Antioxidant Activity of Fungus Comb Extracts Isolated from Indo-Malayan Termite  Macrotermes gilvus  Hagen (Isoptera: Termitidae)

Yanti Rachmayanti; Dikhi Firmansyah; Reza Ro'isatul Umma; Decsa Medika Hertanto; I Ketut Sudiana; Djoko Santoso; Dodi Nandika; Lina Karlinasari; Arinana Arinana; Irmanida Batubara; Lucia Dhiantika Witasari

doi:10.22146/ijc.77227

Antioxidant Activity of Fungus Comb Extracts Isolated from Indo-Malayan Termite Macrotermes gilvus Hagen (Isoptera: Termitidae)

https://doi.org/10.22146/ijc.77227

Yanti Rachmayanti^(1*), Dikhi Firmansyah⁽²⁾, Reza Ro'isatul Umma⁽³⁾, Decsa Medika Hertanto⁽⁴⁾, I Ketut Sudiana⁽⁵⁾, Djoko Santoso⁽⁶⁾, Dodi Nandika⁽⁷⁾, Lina Karlinasari⁽⁸⁾, Arinana Arinana⁽⁹⁾, Irmanida Batubara⁽¹⁰⁾, Lucia Dhiantika Witasari⁽¹¹⁾

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung 40132, West Java, Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung 40132, West Java, Indonesia
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Institut Teknologi Bandung, Bandung 40132, West Java, Indonesia
(4) Department of Internal Medicine, Faculty of Medicine, Universitas Airlangga, Campus A, Jl. Mayjen Prof. Dr. Moestopo 47 Surabaya, 60132, East Java, Indonesia
(5) Department of Pathology, Faculty of Medicine, Universitas Airlangga, Campus A, Jl. Mayjen Prof. Dr. Moestopo 47 Surabaya, 60132, East Java, Indonesia
(6) Department of Pathology, Faculty of Medicine, Universitas Airlangga, Campus A, Jl. Mayjen Prof. Dr. Moestopo 47 Surabaya, 60132, East Java, Indonesia
(7) Department of Forest Products, Faculty of Forestry and Environment, IPB University, Darmaga Campus, Bogor 16680, West Java, Indonesia
(8) Department of Forest Products, Faculty of Forestry and Environment, IPB University, Darmaga Campus, Bogor 16680, West Java, Indonesia
(9) Department of Forest Products, Faculty of Forestry and Environment, IPB University, Darmaga Campus, Bogor 16680, West Java, Indonesia
(10) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Tropical Biopharmaca Research Center, IPB University, Darmaga Campus, Bogor 16680, West Java, Indonesia
(11) Department of Food and Agricultural Product Technology, Faculty of Agricultural Technology, Universitas Gadjah Mada, Bulaksumur, Yogyakarta, 55281, Indonesia
(*) Corresponding Author

Abstract

Indonesian termites are one of the wealthiest biological resources considering their very high species diversity (300 species) and extensive geographical distribution in the Indonesian mainland. However, its potential as a source of bioactive compounds has not been fully explored. One of the endemic termite sub-families of Indonesian, Macrotermitinae (Isoptera: Termitidae), has a food source in the form of fungal nodules that grow and spread in a particular structure in the termite nest that is shaped like a mammalian brain called a fungus comb, which likely serves as a source of bioactive compounds. This study aims to analyze the potential of fungus comb from an Indonesian Macrotermitinae sub-family species as a source of antioxidants. Antioxidant activity was tested on four different extracts of fungus comb (EFC), namely hexane extract, ethyl acetate extract, methanol extract and water extract, using DPPH• (1,1-diphenyl-2-picrylhydrazyl) and ABTS•+ (2,2′-azino-di-[3-ethylbenzthiazolinesulphonate]) radicals. The results showed that the highest antioxidant activity was found in methanol extract, followed by ethyl acetate extract with IC₅₀ values of 0.6 mg/mL and 0.75 mg/mL, respectively. TEAC (Trolox equivalent antioxidant capacity) analysis confirmed the high antioxidant activity of methanol extract.

Keywords

Macrotermitinae; fungus comb extract; antioxidant; ABTS•+; DPPH•; IC50; TEAC

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DOI: https://doi.org/10.22146/ijc.77227