Application of  Nannochloris  sp. for Landfill Leachate Biotreatment and Lipids Production

Ildefonso Baldiris-Navarro; Jorge Sanchez; Martha Torres Virviescas; Alvaro Realpe-Jimenez; Juan Fajardo-Cuadro

doi:10.22146/ijc.82483

Application of Nannochloris sp. for Landfill Leachate Biotreatment and Lipids Production

https://doi.org/10.22146/ijc.82483

Ildefonso Baldiris-Navarro^(1*), Jorge Sanchez⁽²⁾, Martha Torres Virviescas⁽³⁾, Alvaro Realpe-Jimenez⁽⁴⁾, Juan Fajardo-Cuadro⁽⁵⁾

(1) Chemical Engineering Program, Universidad de Cartagena, Cartagena 130015, Colombia
(2) Environmental Public Establishment of Cartagena, Cartagena 130015, Colombia
(3) Marine Science Program, Universidad del Sinú, GIDEAM Group, Cartagena 130015, Colombia
(4) Chemical Engineering Program, Universidad de Cartagena, Cartagena 130015, Colombia
(5) Mechanical Engineering Program, Universidad Tecnológica de Bolívar, EOLITO Group, Cartagena 130015, Colombia
(*) Corresponding Author

Abstract

The sparse treatment of highly toxic leachates produced in landfills due to the excessive generation of urban solid waste is a common problem worldwide. For this reason, this research aims to show the convenience of the use of algal biotechnology in leachate bioremediation processes. Nannochloris sp. species was used in this research. It was isolated and cultured for bioassays. The leachate was diluted to 5 and 10% in the microalgae cultures during a period of 8 d in which the growth of the species. Then removal of nutrients (phosphate and nitrate) and the production of lipids by the microalgae were measured. Nannochloris sp. removed more than 70% of the phosphates and 60% of the nitrates from samples. This result shows the benefits of using these microalgae to treat landfill leachate at low cost and also with the potential to obtain bio-lipids that may be useful for biodiesel production.

Keywords

bioremoval; bio-lipids; landfill leachate; microalgae; Nannochloris sp.

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References

[1] Chang, H., Quan, X., Zhong, N., Zhang, Z., Lu, C., Li, G., Cheng, Z., and Yang, L., 2018, High-efficiency nutrients reclamation from landfill leachate by microalgae Chlorella vulgaris in membrane photobioreactor for bio-lipid production, Bioresour. Technol., 266 (5), 374–381.

[2] Chang, H., Feng, H., Wang, R., Zhang, X., Wang, J., Li, C., Zhang, Y., Li, L., and Ho, S., 2023, Enhanced energy recovery from landfill leachate by linking light and dark bio-reactions: Underlying synergistic effects of dual microalgal interaction, Water Res., 231, 119578.

[3] Putra, R.S., and Hastika, F.Y., 2018, Removal of heavy metals from leachate using electro-assisted phytoremediation (EAPR) and up-take by water hyacinth (Eichornia crassipes), Indones. J. Chem., 18 (2), 306–312.

[4] Viegas, C., Nobre, C., Mota, A., Vilarinho, C., Gouveia, L., and Gonçalves, M., 2021, A circular approach for landfill leachate treatment: Chemical precipitation with biomass ash followed by bioremediation through microalgae, J. Environ. Chem. Eng., 9 (3), 150–163.

[5] Tang, C., Gao, X., Hu, D., Dai, D., Qv, M., Liu, D., and Zhu, L., 2023, Nutrient removal and lipid production by the co-cultivation of Chlorella vulgaris and Scenedesmus dimorphus in landfill leachate diluted with recycled harvesting water, Bioresour. Technol., 369, 128496.

[6] Nair, A.T., Senthilnathan, J., and Nagendra, S.M.S., 2019, Application of the phycoremediation process for tertiary treatment of landfill leachate and carbon dioxide mitigation, J. Water Process Eng., 28, 322–330.

[7] Tagliaferro, G.V., Filho, H.J.I., Chandel, A.K., da Silva, S.S., Silva, M.B., and dos Santos, J.C., 2019, Continuous cultivation of Chlorella minutissima 26a in landfill leachate-based medium using concentric tube airlift photobioreactor, Algal Res., 41, 101549.

[8] Liu, X., Chen, G., Tao, Y., and Wang, J., 2020, Application of effluent from WWTP in cultivation of four microalgae for nutrients removal and lipid production under the supply of CO₂, Renewable Energy, 149, 708–715.

[9] Lakshmikandan, M., Murugesan, A.G., Wang, S., Abomohra, A.E.F., Jovita, P.A., and Kiruthiga, S., 2020, Sustainable biomass production under CO₂ conditions and effective wet microalgae lipid extraction for biodiesel production, J. Cleaner Prod., 247, 119398.

[10] Quan, X., Hu, R., Chang, H., Tang, X., Huang, X., Cheng, C., Zhong, N., and Yang, L., 2020, Enhancing microalgae growth and landfill leachate treatment through ozonization, J. Cleaner Prod., 248, 119182.

[11] Vyas, S., Patel, A., Nabil Risse, E., Krikigianni, E., Rova, U., Christakopoulos, P., and Matsakas, L., 2022, Biosynthesis of microalgal lipids, proteins, lutein, and carbohydrates using fish farming wastewater and forest biomass under photoautotrophic and heterotrophic cultivation, Bioresour. Technol., 359, 127494.

