* Corresponding Author
The Effect of Gracilaria Liquid Organic Fertilizer Fermented with Different Starters on the Growth of Pakcoy
https://doi.org/10.22146/jfs.93277 Eko Nurcahya Dewi(1*), Aulia Rahmawati Tsaniya(2), Lukita Purnamayati(3)
(1) Department of Fish Product Technology, Faculty of Fisheries and Marine Sciences, Universitas Diponegoro, Semarang, Central Java
(2) Department of Fish Product Technology, Faculty of Fisheries and Marine Sciences, Universitas Diponegoro, Semarang, Central Java
(3) Department of Fish Product Technology, Faculty of Fisheries and Marine Sciences, Universitas Diponegoro, Semarang, Central Java
(*) Corresponding Author
Abstract
This research aimed to determine the effect of adding three different starters, such as Lactobacillus plantarum, Azospirillum sp., and Trichoderma sp., on gracilaria seaweed for bok choy growth. The starter optimized fermentation and improved fertilizer quality in growing bok choy. The seaweed was reduced in size, soaked in 1% phosphoric acid solution, then homogenized and heated with 3% molasses to provide microbial nutrition and 1% KOH to maximize mineral content. Fermentation was carried out for 14 days in the microbial exponential phase for decomposition. The results showed that the addition of L. plantarum and Trichoderma sp. to gracilaria seaweed liquid fertilizer produces the highest values of gibberellin (147 ppm), auxin (140 ppm), and cytokinin (96 ppm). Next, the fertilizer was applied to bok choy to determine the effect on its growth. The highest fresh yield of bok choy was 64.0±1.41 g, the highest chlorophyll A content was 6.70±0.01%, and chlorophyll B was 2.30±0.03%. Even though the control treatment had the highest C/N ratio compared to the other treatments, the highest yields of bok choy freshness, height, and leaf area were found when applying liquid fertilizer with L. plantarum and Trichoderma sp. starter. The study concluded that bok choy growth was significantly encouraged by fertilizer-seaweed with high levels of phytohormones compared to mineral content. Adding L. plantarum and Trichoderma sp. is a good starter for increasing the growth-promoting properties of liquid seaweed fertilizer.
Keywords
References
Abdulgawad, I.A.I. 2016. Fermentation of nile tilapia (Oreochromis niloticus) wastes using lactobacillus plantarum for the production of lactic acid and fertilizer. International Journal of Waste Resources. 6 (4): 1-7. https://doi.org/10.4172/2252-5211.1000253
Alamsjah, M.A. 2013. Gracilaria sp. waste, Lactobacillus sp. and Chlorella sp. integration on intensive aquaculture with aquaponic system. Journal of Natural Sciences Research. 3 (11): 66-78.
Ammar, E.E., A.A.A. Aioub, A.E. Elesawy, A.M. Karkour, M.S. Mouhamed, A.A. Amer & N.A. El-Shershaby. 2022. Algae as biofertilizers: Between current situation and future prospective. Saudi Journal of Biogical Sciences 29: 3082-3096.
AOAC. 2012. Official Methods of Analysis of AOAC International. 19ed.
Bhaskoro, P., Tjahjaningsih, W., & Mubarak, A. (2020). The effect of addition of fish bone meal on the concentration of nitrogen (N), phosphorus (P), and potassium (K) in seaweed liquid organic fertilizer of Gracilaria sp. IOP Conference Series: Earth and Environmental Science, 441, 1-6. https://doi.org/10.1088/1755-1315/441/1/012144
Dewi, E. N., Rianingsih, L., & Anggo, A. D. (2019). The addition of different starters on characteristics Sargassum sp. liquid fertilizer. IOP Conference Series: Earth and Environmental Science, 246, 1-7. https://doi.org/10.1088/1755-1315/246/1/012045
Dewi, E., Septiningrum, S., Rianingsih, L., & Riyadi, P. (2020). Optimization of carbon source and concentration for Lactobacillus acidophilus growth, phenolic production and antioxidant activity in fermented seaweed extract. International Journal of Emerging Technologies in Learning (iJET), 11(5), 495-500.
Dewi, T. K., Arum, E. S., Imamuddin, H., & Antonius, S. (2015). Characterization of plant growth promoting Rhizobacteria (PGPR) supporting organic biofertilizer. Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia, 1(2), 289-295.
