CDHA Ceramic Microspheres for Periodontitis Treatment: Synthesis, Characterization and Doxycycline Release Profiles
Sunita Prem Victor(1*), TS Sampath Kumar(2)
(1) 
(2) 
(*) Corresponding Author
Abstract
The present study is focused on the development of doxycycline loaded calcium–deficient hydroxyapatite (CDHA) microspheres for the treatment of periodontitis. The CDHA microspheres were formed by liquid immiscibility effect using gelatin and paraffin oil with varying Ca/P ratios using calcium hydroxide and diammonium hydrogen orthophosphate as precursors. The morphology of the microspheres as characterized by SEM was optimized by varying the gelatin content. The doxycycline incorporation and its release profiles were studied by UV-Visible spectroscopy in phosphate buffer at physiological conditions. The pH of the buffer solution was initially optimized to have maximum amount of drug loading. Doxycycline loading around the physiological pH of 7 has the highest amount of drug incorporation. All the microspheres exhibit similar release profiles with an initial gradual increase reaching a maximum value and then nearly constant release. The microspheres formed using 6% gelatin shows maximum amount of drug release of 80%.
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Di Silvio L, Bonfield W. 1999. Biodegradable drug delivery system for the treatment of bone infection and repair. J Mater Sci: Mater Med 10:653–8.
Mansho I, Wenyi Y, Takaaki A, Akira O, Naoki K.1998. Synthesis of antibioticloaded interporous hydroxyapatite blocks by vacuum method and invitro release testing. Biomaterial 19:817-9.
Alkhraisat MH, Rueda C, Cabrejos-Azama J, Lucas-Aparicio J, Mariño FT,García-Denche JT, Jerez LB, Gbureck U, Cabarcos EL. 2010. Loading and release of doxycycline hyclate from strontium-substituted calciumphosphate cement. Acta Biomateralia 6:1522-8.
Thomazeau H, Langlais F. 1997. Antibiotic release by tricalcium phosphate bone implantation, in vitro and in vivo pharmacokinetics of different galenic forms. Chirurgie 121:663-6.
Yamamura K, Iwata H, Yotsuyanagi T. 1992. Synthesis of antibiotic-loaded hydroxyapatite beads and in vitro drug release testing. J Biomed Mater Res 26:1053-64
Siddharthan A, Seshadri SK, SampathKumar TS. 2004. Microwave accelerated synthesis of nanosized calcium deficient hydroxyapatite. J Mater Sci: Mater Med 15: 1279-84
Bromberg LE, Debra KB, Phillip MF. 2001. Novel periodontal drug delivery system fortreatment of periodontitis. Journal of Controlled Release 71: 251–9
Sunita PV, Sampath Kumar TS. 2008. BCP ceramic microspheres as drug delivery carriers—synthesis, characterization and doxycycline release. J Mater Sci: Mater Med 19: 283-90.
Elena M, Alexandre MR, Nilce CCR, Gloria AS, Josin CM, Gustavo TM. 2003. Dissolution of calcium-deficient hydroxyapatite synthesized at different conditions. Materials Characterization
:203-7.
Monteiro MM, Rocha NCC, Rossi AM, Soares GA. 2003. Dissolution properties of calcium phosphate granules with different compositions in simulated bodyfluid. J Biomed Mater Res A 65:299–305
DOI: https://doi.org/10.22146/theindjdentres.9993
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