Peningkatan Respon Imun Non-Spesifik Benih Kerapu Bebek, Cromileptes altivelis dengan Imunostimulan dan Bakterin terhadap Infeksi Viral Nervous Necrosis (VNN)

https://doi.org/10.22146/jfs.160

Des Roza(1), Fris Johnny(2*), Tridjoko Tridjoko(3)

(1) Balai Besar Riset Perikanan Budidaya Laut Gondol
(2) Balai Besar Riset Perikanan Budidaya Laut Gondol
(3) Balai Besar Riset Perikanan Budidaya Laut Gondol
(*) Corresponding Author

Abstract


An experiment to evaluate the effectiveness immunostimulant and bacterin on humpback grouper have been conducted at the Disease Laboratory of Research Institute for Mariculture, Gondol, Bali. The experiment was designed in completely randomized design with four treatments in duplicates. Two hundred of humpback grouper juveniles (15-18 cm of total length, 55-65 g of body weight) were injected intraperitoneally with (A) bacterine at 107 cfu/kg body weight (BW), (B) peptidoglycan at 100 mg/kg BW, and (C) immuno star at 100 ml/kg BW, (D) control. The fish were then challenged with VNN by intramuscular injection at 10 days post treatment. Results showed that survival rates of juvenile after challenged with VNN were 60.00% (B & C), 53.34% (A), and 1.67% (control). Parameters of non-spesific immune respons showed that phagocytic activities were 17.56% (B), 17.55% (C), 13.11% (A), and 9.33% (control). In addition, lisozyme activities were 1.64 cm (B), 1.58 cm (C), 1.55 cm (A), and 1.46 cm (control). Immunostimulant and bacterin stimulated non-specific immune response, and increased survival rate of humpback grouper juvenile.


Keywords


humpback grouper, immunostimulant, non-specific immune response, VNN

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References

Anderson, D.P. 1996. Environmental factors in fish health: immunological aspects. In: The fish immune system: organism, pathogen and environment. G. Iwama and T. Nakanishi (Eds.). Academic Press. California: 289-310.

Arimoto, M., K. Mori, T. Nakai, K. Muroga, and I. Furusawa. 1993. Pathogenicity of the causative agent of viral nervous necrosis disease in striped jack, Pseudocaranx dentex (Bloch and Schneider). J. Fish Disease. 16: 461-469.

Dalmo, R.A. and R. Seljelid. 1995. The immunomodulatory effect of LPS, laminaran and sulphated laminaran { β(1,3)-D-glucan} on Atlantic salmon, Salmo salar L., macrophages in vitro. J. Fish Diseases. 18: 175-185.

Dalmo, R.A., B. Martinsen, T.E. Horsberg, A. Ramstad, C. Syvertsen, R. Seljelid, and K. Ingebrigtsen. 1998. Prophyl-actic effect of β(1,3)-D-glucan (lamina-ran) against experimental Aeromonas salmonicida and Vibrio salmonicida infection. Journal of Fish Disease. 21: 459-462.

Elder, A., A. Horovitcz, and H. Bercovier. 1997. Development and efficacy of a vaccine against Streptococcus iniae infection in farmed rainbow trout. Veterinary Immunology and Immuno-pathology. 56: 175-183.

Ellis, A.E. 1993. Lysozyme assays. In: Techniques in Fish Immunology-1. J.S. Stolen, T.C. Fletcher, D.P. Anderson, B.S. Roberson, and W.B. van Mulswinkel (Eds.). SOS Publications. Fair Haven. NJ 07704-3303. USA: 101-103.

Figueras, A., M.M. Santarem, and B. Novoa. 1998. Influence of the sequence of administration of β-glucans and Vibrio damsela vaccine on the immune response of turbot (Scophthalmus maximus L.). Veterinary Immunology and Immuno-pathology. 64: 59-68.

Itami, T., M. Kando, M. Uozu, A. Suganuma, T. Abe, A. Nakagawa, N. Suzuki, and Y. Takahashi. 1996. Enhancement of resistance against Enterococcus seriolicida infection in yellowtail, Seriola quinqueradiata (Temminck and Schlegel), by oral administration of peptidoglycan derived from Bifidobacterium thermo-phylum. J. Fish Diseases. 19: 185-187.

