In Vitro Alpha-Amylase Inhibitory Activity of Microencapsulated  Cosmos caudatus  Kunth Extracts

Anna Safitri; Anna Roosdiana; Ellysia Hitdatania; Savira Ayu Damayanti

doi:10.22146/ijc.68844

In Vitro Alpha-Amylase Inhibitory Activity of Microencapsulated Cosmos caudatus Kunth Extracts

https://doi.org/10.22146/ijc.68844

Anna Safitri^(1*), Anna Roosdiana⁽²⁾, Ellysia Hitdatania⁽³⁾, Savira Ayu Damayanti⁽⁴⁾

(1) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran, Malang, 65145, Indonesia Research Centre of SMONAGENES (Smart Molecules of Natural Genetic Resources), Brawijaya University, Indonesia
(2) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran, Malang, 65145, Indonesia
(3) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran, Malang, 65145, Indonesia
(4) Department of Chemistry, Faculty of Mathematics and Natural Sciences, Brawijaya University, Jl. Veteran, Malang, 65145, Indonesia
(*) Corresponding Author

Abstract

The existence of phytochemicals in Cosmos caudatus Kunth, predominantly phenolic compounds, offers several health benefits. Nevertheless, the bioactive compounds are usually susceptible to degradation, and therefore may reduce their biological activity. This work aims to carry out microencapsulation of C. caudatus K. extracts by spray drying technique. The in vitro alpha-amylase inhibitory activity of the microencapsulated product is also investigated. The effect of manufacturing conditions, including pH, the concentration of wall materials, and stirring time, was evaluated. The optimal conditions for microcapsules formation were selected based on the activity of microcapsules as inhibitors for the alpha-amylase enzyme, pointing out by the lowest number of IC₅₀. Results showed that microcapsules prepared in pH 4, 0.05% of chitosan, and 90 min stirring time had optimum efficiency, with the IC₅₀ value of 92.85 ± 1.21 μg/mL. The FTIR (Fourier-Transform infrared) analysis showed that the –C–N stretching amine functional group appeared at wavenumber 1285 cm^–1, and the –P=O phosphate bending appeared at 1206 cm^–1. Characterization with PSA (particle size analyzer) and SEM (scanning electron microscope) indicated that microcapsules had predominantly spherical forms with a mean diameter of 38.92 μm. This work confirms the important role of microencapsulation in developing plant extracts with retained biological functionalities.

Keywords

chitosan; Cosmos caudatus Kunth; microencapsulation; spray-drying

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References

[1] Zimmet, P.Z., 2017, Diabetes and its drivers: the largest epidemic in human history?, Clin. Diabetes Endocrinol., 3 (1), 1.

[2] Zimmet, P.Z., Alberti, K.G., Magliano, D.J., and Bennet, P.H., 2016, Diabetes mellitus statistics on prevalence and mortality: Facts and fallacies, Nat. Rev. Endocrinol., 12 (10), 616–622.

[3] Kerner, W. and Brückel, J., 2014, Definition, classification and diagnosis of diabetes mellitus, Exp. Clin. Endocrinol. Diabetes, 122 (7), 384–386.

[4] Filippatos, T.D., Panagiotopoulou, T.V., and Elisaf, M.S., 2014, Adverse effects of GLP-1 receptor agonists, Rev. Diabet. Stud., 11 (3-4), 202–230.

[5] Nesti, L. and Natali, A., 2017, Metformin effects on the heart and the cardiovascular system: A review of experimental and clinical data, Nutr., Metab. Cardiovasc. Dis., 27 (8), 657–669.

[6] Wang, Y.W., He, S.J., Feng, X., Cheng, J., Luo, Y.T., Tian, L., Huang, Q., 2017, Metformin: a review of its potential indications, Drug Des., Dev. Ther., 11, 2421–2429.

[7] Safitri, A., Srihardyastutie, A., Roosdiana, A., Aulanni'am, A., and Octaviana, E.N.L., 2019, Effects of root extract of Ruellia tuberosa L. on kidneys of diabetic rats, J. Math. Fundam. Sci., 51 (2), 127–137.

[8] Roosdiana, A., Permata, F.S., Fitriani, R.I., Umam, K., and Safitri, A., 2020, Ruellia tuberosa L. extract improves histopathology and lowers malondialdehyde levels and TNF alpha expression in the kidney of streptozotocin-induced diabetic rats, Vet. Med. Int., 2020, 8812758.

