Isolation and Identification of Aeromonas spp. from Diseased African Catfish (Clarias sp.) in Ngawi Regency

https://doi.org/10.22146/jfs.26917

Sri Rejeki(1), Triyanto Triyanto(2), Murwantoko Murwantoko(3*)

(1) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada.
(2) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada.
(3) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada.
(*) Corresponding Author

Abstract


African catfish (Clarias sp.) is one of important freshwater fish which prefer consumed by people due to many advantages. Aeromonas sp. bacteria are dangerous patogen for freshwater fishes. This objective of the research was to isolate, identify and determine pathogenicity of Aeromonas sp. bacteria from African catfish from District Ngawi. The samples of catfish with 20 - 25 cm length showing clinical signs disease were obtained from three sub districts. Bacteria were isolated from kidney and inoculated into GSP medium. Characterization and identification through morphology of bacterial colonies, cells and biochemical test. Postulate Koch was conducted to verify abaility to couse disease. Pathogenicity was analyzed by determination of value of Lethal Dosage-50 on catfish on 7 - 9 cm length. The result showed that the disease symptoms of fish infected by the bacteria were skin ulcer, abdominal swelling and kidney damage. Fifteen bacterial isolates were collected which five, six and four isolates were from Kecamatan Karang Jati, Geneng dan Paron.sub-district respectively. The result showed 12 isolates (GKJ1, GKJ3, GKJ4, GGN1, GGN2, GGN3, GGN4, GGN5, GGN6, GPR2, GPR3 and GPR4) were identified as Aeromonas hydrophila. Three isolates (GKJ2, GKJ5 and GPR1) were identified as A. salmonicida. Isolate of A. hydrophila GKJ1, GKJ4, GGN2, GGN5, GPR2 and GPR4 were virulent to African catfish with LD50 values of 1,55 x 105, 3,89 x 105, 7,24 x 105, 2,39 x 105, 6,61 x 104 and 1,95 x 104 cfu/fish.

Keywords


Aeromond; african catfish; identification; pathogenicity

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References

Angka, S.L., T.J. Lam & Y.M. Sin. 1995. Some virulence characteristics of Aeromonas hydrophila in walking catfish. Aquacultur 130 : 103-112.

Austin, B. & D.A. Austin. 1987. Bacteria Fish Pathology; Diseases in Farmed and Wild Fish. Ellis Horwood Limited, England.

Ellis, A.E. 1988. Optimizing Factors for Fish Vaccination. In: fish vaccination. A.E. Ellis. (Ed). Academic Press, London : 32-46.

Holt, J.G., N.R. Krieg, P.H.A. Sneath, J.T. Staley & S.T. Williams. 1994. Bergey’s Manual of Determinative Bacteriology. 9th ed. William & Wilkins. Departement of Microbiology, Gltner Hall, Michigan State University, East lansing, MI, USA, 48824-1101.

Hubert, J.J. 1980. Biossay. Kendall/Hunt Publishing Company, Lowa, USA

Irianto, A., 2004. Patologi Ikan Teleostei. Gadjah Mada Univesity Press, Yogyakarta.

Nitimulyo, K.H., A. Sarono., I.Y.B. Lelana., N. Widodo., E.B. Thaib., S. Haryani., Haryanto., Triyanto., Ustadi., A.N. Kusumahati., W. Novianti., S. Wardani & Setianingsih. 1993. Hama dan Penyakit Ikan Golongan Bakteri : Buku 2. Pusat Karantina Pertanian dan Jurusan Perikanan. Fakultas Pertanian. Universitas Gadjah Mada.

Nitimulyo, K.H. 1996. Vibriosis pada ikan dan alternatif penanggulangannya. Jurnal Perikanan I (I) : 78-86.

Macfaddin, J.F. 1980. Biochemical Test for Identification of Medical Bacteria. 2nd ed. Williams & Wilkins, Waverly Press, Inc. Mt. Royal, and Guilferd Aves, Baltimore Md. 21202, USA

Murwantoko, Rozi, I. Istiqomah & K.H. Nitimulyo. 2013. Isolasi, Karakterisasi dan Patogenitas Bakteri Penyebab Penyakit pada Gurami (Osphronemus goramy) di Kabupaten Bantul. Jurnal Perikanan 15:83-90

Noga, E.J. 2000. Fish Disease Diagnosis and Treatment. Iowa State Press, North California.

Stevenson, R.M.W. 1988. Vaccination againt Aeromonas hydrophila. In: fish vaccination. A.E. Ellis. (Ed). Academic Press, London : 112-123.

Supriyadi, H & M. Shariff. 1996. Determinasi virulensi beberapa isolat Aeromonas hydrophila dengan metode Plate Assay dan LD50. Prosiding Simposium Perikanan Indonesia I. Badan Penelitian dan Pengembangan Pertanian. Jakarta : 279-284.

Triyanto. 1990. Patogenesitas beberapa isolat Aeromonas hydrophila terhadap ikan lele (Clarias Batracus L). Prosiding Seminar Nasional II Penyakit Ikan dan Udang 16-18 Januari. Badan Peneliti dan Pengembangan Pertanian : 116-121.

Wakabongo, M., B. Enoch., B. F.A. Meier & H.P. Dalton. 1992. Rapid identification of motil Aeromonas. Diagn Microbiology Infect Dis 15 : 511-515.



DOI: https://doi.org/10.22146/jfs.26917

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Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).

 

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