* Corresponding Author
PENELUSURAN SENYAWA BIOAKTIF BAKTERI YANG BERASOSIASI DENGAN ANEMON LAUT SERTA IDENTIFIKASI MOLEKULERNYA BERBASIS 16S rDNA
https://doi.org/10.22146/jfs.9049 Naely K Wusqy(1*), Dwi I Prayitmo(2), Ocky K Radjasa(3), Leenawaty Limantara(4), Ferry F Karwur1(5)
(1) Universitas Gadjah Mada
(2) Universitas Kristen Satya Wacana
(3) Fakultas Perikanan dan Ilmu Kelautan Universitas Diponegoro
(4) Fakultas Perikanan dan Ilmu Kelautan Universitas Diponegoro
(5) Fakultas Perikanan dan Ilmu Kelautan Universitas Diponegoro
(*) Corresponding Author
Abstract
Sea anemone-associated microorganisms are among of the most interesting and promising marine natural product sources, which produce various biological activities. In this study, marine bacteria were isolated from 2 kinds of sea anemones, Entacmaea medusivora and Green Anemone that were collected from Bandengan, Jepara and Maratua, East Borneo respectively. Nine bacterial isolatesof E.medusivora were screened for antibacterial activity against 8 strains. One out of 9 bacterial isolates was successfully screened and was found to be active against V. parahaemolyticus strains. This active isolate was also capable of amplifying PKS gene fragments necessary for the biosynthesis of polyketide synthase. The identifi cation results revealed that the active isolate is Bacillus cereus. One bacterial isolate of Green Anemone was screened to produce the pigment and positively synthesize carotenoids. Initial analysis with atomic absorption spectrophotometric method revealed that the wave length of bacterial pigment were in the range of 300-600 nm, which are categorized that within the group of carotenoid pigments. From the results of molecular identifi cation by 16S rDNA method, it was shown that bacterium was closely related to Xanthomonas sp.
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Ansari, M.Z. 1992. NRPS-PKS: A Knowledge-Based Resource for Analysis of NRPS/PKS Megasynthetases.Nucleic Acids Res. 32:405-413.
Atschul, S.F., T.L. Madden, A.A. Schaffer, J. Zhang, Z. Zhang, W. Miller & D.J. Lipman. 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleid Acid Res. 25:3389-3402.
Britton, G., L. Jensen & H. Pfander. 1995.Carotenoid. Birkäuser Verlag Basel. Boston. Berlin.
Bunch, A.W. & R.G. Harris. 1986. The Manipulation of Organism for the production of secondary metabolites. In: Russel, G.G. (ed). Biotechnology and Genetic Engineering Review. Vol 4. Intercept Ltd. New Castle. 117-144 pp.
Burgess, J.G., E.M. Jordan, M. Bregu, A.M.Spragg & K.G. Boyd. 1999. Microbial antagonism: a neglected avenue of natural products research. J. Biotechnol 70:27–32.
Friese, E.U. 1993. Sea Anemones as a Hobby. TFH Publications. New Jersey. 365 p.
Gross, J. 1991. Pigments in Vegetables: chlorophylls and carotenoids. Van Nostrand Reinhold. New York.
Isnansetyo, A. & Y. Kamei. 2003. Pseudoalteromonas phenolica sp.: a novel marine bacterium that produces phenolic anti-methicillin-resistantStaphylococcus aureus substances. Int. J. Syst. Evol. Micr. 53: 583-588.
Istiqomah, I., Triyanto., A. Isnansetyo., H.N. Kamiso & M. Murdjani. 2004. Patogenisitas Vibrio fl uvialis yang diisolasi Dari Kerapu Tikus (Crimileptes altivelis) dalam A. Irianto (Ed) Prosiding Seminar Nasional Penyakit Ikan dan Udang IV. Purwokerto. 155-160.
