ANTIFUNGAL ACTIVITY OF TEA TREE ESSENTIAL OIL (Melaleuca alternifolia) AGAINST Trichophyton mentagrophytes

https://doi.org/10.22146/ijvs.v2i1.82689

Andria Meidina Nurshadrina(1), Alya Zahra Firdani(2), Aria Ika Septana(3*), Agustina Dwi Wijayanti(4)

(1) Universitas Gadjah Mada
(2) Universitas Gadjah Mada
(3) Universitas Gadjah Mada
(4) Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Dermatophytosis is a superficial skin disease in animals that affects hair follicles, nails, and keratin tissues caused by dermatophytes. Trichophyton mentagrophytes is one of the zoophilic dermatophyte genera. The conducting of commercial drug therapy has the potential to induce resistance, systemic toxicity, and teratogenic effects that need to be looked out for. Tea tree essential oil (Melaleuca alternifolia) is an alternative in preventing dermatophyte growth due to the presence of a main antifungal component, which is terpinene. This research aims to understand the antifungal activities of tea tree essential oil against dermatophytosis caused by T. mentagrophytes through a zone of inhibition test. Determination of the inhibition zone diameter of tea tree essential oil consisted of various concentrations 5, 10, 20, 40, 60, 80, and 100% by well diffusion method which utilizes Sabouraud Dextrose Agar (SDA). The zone of inhibition diameter is measured every 24, 48, and 72 hours using a vernier scale. The measurement results were calculated statistically. The statistical data analysis result shows a difference in the zone of inhibition between concentrations with a significance level of <0,05. The zone of inhibition diameter corresponds to the increase in tea tree essential oil concentration. The results of the average zone of inhibition at 72 hours incubation time exhibit a decrease in the zone of inhibition diameter when compared to the results of 48-hour and 24-hour incubations. These results present that tea tree essential oil has in vitro antifungal potential against T. mentagrophytes.

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DOI: https://doi.org/10.22146/ijvs.v2i1.82689

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