Structure-radical scavenging relationships of flavonoids. Croat Chem Acta 2002;76(1):55-61.
  28. Devi SA, Mali AL, Rahee MA, Belinda EDS. Antioxidant Properties of Alpha Asarone. Asian Journal of Biochemistry 2014;9:107-13.
  29. Gabriel AA, Joe A, Vinson, JA, Donnelly PE. Folin-ciocalteau reagent for polyphenolic assay. Int J Food Sci Nutr Diet 2014;3(8):147-56.
  30. Koolen HHF, da Silva FMA, Gozzo FC, de Souza AQL, de Souza, ADL. ‘Antioxidant, antimicrobial activities and characterization of phenolic compounds from buriti (Mauritia flexuosa L. f.) by UPLC–ESI-MS/MS’. Food Research International 2013;51(2):467-73.
  31. Joshi N, Prakash O, Pant AK. Essential oil composition and in vitro antibacterial activity of rhizome essential oil and β-asarone from Acorus calamus L. collected from lower Himalayan Region of Utarakhand. Journal of Essential Oil Bearing Plants 2012;15(1):32-7.
  32. Phongpaichit S, Pujenjob N, Rukachaisiriku V, Ongsakul M. Antimicrobial activity of the crude methanol extract of Acorus calamus Linn. J Sci Technol 2005;27(2):517-23.
  33. Ravn H, Andary C, Kavacs G, Molgaard, P. Caffeic acid as in vitro inhibitors of plant pathogenic bacteria and fungi. Biochemistry System and Ecology 1989;17:174-84.
  34. Singh S, Srivastava R, Choudhary S. Antifungal and HPLC analysis of the crude extracts of Acorus calamus, Tinospora cordifolia and Celestrus paniculatus. Journal of Agricultural Technology 2010;6(1):149-58.
  35. Souza JE, Casanova LM, Costa SS. Bioavailability of phenolic compounds: a major challenge for drug development?’. Revista Fitos, Rio de Janeiro 2005;9(1):1-72.
  36. Hayek SA, Gyawali, R, Ibrahim SA. Antimicrobial natural products’. FORMATEX; 2013.



DOI: https://doi.org/10.22146/ijcn.17937

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