[9] Siahaan, P., Mentari, N.C., Wiedyanto, U.O., Hudiyanti, D., Hildayani, S.Z., and Laksitorini, M.D., 2017, The optimum conditions of carboxymethyl chitosan synthesis on drug delivery application and its release of kinetics study, Indones. J. Chem., 17 (2), 291–300.

[10] Bunawan, H., Baharum, S.N., Bunawan, S.N., Mat Amin, N., and Noor, N.M., 2014, Cosmos caudatus Kunth: A traditional medicinal herb, Global J. Pharmacol., 8 (3), 420–426.

[11] Abdullah, A., Dhaliwal, K.K., Roslan, N.N.F., Lee, C.H., Kalaiselvam, M., Radman, H.M., Haji Mohd Saad, Q., Yusof, K., and Jaarin, K., 2015, The effects of Cosmos caudatus (ulam raja) on detoxifying enzymes in extrahepatic organs in mice, J. Appl. Pharm. Sci., 5 (1), 82–88.

[12] Safitri, A., Putri, A.S., Octavianty, T.D., and Sari, D.R.T., 2020, Metabolomic profiles of Curcuma longa L and Cosmos caudatus extracts and their in-silico anti-cancer activity, J. Phys.: Conf. Ser., 1665, 012022.

[13] Chen, L., Gnanaraj, C., Arulselvan, P., El-Seedi, H., and Teng, H., 2019, A review on advanced microencapsulation technology to enhance bioavailability of phenolic compounds: Based on its activity in the treatment of type 2 diabetes, Trends Food Sci. Technol., 85, 149–162.

[14] Suratman, A., Purwaningsih, D.R., Kunarti, E.S., and Kuncaka, A., 2020, Controlled release fertilizer encapsulated by glutaraldehyde-crosslinked chitosan using freeze-drying method, Indones. J. Chem., 20 (6), 1414–1421.

[15] Ćujić-Nikolić, N., Stanisavljević, N., Šavikin, K., Kalušević, A., Nedović, V., Samardžić, J., and Janković, T., 2019, Chokeberry polyphenols preservation using spray drying: Effect of encapsulation using maltodextrin and skimmed milk on their recovery following in vitro digestion, J. Microencapsulation, 36 (8), 693–703.

[16] Lucas, J., Ralaivao, M., Estevinho, B.N., and Rocha, F., 2020, A new approach for the microencapsulation of curcumin by a spray drying method, in order to value food products, Powder Technol., 362, 428–435.

[17] Lestari, A.D.N, Siswanta, D., Martien, R., and Mudasir, M., 2020, Synthesis, characterization, and stability evaluation of β-carotene encapsulated in starch-chitosan/tripolyphosphate matrices, Indones. J. Chem., 20 (4), 929–940.

[18] Abdelkader, H., Hussain, S.A., Abdullah, N., and Kmaruddin, S., 2018, Review on micro-encapsulation with chitosan for pharmaceuticals applications, MOJ Curr. Res. Rev., 1 (2), 77–84.

[19] Jayanudin, J, Fahrurrozi, M., Wirawan, S.K., and Rochmadi, R., 2019, Preparation of chitosan microcapsules containing red ginger oleoresin using emulsion crosslinking method, J. Appl. Biomater. Funct. Mater., 17 (1), 1-9.

[20] Sacco, P., Paoletti, S., Cok, M., Asaro, F., Abrami, M., Grassi, M., and Donati, I., 2016, Insight into the ionotropic gelation of chitosan using tripolyphosphate and pyrophosphate as cross-linkers, Int. J. Biol. Macromol., 92, 476–483.

[21] Sacco, P., Pedroso-Santana, S., Kumar, Y., Joly, N., Martin, P., and Bocchetta, P., 2021, Ionotropic gelation of chitosan flat structures and potential applications, Molecules, 26 (3), 660.

[22] Goh, C.Y., Lim, S.S., Tshai, K.Y., El Azab, A.W.Z.Z., and Loh, H. S., 2019, Fabrication and in vitro biocompatibility of sodium tripolyphosphate-crosslinked chitosan–hydroxyapatite scaffolds for bone regeneration, J. Mater. Sci., 54 (4), 3403–3420.

[23] Ang, L.F., Darwis, Y., Por, L.Y., and Yam, M.F., 2019, Microencapsulation curcuminoids for effective delivery in pharmaceutical application, Pharmaceutics, 11 (9), 451.

[24] Singh, M.N., Hemant, K.S.Y., Ram, M., and Shivakumar, H.G., 2010, Microencapsulation: A promising technique for controlled drug delivery, Res. Pharm. Sci., 5 (2), 65–77.