Egamberdieva, D., Wirth, S. J., Alqarawi, A. A., Abd_Allah, E. F., & Hashem, A. (2017). Phytohormones and beneficial microbes: essential components for plants to balance stress and fitness. Frontiers in microbiology, 8, 1-14. https://doi.org/10.3389/fmicb.2017.02104
Galindo, F. S., Pagliari, P. H., da Silva, E. C., Silva, V. M., Fernandes, G. C., Rodrigues, W. L., Céu, E. G. O., de Lima, B. H., Jalal, A., & Muraoka, T. (2022). Co-Inoculation with Azospirillum brasilense and Bradyrhizobium sp. enhances nitrogen uptake and yield in field-grown cowpea and did not change n-fertilizer recovery. Plants, 11(14), 1847. https://doi.org/10.3390/plants11141847
Godlewska, K., Michalak, I., Tuhy, Ł., & Chojnacka, K. (2016). Plant growth biostimulants based on different methods of seaweed extraction with water. BioMed research international, 2016. https://doi.org/10.1155/2016/5973760
Harlis, H., Budiarti, R. S., Kapli, H., & Sanjaya, M. E. (2019). Produksi pupuk cair dari isolat bakteri limbah sayur pasar angso duo jambi dalam meningkatkan perekonomian dan kesehatan lingkungan masyarakat Jambi. Biospecies, 12(1), 40-48. https://doi.org/10.22437/biospecies.v12i1.6577
Kusumawati, R., Nurhayati, Pangestu, H. E., & Basmal, J. (2021). Effect of Trichoderma addition on Sargassum organic fertilizer. IOP Conf. Series: Earth and Environmental Science, 715, 1–5. https://doi.org/10.1088/1755-1315/715/1/012059
Lin, H.-T. V., Huang, M.-Y., Kao, T.-Y., Lu, W.-J., Lin, H.-J., & Pan, C.-L. (2020). Production of lactic acid from seaweed hydrolysates via lactic acid bacteria fermentation. Fermentation, 6(1), 1-11. https://doi.org/10.3390/fermentation6010037
Mangmang, J. S., Deaker, R., & Rogers, G. (2015). Azospirillum brasilense enhances recycling of fish effluent to support growth of tomato seedlings. Horticulturae, 1, 14–26. https://doi.org/10.3390/horticulturae1010014
Ministry of Agriculcure. (2019). Persyaratan Teknis Minimal Pupuk Organik, Pupuk Hayati, dan Pembenah Tanah. Minister of Agriculture of Republic Indonesia. Jakarta. (p. 18).
Mutryarny, E., & Lidar, S. (2018). Pakcoy plant response (Brassica rapa L) due to hormone growth regulators]. Jurnal Ilmiah Pertanian, 14(2), 29-34. https://doi.org/10.31849/jip.v14i2.258
Nasmia, Rosyida, E., Masyahoro, A., Putera, F., & Natsir, S. (2021). The utilization of seaweed-based liquid organic fertilizer to stimulate Gracilaria verrucosa growth and quality. International Journal of Environmental Science and Technology, 18(6), 1637-1644. https://doi.org/10.1007/s13762-020-02921-8
Nurhayati, Cahyaningtyas, W., Kusumawati, R., & Basmal, J. (2021). Effect of molasses on the chemical characteristics of seaweed-based organic fertiliser. IOP Conf. Series: Earth and Environmental Science, 733, 1–8. https://doi.org/10.1088/1755-1315/733/1/012110
Pangaribuan, D. H., Ginting, Y., Rugayah, R., Oktiya, R., & Zaheri, E. D. (2022). The effect of seaweed (Sargassum sp.) and plant extract combinations on the growth of mustard plant (Brassica juncea L.) grown in hydroponic wick system. Caraka Tani Journal of Sustainable Agriculture, 37(2), 299-309.
Patil, T. S., Shinde, S. R., & Yadav, R. B. (2023). Novel approach for production of biofertilizer by utilizing fish waste. Journal of Survey in Fisheries Sciences, 10(2), 5–10.
