Steroids from  Atactodea striata  and Their Cytotoxic Activity against MCF-7 Breast Cancer Cell Lines

Evan Hadrian; Aprilia Permata Sari; Tri Mayanti; Desi Harneti; Rani Maharani; Darwati Darwati; Kindi Farabi; Unang Supratman; Celcius Waranmaselembun; Supriatno Salam; Sofa Fajriah; Muhamad Nurul Azmi

doi:10.22146/ijc.76438

Steroids from Atactodea striata and Their Cytotoxic Activity against MCF-7 Breast Cancer Cell Lines

https://doi.org/10.22146/ijc.76438

Evan Hadrian⁽¹⁾, Aprilia Permata Sari⁽²⁾, Tri Mayanti⁽³⁾, Desi Harneti⁽⁴⁾, Rani Maharani⁽⁵⁾, Darwati Darwati⁽⁶⁾, Kindi Farabi⁽⁷⁾, Unang Supratman^(8*), Celcius Waranmaselembun⁽⁹⁾, Supriatno Salam⁽¹⁰⁾, Sofa Fajriah⁽¹¹⁾, Muhamad Nurul Azmi⁽¹²⁾

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(4) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(5) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(6) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(7) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(8) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia; Central Laboratory, Universitas Padjadjaran, Jl. Raya Bandung-Sumedang Km. 21, Jatinangor, Sumedang 45363, Indonesia
(9) Department of Fisheries Biotechnology, State Fisheries Polytechnic of Tual, Tual 97613, Southeast Maluku, Indonesia
(10) Faculty of Pharmacy, Universitas Mulawarman, Samarinda, 75123, East Kalimantan, Indonesia
(11) Research Center for Raw Materials for Medicine and Traditional Medicine, National Research and Innovation Agency (BRIN), Tangerang Selatan, Banten 15314, Indonesia
(12) School of Chemical Sciences, Universiti Sains Malaysia, 11800 Minden, Penang, Malaysia
(*) Corresponding Author

Abstract

Marine environment is known as a source of potential steroids with multiple biological activities, one of which is an anticancer agent. Atactodea striata are one of the seashells distributed in Indonesia located in the Kei Islands, Southeast Maluku. During the course of our continuing search for biologically active substances from Indonesia seashells, seven steroids have been isolated from the n-hexane fraction of A. striata and they were identified as 7β-hydroxy-sitosterol (1), campesterol (2), β-sitosterol (3), cholesterol (4), 5α,8α-epidioxycholest-6-en-3-β-ol (5), 7-keto-cholesterol (6), and 7α-hydroxy-cholesterol (7). The structure was identified by spectroscopic methods including 2D NMR techniques, FTIR, HRTOFMS, and chemical shift comparison with previously reported spectral data. Compounds 1-7 were evaluated for their cytotoxic effects against MCF-7 breast cancer cells and showed weak or no anticancer activity.

Keywords

Atactodea striata; cytotoxic activity; MCF-7; steroids

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DOI: https://doi.org/10.22146/ijc.76438