Interaksi Gen pada Beberapa Karakter Kualitatif Tomat (Solanum lycopersicum L.)

https://doi.org/10.22146/veg.67540

Arya Widura Ritonga(1*), Siti Marwiyah(2), Erin Puspitarini(3), Muhamad Syukur(4)

(1) Departemen Agronomy dan Hortikultura Fakultas Pertanian Institut Pertanian Bogor
(2) Departemen Agronomy dan Hortikultura Fakultas Pertanian Institut Pertanian Bogor
(3) 
(4) 
(*) Corresponding Author

Abstract


Program pemuliaan tanaman dapat lebih efektif dan efisien jika terdapat informasi pewarisan sifat dari karakter yang akan dimuliakan. Namun, belum semua karakter kualitatif pada buah tomat diketahui pola pewarisan sifatnya. Penelitian ini bertujuan untuk memperoleh informasi tentang pewarisan sifat beberapa karakter kualitatif pada tanaman tomat. Penelitian ini dilakukan pada Januari – April 2020 di Kebun Percobaan IPB Leuwikopo, Bogor, Indonesia. Sebanyak 10 tanaman varietas Tora IPB, 10 tanaman genotype GIK serta 10 tanaman F1 (hasil persilangan Tora IPB x GIK) digunakan pada penelitian ini. Selain itu, juga digunakan sebanyak 185 tanaman populasi F2 (hasil selfing F1 Tora IPB x GIK) pada penelitian ini. Analisis data dilakukan menggunakan uji khi kuadrat dilakukan pada populasi F2 untuk menentukan rasio segregasi karakter kualitatif yang paling tepat. Hasil pengamatan menunjukkan bahwa karakter posisi anak daun terhadap tangkai daun, bentuk ujung buah tomat, tinggi kelopak terhadap mahkota, dan karakter luas plasenta buah tomat dikendalikan oleh 2 gen dengan 2 alel per lokus. Tipe intraksi gen pada pewarisan karakter posisi anak daun terhadap tangkai daun dan bentuk ujung buah tomat yaitu epistasis dominan, sedangkan tipe interaksi gen pada pewarisan karakter tinggi kelopak terhadap mahkota dan karakter luas plasenta buah tomat berturut-turut adalah epistasis dominan ganda dan semi epistasis. Karakter green shoulder pada buah tomat dikendalikan oleh 1 gen dengan 2 alel per lokus. Gen pengendali karakter green shoulder bersifat dominan terhadap karakter tanpa green shoulder (polos) pada buah tomat.


Keywords


dominan; interaksi gen; pewarisan sifat; resesif; segregasi.

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References

[FAO] Food and Agricultural Organization. 2021. Statistic crop production. http://faostat3.fao.org. [7 Juli 2021].

[Kementan] Kementerian Pertanian. 2021. Basis data statistik pertanian di Indonesia. http://aplikasi.pertanian.go.id. [7 Juli 2021].

[UPOV]. 2011. Guidelines for The Conduct of Test for Distinctness, Uniformity and Stability for Tomato. Geneva: International Union for The Protection of New Varieties of Plants.

Ahmad, M., A. Kanwal, M. Iqbal, B.A. Khan, M. Shahid, A. Rehman, F. Khan, I. Ullah and I. Hussain. 2019. Homozygosity and segregation ratio in F4 generation of tomato for fruit morphology. MOJ Ecology and Environmental Science. 4(6):259-261.

Amangous, N.F., K.K. Koffi, J.P. Baudoin and I.A.Z. Bi. 2016. Inheritance of fruit neck, rind and seed coat hardness, and seed coat colour. South African Journal of Plant and Soil. 2018:1-8.

Anas, A. & I.L. Hakim. 2017. Pola pewarisan karakter umur tanaman sorgum (Sorghum bicolor (L.) Moench). Jurnal Agrikultura. 28(2):103-110.

Arcos, M.G., M.E.N. Fonseca, D.B. Zandonadi, L.E.P. Peres, A. Arruabarrena, D.S. Ferreira, Z. Kevei, F. Mohareb, A.J. Thompson and L.S. Boiteux. 2019. A loss-of-function allele of a TAC1-like gene (SITAC1) located on tomato chromosome 10 is a candidate for the Erectoid leaf (Erl) mutation. Euphytica. 2015:95-211.

Czerednik, A., M. Busscher, G.C. Angenent and R.A. de Maagd. 2015. The cell size distribution of tomato fruit can be changed by overexpression of CDKA1. Plant Biotechnology Journal. 13:259-268.

Hapsari, R., D. Indradewa dan E. Ambarwati, 2017. Pengaruh pengurangan jumlah cabang dan jumlah buah terhadap pertumbuhan dan hasil tomat (Solanum lycopersicum L.). Vegetalika. 6(3):37-49.

Hapshoh, S., M. Syukur, Y. Wahyu dan Widodo. 2016. Pewarisan karakter kualitatif cabai hias hasil persilangan cabai besar dan cabai keriting. Jurnal Agronomi Indonesia. 44(3):286-291.

Lazaro, A. 2018. Tomato landrace: an analysis of diversity and preferences. Plant Genetic Resources. 16(4):315-324. https://doi.org/10.1017/S1479262117000351

Li, F., X. Song, L. Wu, H. Chen, Y. Liang and Y. Zhang. 2018. Heredities on fruit color and pigment content between green and purple fruits in tomato. Scientia Horticulturae. 235:391-396.

Lin, T., J. Li and Q.Huang. 2013. Inheritance of cherry tomato fruit color. Fujian Journal Agricultural Science. 28:987–992. http://dx.doi.org/10.3969/j.issn.1008-0384.2013.10.012.