[12] Paiva, A.L.P., Gonçalves da Fonseca Silva, D., and Couto, E., 2021, Recycling of landfill leachate nutrients from microalgae and potential applications for biomass valorization, J. Environ. Chem. Eng., 9 (5), 105952.

[13] Scarponi, P, Izzo, F.C., Bravi, M., and Cavinato, C., 2021, C vulgaris growth batch tests using winery waste digestate as promising raw material for biodiesel and stearin production, Waste Manage., 136, 266–272.

[14] Hazman, N.A.S., Mohd Yasin, N.H., Takriff, M.S., Abu Hasan, H., Kamarudin, K.F., and Mohd Hakimi, N.I.N., 2018, Integrated palm oil mill effluent treatment and CO₂ sequestration by microalgae, Sains Malays., 47 (7), 1455–1464.

[15] Purba, L.D.A., Othman, F.S., Yuzir, A., Mohamad, S.E., Iwamoto, K., Abdullah, N., Shimizu, K., and Hermana, J., 2022, Enhanced cultivation and lipid production of isolated microalgae strains using municipal wastewater, Environ. Technol. Innovation, 27, 102444.

[16] Edmundson, S.J., and Wilkie, A.C., 2013, Landfill leachate–a water and nutrient resource for algae-based biofuels, Environ. Technol., 34 (13-14), 1849–1857.

[17] Malakar, B., Das, D., and Mohanty, K., 2023, Utilization of Chlorella biomass grown in waste peels-based substrate for simultaneous production of biofuel and value-added products under microalgal biorefinery approach, Waste Biomass Valorization, 2023, s12649-023-02058-y.

[18] Eldiehy, K.S.H., Daimary, N., Borah, D., Mandal, M., and Deka, D., 2023, Biodiesel production from Chlorella homosphaera by two-step catalytic conversion using waste radish leaves as a source for heterogeneous catalyst, Appl. Biochem. Biotechnol., 195 (7), 4347–4367.

[19] Baldiris-Navarro, I., and Aponte, J.S., 2020, Growth optimization of Chlorella vulgaris in mixotrophic culture enriched with nutrients using experimental design, IOP Conf. Ser.: Mater. Sci. Eng., 844 (1), 012025.

[20] Baird, R., and Bridgewater, L., 2017, Standard Methods for the Examination of Water and Wastewater, 23^rd Ed., American Public Health Association, Washington, DC, US.

[21] Bligh, E.G., and Dyer, W.J., 1959, A rapid method of total lipid extraction and purification, Can. J. Biochem. Physiol., 37 (8), 911–917.

[22] Hu, D., Zhang, J., Chu, R., Yin, Z., Hu, J., Nugroho, Y.K., Li, Z., and Zhu, L., 2021, Microalgae Chlorella vulgaris and Scenedesmus dimorphus co-cultivation with landfill leachate for pollutant removal and lipid production, Bioresour. Technol., 342, 126003.

[23] Tighiri, H.O., and Erkurt, E.A., 2019, Biotreatment of landfill leachate by microalgae-bacteria consortium in sequencing batch mode and product utilization, Bioresour Technol., 286, 121396.

[24] Paskuliakova, A., Tonry, S., and Touzet, N., 2016, Phycoremediation of landfill leachate with chlorophytes: Phosphate a limiting factor on ammonia nitrogen removal, Water Res., 99, 180–187.

[25] Khanzada, Z.T., and Övez, S., 2017, Microalgae as a sustainable biological system for improving leachate quality, Energy, 140, 757–765.

[26] Beltrán-Rocha, J.C., Guajardo-Barbosa, C., Barceló-Quintal, I., and López-Chuken, U.J., 2017, Biotratamiento de efluentes secundarios municipales utilizando microalgas: Efecto del pH, nutrientes (C, N y P) y enriquecimiento con CO₂, Rev. Biol. Mar. Oceanogr., 52 (3), 417–427.

[27] Cai, T., Park, S.Y., and Li, Y., 2013, Nutrient recovery from wastewater streams by microalgae: Status and prospects, Renewable Sustainable Energy Rev., 19, 360–369.

[28] de Souza, L., Lima, A.S., Matos, Â.P., Wheeler, R.M., Bork, J.A., Vieira Cubas, A.L., and Moecke, E.H.S., 2021, Biopolishing sanitary landfill leachate via cultivation of lipid-rich Scenedesmus microalgae, J. Cleaner Prod., 303, 127094.

[29] Umamaheswari, J., and Shanthakumar, S., 2016, Efficacy of microalgae for industrial wastewater treatment: A review on operating conditions, treatment efficiency and biomass productivity, Rev. Environ. Sci. Bio/Technol., 15 (2), 265–284.

[30] Hernández-García, A., Velásquez-Orta, S.B., Novelo, E., Yáñez-Noguez, I., Monje-Ramírez, I., and Orta Ledesma, M.T., 2019, Wastewater-leachate treatment by microalgae: Biomass, carbohydrate and lipid production, Ecotoxicol. Environ. Saf., 174, 435–444.

[31] Zhao, X., Zhou, Y., Huang, S., Qiu, D., Schideman, L., Chai, X., and Zhao, Y., 2014, Characterization of microalgae-bacteria consortium cultured in landfill leachate for carbon fixation and lipid production, Bioresour. Technol., 156, 322–328.

DOI: https://doi.org/10.22146/ijc.82483