Johnny, F., I. Koesharyani, D. Roza, Tridjoko, N.A. Giri, dan K. Suwirya. 2001. Respon ikan kerapu bebek, Cromileptes altivelis terhadap imuno-stimulan peptidoglycan melalui pakan pelet. J. Penel. Perikanan Indonesia. VII (4): 52-56.

Johnny, F. dan D. Roza. 2002. Pengaruh penyuntikan imunostimulan peptido-glikan terhadap peningkatan tanggap kebal non-spesifik ikan kerapu macan, Epinephelus fuscoguttatus. Laporan Penelitian Balai Besar Riset Perikanan Budidaya Laut. Gondol. 12 p.

Jorgensen, J.B., H. Lunde, and B. Robertsen. 1993. Peritoneal and head kidney cell response to intra-peritoneally injected yeast glucan in Atlantic salmon, Salmo salar L. J. Fish Disease. 16: 313-325.

Kawakami, H., N. Shinohara, and M. Sakai. 1998. The non-specific immu-nostimulation and adjuvant effects of Vibrio anguillarum bacterin, M-glucan, chitin and Freud’s complete adjuvant against Pasteurella piscicida infection in yellowtail. Fish Pathology. 33 (4): 287-292.

Klontz, G.W. 1997. Fish Hematology. In: Techniques in Fish Immunology-3 (2nd Ed). Stolen, J.S., T.C. Fletcher, A.F. Rowley, J.T. Zelikoff, S.L. Kaattari, S.A. Smith. (Eds.). SOS Publications, Fair Haven, NJ 07704-3303. USA: 121-131.

Koesharyani, I., Zafran, dan K. Yuasa. 1999. Deteksi viral nervous necrosis (VNN) menggunakan polymerase chain reaction (PCR) pada ikan kerapu bebek, Cromileptes altivelis. Prosiding Seminar Nasional Penelitian dan Desiminasi Teknologi Budidaya Laut dan Pantai. Jakarta. 12 p.

Koesharyani, I., D. Roza, K. Mahardika, F. Johnny, Zafran, and K. Yuasa. 2001. Marine fish and crustaceans diseases in Indonesia In: Manual for fish diseases diagnosis II. K. Sugama, K. Hatai and T. Nakai. (Eds.). Gondol Research Station for Coastal Fisheries, CRIFI and Japan International Co-operation Agency. 49 p.

Matsuo, K. and I. Miyazono. 1993. The influence of long-term administration of peptidoglycan on disease resistance and growth of juvenile rainbrow trout. Nippon Suisan Gakkaishi. 59 (8): 1377-1379.

Matsuyama, H., R.E.P. Mangindaan, and T. Yano. 1992. Protective effect of schizophyllan and scleroglucan against Streptococcus sp. infection in yellowtail (Seriola quinqueradiata). Aquaculture. 101: 197-203.

Mori, K., K. Mushiake, and M. Arimoto. 1998. Control measures for viral nervous necrosis in striped jack. Fish Pathology. 33 (4): 443-444.

Mushiake, K., T. Nishizawa, T. Nakai, I. Furusawa, and K. Muroga. 1994. Control of VNN in striped jack; Selection of spawners based on the detection of SJNNV gene by polymerase chain reaction (PCR). Fish Pathology. 29 (3): 93-98.

Nakai, T., H.D. Nguyen, T. Nishizawa, K. Muroga, M. Arimoto, and Otsuki. 1994. Outbreaks of viral nervous necrosis in Epinephelus moara and tiger puffer Takifugu rubripes. Fish Pathology. 29: 211-212.

Nishizawa, T., K. Mori, T. Nakai, I. Furusawa, and K. Muroga. 1994. Polymerase chain reaction (PCR) amplication of RNA of striped jack nervous necrosis virus (SJNNV). Dis. Aquat. Org. 18: 103-107.

Raa, J., G. Roerstad, R. Engstad, and B. Robertsen. 1992. The use of immunostimulants to increase resistance of aquatic organisms to microbial infections. In: Diseases in Asian aquaculture. I.M. Shariff, R.P. Subasinghe, and J.R. Arthur (Eds.). Fish Health Section. Asian Fisheries Society. Manila. Philippines: 39-50.

Rowley, A.F. 1993. Collection, separa-tion and identification of fish leuco-cytes. In: Techniques in fish immuno-logy-1. Stolen, J.S., T.C. Fletcher, D.P. Anderson, B.S. Roberson, W.B. van Mulswinkel. (Eds.). SOS Publications. Fair Haven. NJ 07704-3303. USA: 114-136.