Prasedya, E. S., Kurniawan, N. S. H., Kirana, I. A. P., Ardiana, N., Abidin, A. S., Ilhami, B. T. K., Jupri, A., Widyastuti, S., Sunarpi, H., & Nikmatullah, A. (2022). Seaweed fertilizer prepared by EM-fermentation increases abundance of beneficial soil microbiome in paddy (Oryza sativa L.) during vegetative stage. Fermentation, 8(2), 46. https://doi.org/10.3390/fermentation8020046
Purbajanti, E. D., & Setyawati, S. (2020). Organic fertilizer improve the growth, physiological characters and yield of pak choy. Agrosains: Jurnal Penelitian Agronomi, 22(2), 83-87. https://doi.org/10.20961/agsjpa.v22i2.43112
Putra, Z. A., Dewi, E. N., Purnamayati, L., & Rianingsih, L. (2022). The effect of the addition of protease enzyme on the specification of liquid organic fertilizer Sargassum sp. Saintek Perikanan: Indonesian Journal of Science and Technology, 18(1), 47-52. https://doi.org/10.14710/ijfst.18.1.47-52
Pyakurel, A., Dahal, B. R., & Rijal, S. (2019). Effect of molasses and organic fertilizer in soil fertility and yield of spinach in Khotang, Nepal. International Journal of Applied Sciences and Biotechnology, 7(1), 49–53. https://doi.org/10.3126/ijasbt.v7i1.23301
Raghunandan, B., Vyas, R., Patel, H., & Jhala, Y. (2019). Perspectives of seaweed as organic fertilizer in agriculture. Soil fertility management for sustainable development, 267-289. https://doi.org/10.1007/978-981-13-5904-0_13
Raj, T. S., Nishanthi, P., Graff, K. H., & Suji, H. A. (2018). Seaweed extract as a biostimulant and a pathogen controlling agent in plants. International Journal of Tropical Agriculture, 36(3), 563-580.
Raman, J., Kim, J.-S., Choi, K. R., Eun, H., Yang, D., Ko, Y.-J., & Kim, S.-J. (2022). Application of lactic acid bacteria (LAB) in sustainable agriculture: Advantages and limitations. International Journal of Molecular Sciences, 23(14), 7784. https://doi.org/10.3390/ijms23147784
Rao, G. M. N., & Chatterjee, R. (2014). Effect of seaweed liquid fertilizer from Gracilaria textorii and Hypnea musciformis on seed germination and productivity of some vegetable crops. Universal Journal of Plant Science, 2(7), 115–120. https://doi.org/10.13189/ujps.2014.020701
Sedayu, B., Erawan, I., & Assadad, L. (2014). Liquid fertilizer from Eucheuma cottonii, Sargassum sp, dan Gracilaria sp. using composting process. JPB Perikanan, 9(1), 61-68.
Silitonga, P. R. A., Setyati, W. A., Susanto, A., & Sibero, M. T. (2022). Pengaruh Fermentasi Gracilaria verrucosa dengan Penambahan Starter Lactobacillus plantarum pada Profil Metabolit dan Aktivitas Biologisnya [Effect of Fermentation of Gracilaria verrucosa with Addition of Lactobacillus plantarum Starter on Metabolite Profile and Biological Activity]. Journal of Marine Research, 11(2), 309-319. https://doi.org/10.14710/jmr.v11i2.33262
Suhameena, B., Devi, S., Gowri, R., & Kumar, A. (2020). Utilization of Azospirillum as a Biofertilizer–an overview. Int J Pharm Sci Rev Res, 62(22), 141-145.
Tsaniya, A., Dewi, E., & Anggo, A. (2021). Characteristics of liquid organic fertilizer from different composition types of seaweed between Gracilaria sp. and Sargassum sp. Journal of Physics: Conference Series. https://doi.org/10.1088/1742-6596/1943/1/012071
Uruilal, C., Talahaturuson, A., Rumahlewang, W., & Patty, J. (2017). Isolate Trichoderma spp. and its antagonism to Sclerotium rolfsii sacc. cause of wilt disease in chili (Capsicum anuum) in-vitro. Jurnal Budidaya Pertanian, 13(2), 64-67. https://doi.org/10.30598/jbdp.2017.13.2.64
Wang, P., Souma, K., Okamoto, H., Yano, T., Nakano, M., Furudate, A., Sato, C., Zhang, J., & Masuko, T. (2014). Effects of addition of Lactobacillus plantarum and Enterococcus faecium inoculants to high-nitrogen fertilized timothy (Phleum pratense L.) on fermentation, nutritive value, and feed intake of silage. American Journal of Plant Sciences, 5, 3889–3897.
Zayed, M. S. (2018). Enhancement the feeding value of rice straw as animal fodder through microbial inoculants and physical treatments. International Journal of Recycling of Organic Waste in Agriculture, 7, 117-124. https://doi.org/10.1111/j.1365-2672.1968.tb00367
How to Cite this Article:
Dewi, E.N., A.R. Tsaniya & L. Purnamayati. 2024. The effect of Gracilaria liquid organic fertilizer fermented with different starters on the growth of pakcoy. Jurnal Perikanan Universitas Gadjah Mada. 26 (1): xx-xx. https://doi.org/10.22146/jfs.93277
DOI: https://doi.org/10.22146/jfs.93277
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