The Impact of Blood Cells Component to The Drug Binding of Enrofloxacin in Dogs

Abstract

The research had deducted to evaluate the others components of blood cells that may contribute in drug interaction besides the protein plasma. As known that albumin or globulin of plasma played the majority role of functions in drug binding, some results of many studies still need explanation about the impact of other cells components of blood that may involved in the drug binding. These research used the whole blood of 10 patients of dogs that severed from deaseases and 5 samples of healthy dogs. The therapeutic doses of enrofloxacine (10 mg/kg body weight) were injected an hour before the blood sampling through cephalica vein. The whole blood were analyzed to measure the count of red blood cell (RBC), haemoglobin (Hb), white blood cell (WBC), and platelet by impedance, fluorescent flow and hydrodynamic focusing methods, respectively. Enrofloxacin level was analyzed with high performance liquid chromatography (HPLC) method. The calculation results with regression analysis showed that the values of RBC, Hb, WBC, and platelet of sick dogs had no significant impact to the enrofloxacin level (P>0.05). Except the platelet, the blood components of sick dogs were significantly different compare to the healthy dogs (P<0.1 and P<0.05). The enrofloxacin level of all sick dogs were in therapeutic level (1.22±0.11µg/mL) for some pathogenic bacterias.

Keywords : enrofloxacin, blood components, drug binding