Microstructural Analysis and Antibacterial Response of Zn2+/Mg2+ Dual Doped β-Tricalcium Phosphate Bioceramics

https://doi.org/10.22146/ijc.72286

Ammar Zeidan Alshemary(1*), Huda Basim Qasim(2), Ali Taha Saleh(3)

(1) Department of Biomedical Engineering, Faculty of Engineering, Karabuk University, Karabuk 78050, Turkey Biomedical Engineering Department, Al-Mustaqbal University College, Hillah, Babil 51001, Iraq
(2) Al-Manara College for Medical Sciences, Misan 62001, Iraq
(3) Department of Chemistry, College of Science, University of Misan, Misan 62001, Iraq
(*) Corresponding Author

Abstract


This article evaluates the impact of the addition of zinc (Zn) and magnesium (Mg) on the structural, morphological, and antibacterial characteristics of β-tricalcium phosphates (hereafter called Zn/Mg-βTCP) prepared using the microwave (MW) assisted wet precipitation method in which the Ca deficient apatite [Ca9-(x+y)MgxZny(HPO4)(PO4)5 (OH)] was calcined for 2 h at 1000 °C. The prepared samples were characterized using XRD, FTIR, and FESEM measurements. The XRD patterns of the samples showed a steady decrease in the lattice parameters with an increase in Mg2+ and Zn2+ content. The FESEM images of the samples disclosed the morphological changes due to the Mg2+/Zn2+ co-doping. The inclusion of Mg2+ and Zn2+ into the βTCP was shown to induce excellent bioactivities that were absent in the pristine βTCP. Enhancement, coupled with good antimicrobial properties against Escherichia coli (E. coli), suggests that Mg2+/Zn2+ co-doping TCP can be developed further into antibacterial bone cement. As synthesized, it would be considered a potential biomaterial for orthopedic applications.


Keywords


β-tricalcium phosphates; Co-doping; microstructure; phase purity; antibacterial

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References

[1] Kumar, G.S., Govindan, R., and Girija, E., 2014, In situ synthesis, characterization and in vitro studies of ciprofloxacin loaded hydroxyapatite nanoparticles for the treatment of osteomyelitis, J. Mater. Chem. B, 2 (31), 5052–5060.

[2] Shefy-Peleg, A., Foox, M., Cohen, B., and Zilberman, M., 2014, Novel antibiotic-eluting gelatin-alginate soft tissue adhesives for various wound closing applications, Int. J. Polym. Mater. Polym. Biomater., 63 (14), 699–707.

[3] Mouriño, V., and Boccaccini, A.R., 2010, Bone tissue engineering therapeutics: controlled drug delivery in three-dimensional scaffolds, J. R. Soc. Interface, 7 (43), 209–227.

[4] Feng, W., Geng, Z., Li, Z., Cui, Z., Zhu, S., Liang, Y., Liu, Y., Wang, R., and and Yang, X., 2016, Controlled release behaviour and antibacterial effects of antibiotic-loaded titania nanotubes, Mater. Sci. Eng., C, 62, 105–112.

[5] Dai, J., Fu, Y., Chen, D., and Sun, Z., 2021, A novel and injectable strontium-containing hydroxyapatite bone cement for bone substitution: A systematic evaluation, Mater. Sci. Eng., C, 124, 112052.

[6] Han, B., Ma, P.W., Zhang, L.L., Yin, Y.J., Yao, K.D., Zhang, F.J., Zhang, Y.D., Li, X.L., and Nie, W., 2009, β-TCP/MCPM-based premixed calcium phosphate cements, Acta Biomater., 5 (8), 3165–3177.

[7] Wang, S., Liu, R., Yao, J., Wang, Y., Li, H., Dao, R., Guan, J., and Tang, G., 2013, Fabrication of mesoporous magnesium substituted β-tricalcium phosphate nanospheres by self-transformation and assembly involving EDTA ions, Microporous Mesoporous Mater., 179, 172–181.

[8] Prakash, C., Singh, S., Pabla, B.S., Sidhu, S.S., and Uddin, M.S., 2019, Bio-inspired low elastic biodegradable Mg-Zn-Mn-Si-HA alloy fabricated by spark plasma sintering, Mater. Manuf. Processes, 34 (4), 357–368.