Jeffrey, S.W., R.F.C. Mantoura & S.W. Wright. 1997. Phytoplankton pigments in oceanography. United Nations Educational. Scientific and Cultural Organization, Paris.
Kopsell, D.A. & D.E. Kopsell. 2006. Accumulation and Bioavaibility of Dietary Carotenoids in Vegetable Crops. Plant Sci. 11: 499-507.
Kuki, M., H. Nagae, R.J. Cogdell, K. Shimada & Y. Koyama. 1994. Solvent Effect on Spheroidene in Non Polar and Polar Solutions and The Environtment of Spheroidene in The Light-Harvesting Complexes of Rhodobacter sphaeroides 2.4.1 as Revealed by Energy of the 1Ag-→1Bu+Absorbstion and
The Frequencies of The Vibronically Coupled C=CStretching Raman Lines in The 1Ag- and 2 Ag- State. Photochem. Photobiol. 59:116-124.
Madigan, M.T., J.M. Martinko & J. Parker. 2000. Brock Biology of Microorganisms. Prentice Hall. New Jersey.
Maeda, H., H. Masashi, S. Tokute, F. Katsura & M. Kazuo. 2005. Fucoxanthin from Edible Seaweed, Undaniria Pinnatifi da, Shows Antiobesity Effect Trough UCP1 Expression in White Adipose Tissues. Biochem. Biophys. Res. Communications
:392-397.
Mahendra, E & H. Semangun. 2008. Karotenoid Dalam Budidaya Udang.Prosiding Back to Nature dengan Pigmen Alami-Seminar Nasional Pigmen 2008, Salatiga 5 September 2008: 386-395.
Manitto, P. 1981. Biosintesis Produk Alami. IKIP Semarang Press. Semarang. 597 hlm (Alih Bahasa : Koensoemardiyah).
Piel, J. 2002. A polyketide synthase-peptide synthetase gene cluster from an uncultured bacterial symbiont of Paederus beetles. Proc. Natl. Acad. Sci. USA. 29: 99(22).
Proksch, P., R.A. Edrada & R. Ebel. 2002. Drugs from the Seas – Current Status and Microbiological Implications. Appl. Microbiol. Biotechnol.59 : 125-124.
Radjasa, O.K. 2005. Eksplorasi Bakteri Laut Penghasil Metabolit Sekunder yang Berasosiasi dengan Karang Lunak Melalui Pendekatan Kultur dan Non Kultur; Usulan Penelitian Hibah Bersaing Perguruan Tinggi. Universitas Diponegoro. Semarang. (Tidak dipublikasikan).
Radjasa, O.K., T. Martens, H.P Grossart, T. Brinkhoff, A. Sabdono & M. Simon. 2007. Antagonistic Activity of a Marine acterium Pesueodaltromonas luteoviolacea TAB 4.2 Associated with Coral Acroporasp. J. Bio. Sci. 7 (2): 239-246.
Shiomi, K., T. Honma, M. Ide, Y.Nagashima, M. Ishida & M. Chino. 2003. An Epidermal Growth Factor-like Toxin and Two Sodium Channel Toxins from the Sea Anemone Stichodactyla gigantea. Toxicon.41: 229-236.
Terkina, I.A., V.V. Parfenova, & T.S. Ahn. 2006. Antagonistic Activity of Actinomycetes of Lake Baikal. Appl. Biochem. Microbiol 2006 42(2):173–176.
Thiel, V & J.F Imhoff. 2003. Phylogenetic Identifi cation of Bacteria with Antimicrobial Activities Isolated from Mediterranean Sponges. Biomol. Engg. 20:421-423.
Zein, U. 2004.Diare Akut Infeksius Pada Dewasa. e-USU RepositoryFakultas Kedokteran Divisi Penyakit Tropik dan Infeksi Bagian Ilmu Penyakit Dalam. Universitas Sumatera Utara. Sumatera Utara.
DOI: https://doi.org/10.22146/jfs.9049
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Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).
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