Viabilitas Sperma Ikan Nilem (Osteochilus hasselti C.V.) Setelah Penyimpanan Jangka Pendek dalam Larutan Ringer
Gratiana E. Wijayanti(1*), Sorta B.I. Simanjuntak(2)
(1) Lab. Struktur dan Perkembangan Hewan Fakultas Biologi UNSOED
(2) Lab Fisiologi Hewan Fakultas Biologi UNSOED
(*) Corresponding Author
Abstract
assessed by its motility, progressivity and ability to fertilize fresh eggs. The results showed that the sperm were immotile in either undiluted or diluted in Ringer solution. Upon activation by diluting in water, the sperm of the control groups as well as the tested groups showed a high motility (>70%) and highly progressive (>3). The sperm were capable to fertilize eggs as indicated by a high fertilization rate of more than 90%. The highest hatching rate (HR) was produced by the control groups while HR of milt stored for 3, 6 and 9 days were 60%, 1% and 0%, respectively. Low HR produced by milt stored for 6 and 9 days was resulted from a high proportion of abnormal embryos which led to their mortality around gastrula stage. It could be concluded that the viability of the nilem’s sperm could be diluted in Ringer solution and kept in the refrigerator as long as for 3 days.
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DOI: https://doi.org/10.22146/jfs.142
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Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).
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