[9] Horiuchi, S., Hiasa, M., Yasue, A., Sekine, K., Hamada, K., Asaoka, K., and Tanaka, E., 2014, Fabrications of zinc-releasing biocement combining zinc calcium phosphate to calcium phosphate cement, J. Mech. Behav. Biomed. Mater., 29, 151–160.

[10] Kaygili, O., Keser, S., Bulut, N., and Ates, T., 2018, Characterization of Mg-containing hydroxyapatites synthesized by combustion method, Physica B, 537, 63–67.

[11] Calasans-Maia, M., Calasans-Maia, J., Santos, S., Mavropoulos, E., Farina, M., Lima, I., Lopes, R.T., Rossi, A., and Granjeiro, J.M., 2014, Short-term in vivo evaluation of zinc-containing calcium phosphate using a normalized procedure, Mater. Sci. Eng., C, 41, 309–319.

[12] Shahmohammadi, P., and Khazaei, B.A., 2021, Characterization of Zn/Mg-enriched calcium phosphate coating produced by the two-step pulsed electrodeposition method on titanium substrate, Surf. Interfaces, 22, 100819.

[13] Saleh, A.T., Ling, L.S., and Hussain, R., 2016, Injectable magnesium-doped brushite cement for controlled drug release application, J. Mater. Sci., 51 (16), 7427–7439.

[14] Tas, A.C., Bhaduri, S.B., and Jalota, S., 2007, Preparation of Zn-doped β-tricalcium phosphate (β-Ca3(PO4)2) bioceramics, Mater. Sci. Eng., C, 27 (3), 394–401.

[15] Mahdavi-Roshan, M., Ebrahimi, M., and Ebrahimi, A., 2015, Copper, magnesium, zinc and calcium status in osteopenic and osteoporotic post-menopausal women, Clin. Cases Miner. Bone Metab., 12 (1), 18–21.

[16] Fadeeva, I.V., Gafurov, M.R., Kiiaeva, I.A., Orlinskii, S.B., Kuznetsova, L.M., Filippov, Y.Y., Fomin, A.S., Davydova, G.A., Selezneva, I.I., and Barinov, S.M., 2017, Tricalcium phosphate ceramics doped with silver, copper, zinc, and iron (III) ions in concentrations of less than 0.5 wt.% for bone tissue regeneration, BioNanoScience, 7 (2), 434–438.

[17] Chen, X., Tang, Q.L., Zhu, Y.J., Zhu, C.L., and Feng, X.P., 2012, Synthesis and antibacterial property of zinc loaded hydroxyapatite nanorods, Mater. Lett., 89, 233–235.

[18] Bhattacharjee, A., Gupta, A., Verma, M., Murugan, P.A., Sengupta, P., Matheshwaran, S., Manna, I., and Balani, K., 2019, Site-specific antibacterial efficacy and cyto/hemo-compatibility of zinc substituted hydroxyapatite, Ceram. Int., 45 (9), 12225–12233.

[19] Hofmann, M.P., Mohammed, A.R., Perrie, Y., Gbureck, U., and Barralet, J.E., 2009, High-strength resorbable brushite bone cement with controlled drug-releasing capabilities, Acta Biomater., 5 (1), 43–49.

[20] Frasnelli, M., Pedranz, A., Biesuz, M., Dirè, S., and Sglavo, V.M., 2019, Flash sintering of Mg-doped tricalcium phosphate (TCP) nanopowders, J. Eur. Ceram. Soc., 39 (13), 3883–3892.

[21] Saleh, A.T., and Alameri, D., 2021, Microwave-assisted preparation of zinc-doped β-tricalcium phosphate for orthopedic applications, Indones. J. Chem, 21 (2), 376–382.

[22] Motameni, A., Dalgic, A.D., Alshemary, A.Z., Keskin, D., and Evis, Z., 2020, Structural and biological analysis of mesoporous lanthanum doped βTCP for potential use as bone graft material, Mater. Today Commun., 23, 101151.

[23] Qin, L., Yi, J., Xuefei, L., Li, L., Kenan, X., and Lu, X., 2020, The preparation of a difunctional porous β-tricalcium phosphate scaffold with excellent compressive strength and antibacterial properties, RSC Adv., 10, 28397–28407.



DOI: https://doi.org/10.22146/ijc.72286

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