Mahfud & R.H. Murti. 2020. Inheritance pattern of fruit color and shape in multi-pistil and purple tomato crossing. Agrivita. 42(3):572-583.

Meitei, K.M., G.C. Bora, S.J. Singh and A.K. Sinha. 2014. Morphology based genetic variability analysis and identification of importance characters for tomato (Solanum lycopersicum L.) crop improvement. American-Eurasian Journal Agriultural. and Environmental. Science. 14(10):1105-1111.

Mohamed, S.M., E.E. Ali and T.Y. Mohamed. 2012. Study of heritability and genetic variability among different plant and fruit characters of tomato (Solanum lycopersicum L.). International Joiurnal of Scientific & Technology Research. 1(2):55-58.

Mustafa, M., M. Syukur, S.H. Sutjahjo dan Sobir. 2016. Pewarisan karakter kualitatif dan kuantitatif pada hipokotil dan kotiledon tomat (Solanum lycopersicum L.) silangan IPB T64 x IPB T3. Jurnal Hortikultura Indonesia. 7(3):155-164.

Nadakuduti, S.S., W.L. Holdsworth, C.L. Klein and C.S. Barry. 2014. KNOX genes influence a gradient of fruit chloroplast development through regulation of GOLDEN2-LIKE expression in tomato. The Plant Journal. 78:1022-1033.

Nguyen, C.V., J.T. Vrebalov, N.E. Gapper, Y. Zheng, S. Zhong, Z. Fei and J.J. Giovannoi. 2014. Tomato GOLDEN2-LIKE transcription factors reveal molecular gradients that function during fruit development and ripening. The Plant Cell. 26:585-601.

Powell, A.L.T., C.V. Nguyen, T. Hill, K.L. Cheng, R.F. Balderas, H. Aktas, H. Ashrafi, C. Pons, R.F. Munoz, A. Vicente, J.L. Baltazar, S.C.S. Barry, Y. Liu, R. Chetelat, A. Granell, AV., Deynze, J.J. Giovannoni and A.B. Bennett. 2012. Uniform ripening encodes a golden 2-like transcription factor regulating tomato fruit chloroplast development. Science. 336:1711-1716.

Rani, K.R. & V. Anitha. 2011. Studies on variability, heritability and genetic advance in tomato (Lycopersicon esculentum Mill. International of Bio-resource and Stress Management. 2(4):382-284.

Reddy, K.K.C., S.K. Jain, A.M.B. Kumar, G.S. Krishnan, A.K. Singh and Z. Hussain. 2017. Morphological markers for identification of hybrids and their parental lines in tomato (Solanum lycopersicum). Indian Journal of Agricultural Sciences. 87(5):694-699.

Ritonga, A.W., M. Syukur, R. Yunianti dan Sobir. 2017. Pewarisan sifat beberapa karakter kualitatif dan kuantitatif pada hipokotil dan kotiledon cabai (Capsicum annuum L.). Jurnal Agronomi Indonesia. 45(1):49-55.

Ritonga, A.W., M.A. Chozin, M. Syukur, A. Maharijaya dan Sobir. 2019. Heritabilitas, korelasi dan sidik lintas berbagai karakter tomat pada kondisi naungan dan tanpa naungan. Jurnal Hortikultura Indonesia. 10(2):85-93.

Saleem, M.Y., Q. Iqbal and M. Asghar 2013. Genetic variability, heritability, character association and path analysis in F1 hybrids of tomato. Pakistan Journal of Agricultural Science. 50(4):649-653.

Saputra, H.E., M. Syukur and S.I. Aisyah, 2014. Pendugaan daya gabung dan heritabilitas komponen hasil tomat pada persilangan dialel penuh. Jurnal Agronomi Indonesia. 42(3):203-209.

Wang, Y., J.P. Clevenger, E.I. Berenguer, T. Meulia, E. van de Knaap and L. Sun. 2019. Acomparison of sun, ovate, fs8.1 and auxin application on tomato fruit shape and gene expression. Plant Cell Physiology. 60(5):1067-1081.

Wu, S., J.P. Clevenger, L. Sun, S. Visa, Y. Kamiya and Y. Jikumaru. 2015. The control of tomato fruit elongation orchestrated by sun, ovate and fs8.1 in a wild relative of tomato. Plant Science. 238:95–104.

Wulandari, E.T., M. Syukur dan A. Maharijaya. 2018. Pewarisan karakter hortikultura persilangan Syakira IPB x IPB C320 dalam rangkan merakit varietas unggul cabai hias. Community Horticulturae Journal. 2(1):57-65.

Xie, q., Z. Hu, Z. Zhu, T. Dong, Z. Zhoo, B. Cui and G. Chen. 2014. Overexpression of a novel MADS-box gene SIFYFL delays senescence, fruit ripening and abscission in tomato. Scientific Reports. 4:4367-4377.

Zhang, J., Z. Hu, Y. Wang, X. Yu, C. Liao and M. Zhu, 2018. Suppression of tomato SEPALLATA MADS-box gene, SICMB1, generates altered inflorescence architecture and enlarged sepals. Plant Science. 272:75-87.

Zhang, X., R. Li, L. Chen, S. Niu, Q. Li, K. Xu, J. Wen, C. Ma, J. Tu, T. Fu and J. Shen 2018b. Inheritance and gen mapping of the white flower trait in Brassica juncea. Molecular Breeding. 38:20-29.

Zhang, J., Y. Wang, M. Naeem, M. Zhu, J. Li, X. Yu, Z. Hu and G. Chen, 2019. An AGAMOUS MADS-box protein, SIMBP3, regulates the speed of placenta liquefaction and controls seed formation in tomato. Journal of Experimental Botany. 70(3):909-924.



DOI: https://doi.org/10.22146/veg.67540

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