[25] Özkan, G. and Bilek, S.E., 2014, Microencapsulation of natural food colourants, Int. J. Nutr. Food Sci., 3 (3), 145–156.

[26] Kashif, P.M., Madni, A., Ashfaq, M., Rehman, M., Mahmood, M.A., Khan, M.I., and Tahir, N., 2017, Development of Eudragit RS 100 microparticles loaded with ropinirole: Optimization and in vitro evaluation studies, AAPS PharmSciTech, 18 (5), 1810–1822.

[27] Oyedemi, S.O., Oyedemi, B.O., Ijeh, I.I., Ohanyerem, P.E., Coopoosamy, R.M., and Aiyegoro, O.A., 2017, Alpha-amylase inhibition and antioxidative capacity of some anti-diabetic plants used by the traditional healers in Southeastern Nigeria, Sci. World J., 2017, 3592491.

[28] Safitri, A., Fatchiyah, F., Sari, D.R.T., and Roosdiana, 2020, Phytochemical screening, in vitro anti-oxidant activity, and in silico anti-diabetic activity of aqueous extracts of Ruellia tuberosa L, J. Appl. Pharm. Sci., 10 (3), 101–108.

[29] Prasad, B.J., Sharavanan, P.S., and Sivaraj, R., 2019, Efficiency of Oryza punctata extract on glucose regulation: Inhibition of α-amylase and α-glucosidase activities, Grain Oil Sci. Technol., 2 (2), 44–48.

[30] DiNicolantonio, J.J., Bhutani, J., and O'Keefe, J.H., 2015, Acarbose: Safe and effective for lowering postprandial hyperglycaemia and improving cardiovascular outcomes, Open Heart, 2 (1), e000327.

[31] Oyedemi, S., Koekemoer, T., Bradley, G., Van De Venter, M., and Afolayan, A., 2013, In vitro anti-hyperglycemia properties of the aqueous stem bark extract from Strychnos henningsii (Gilg), Int. J. Diabetes Dev. Countries, 33 (2), 120–127.

[32] Tan, C., Xie, J., Zhang, X., Cai, J., and Xia, S., 2016, Polysaccharide-based nanoparticles by chitosan and gum Arabic polyelectrolyte complexation as carriers for curcumin, Food Hydrocolloids, 57, 236–245.

[33] Sharpe, L.A., Vela Ramirez, J.E., Haddadin, O.M., Ross, K.A., Narasimhan, B., and Peppas, N.A., 2018, pH-Responsive microencapsulation systems for the oral delivery of polyanhydride nanoparticles, Biomacromolecules, 19 (3), 793–802.

[34] Suzery, M., Hadiyanto, Majid, D., Setyawan, D., and Sutanto, H., 2017, Improvement of stability and antioxidant activities by using phycocyanin-chitosan encapsulation technique, IOP Conf. Ser.: Earth Environ. Sci., 55, 012052.

[35] Souza, J.M., Caldas, A.L., Tohidi, S.D., Molina, J., Souto, A.P., Fangueiro, R., Zille, A., 2014, Properties and controlled release of chitosan microencapsulated limonene oil, Rev. Bras. Farmacogn., 24 (6), 691–698.

[36] Gierszewska-Drużyńska, M., and Ostrowska-Czubenko, J., 2010, The effect of ionic crosslinking on thermal properties of hydrogel chitosan membrane, Prog. Chem. Appl. Chitin Its Deriv., 15, 25–32.

[37] Azevedo, J.R., Sizilio, R.H., Brito, M.B., Costa, A.M.B., Serafini, M.R., Araujo, A.A.S., Santos, M.R.V., Lira, A.A.M., and Nunes, R.S., 2011, Physical and chemical characterization insulin-loaded chitosan-TPP nanoparticles, J. Therm. Anal. Calorim., 106, 685–689.

[38] Kartini K., Putri, L.A.D., and Hadiyat, M.A., 2020, FTIR-based fingerprinting and discriminant analysis of Apium graveolens from different locations, J. Appl. Pharm. Sci., 10 (12), 62–67.

[39] Patle, T.K., Shrivas, K., Kurrey, R., Upadhyay, S., Jangde, R., and Chauhan, R., 2020. Phytochemical screening and determination of phenolics and flavonoids in Dillenia pentagyna using UV–vis and FTIR spectroscopy, Spectrochim. Acta, Part A, 242, 118717.

DOI: https://doi.org/10.22146/ijc.68844