Roza, D., F. Johnny, S. Kawahara, dan A. Hanafi. 2002a. Penyakit pada budidaya ikan kerapu dan upaya penanggu-langannya. Kumpulan Makalah Seminar Pengembangan Teknologi Budidaya Kerapu. Balai Budidaya Laut Lampung-JICA: 75-86.

Roza, D., K. Mahardika, F. Johnny, Zafran, dan Tridjoko. 2002b. Pengaruh per-bedaan dosis vaksin melalui perendam-an terhadap ketahanan juvenil kerapu bebek, Cromileptes altivelis oleh infeksi Viral Nervous Necrosis (VNN). Laporan Hasil Penelitian DIP 2002. Balai Besar Riset Perikanan Budidaya Laut Gondol: 91-101.

Roza, D., K. Mahardika, F. Johnny, Zafran, dan Tridjoko. 2002c. Pengaruh lama perendaman dalam vaksin terhadap ketahanan junevil kerapu bebek, Cromileptes altivelis oleh infeksi Viral Nervous Necrosis (VNN). Laporan Hasil Penelitian DIP 2002. Balai Besar Riset Perikanan Budidaya Laut Gondol. 102-111.

Roza, D., F. Johnny, and K. Mahardika. 2003. Viral diseases of grouper in Indonesia. Makalah pada Training on Grouper Hatchery Seed Production. Balai Besar Riset Perikanan Budidaya Laut Gondol.- NACA Bangkok. Gondol 1-21 Mei 2003. 12 p.

Rukyani, A., E. Silvia, A. Sunarto, dan Taukhid. 1997. Peningkatan respon kebal non-spesifik pada ikan lele dumbo (Clarias sp) dengan pemberian imuno-stimulan ( β-glucan). JPPI II (1): 1-10.

Santarem, M., B. Novoa, and A. Figueras. 1997. Effect of β-Glucan on the non-specific immune responses of turbot (Schopthalamus maximus L.). Fish and Sellfish Immunology. 7: 429-437.

Secombes, C.J. 1996. The nonspecific immune system; cellular defences. In: The fish immune system: organism, pathogen and environment. G. Iwama and T. Nakanishi (Eds.). Academic Press. San Diego. California: 63-95.

Siwicki, A.K. and D.P. Anderson. 1993. Immunostimulation in fish; measures the effects of stimulants by serological and immunological methods, Internati-onal Workshop and Training Course in Poland. 15 p.

Thompson, K.D., J.H. Lilley, S.C. Chen, A. Adams, and R.H. Richards. 1999. The immune response of rainbow trout (Oncorhynchus mykiss) against Aphanomyces invadans. Fish and Shellfish Immunology. 9: 195-210.

Vadstein, O. 1997. The use of immuno-stimulan in marine larviculture: possibi-lities and challenges. Aquaculture. 155: 401-417.

Watanabe, K., S. Suzuki, T. Nishizawa, K. Suzuki, M. Yoshimizu, and Y. Ezura. 1998. Control strategy for viral nervous necrosis of barfin flounder. Fish Pathology. 33 (4): 445-446.

Yuasa, K., I. Koesharyani, D. Roza, F. Johnny, and Zafran. 2001. Manual for PCR procedure; Rapid diagnosis on Viral Nervous Necrosis (VNN) in grouper. Lolitkanta-JICA Booklet No. 13. 35 p.

Zafran, I. Koesharyani, D. Roza, F. Johnny, dan K. Yuasa. 1998. Peningkatan sintasan ikan kerapu tikus (Cromileptes altivelis) dengan penambahan vitamin dan imuno-stimulan ke dalam pakan segar. Sudrajat, A., E.S. Heruwati, K. Sugama, A. Purnomo, Z.I. Azwar, dan I.N.A. Giri. (Eds.) Seminar Teknologi Perikanan Pantai. Denpasar 6-7 Agustus 1998. 164-168.

Zafran, I. Koesharyani, F. Johnny, dan Wardoyo. 1999. Pengaruh imuno-stimulan peptidoglycan terhadap keragaan juvenil ikan kerapu bebek, Cromileptes altivelis. Loka Penelitian Perikanan Pantai Gondol-Bali. Lapor-an Penelitian. 6 p.



DOI: https://doi.org/10.22146/jfs.160

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Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).

 

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