IMMOBILIZATION OF GLUCOSE OXIDASE ENZYME FROM Penicillium sp-3 LOCAL STRAIN

https://doi.org/10.22146/ijc.21721

Ahyar Ahmad(1*), Andi Syaiful(2), Firman AP Firman AP(3), Abdul Rauf Patong(4)

(1) Biochemistry and Biotechnology Laboratory, Department of Chemistry, Faculty of Natural Sciences, Hasanuddin University, Makassar, 90245
(2) Biochemistry and Biotechnology Laboratory, Department of Chemistry, Faculty of Natural Sciences, Hasanuddin University, Makassar, 90245
(3) Bioprocess Laboratory, Ujung Pandang State Polytechnic, Makassar, 90245.
(4) Biochemistry and Biotechnology Laboratory, Department of Chemistry, Faculty of Natural Sciences, Hasanuddin University, Makassar, 90245
(*) Corresponding Author

Abstract


Immobilization of glucose oxidase (GOD) enzyme from Penicillium sp-3 local strain has been carried out using ionotropically entrapping method in  Ca-alginate membrane coupled with Na-polyacrilate. The entrapment of the enzyme in diffusion membrane occur spontaneously by cross-linking between Na-alginate/Na-polyacrilate and CaCl2. The GOD enzyme immobilizedby addition of 1 % Na-polyacrilate has the highest encapsulation efficiency, that is 87.13 % with the smallest percentage of diffusion, i.e. 23.37% and the relative activity of 50%. The GOD immobilized enzyme had good stability at the pH range 4 - 7 during 30 minutes of storage and was stable at a temperature of 20 oC. The activity of the GOD enzyme after being utilized continuously for 5 times only decrease up to 47,06 % compared to that in the initial utilization.


Keywords


immobilization; glucose oxidase; Penicillium sp-3; calcium alginate; sodium polyacrilate

Full Text:

Full Text PDF


References

[1] Swaisgood, H.E., 1985, Immobilization of Enzymes and Some Aplications in the Food Industry., in A.I. Laskin (Ed.), Enzymes and Immobilized Cells in Biotechnology-(Biotechnology Series) 1-24, The Benjamin/Cummings Publishing Company, Inc., California.

[2] Sudarmadji, S., Haryono, B. and Suhardi, 1984, Prosedur Analisa Untuk Bahan Makanan dan Pertanian (Edisi ketiga)., Liberty, Yogyakarta, 51-57.

[3] Aubrée-Lecat, A., C. Hervagault, A. Delacour, P. Beaude, C. Bourdillon and Remy, M.1989, Anal. Biochem., 178 (2), 427-430.

[4] Miao, Y., Chia, L. S. Goh, N. K. and Tan, S. N. 2000, Analyst, 125, 1591-1594.

[5] Brooks, S. L., Ashby, R. E. Turner, A. P. F. Calder M. R. and Clarke, D. J. 1987, Biosensors, 4 (1), 68-71

[6] Firman, A.P. and Aryantha, I.N.P., 2003, Eksplorasi dan Isolasi Enzim Glukosa Oksidase dari Fungi Genus Aspergillus dan Penicillium Galur Indogenus, Laporan Hasil Penelitian Dasar Lembaga Penelitian ITB, Bandung.

[7] Harlander, S. K, 1989, Biotechnology Opportunities for the Food Industry., in Rogers, P. L. and Fleet, G. H. (Ed.), Biotechnology and the Food Industry (1-15), Gordon And Breach Science Publishers, Sydney-Australia.

[8] Chibata, I., Tosa, T. and Sato, T. 1985, Immobilized Biocatalysts to Produce Amino Acids and Other Organic Compounds., in Laskin A. I. (Ed.), Enzymes and Immobilized Cells in Biotechnology: Biotechnology Series (37-70), The Benjamin/Cummings Publishing Company, Inc., California.

[9] Brodelius, P., 1985, Immobilized Plant Cells., in Laskin A. I. (Ed.), Enzymes and Immobilized Cells in Biotechnology: Biotechnology Series (109-148), The Benjamin/Cummings Publishing Company, Inc., California.

[10] Lyubov, K. Y. dan Ivanov, I. P., 1996, Appl. Biochem. Biotech., 61, 277-287.

[11] Suye, S-I, Y. Kumon, A. and Ishigaki, I., 1998, Biotechnol. Appl. Biochem., 27, 245-248

[12] Onda, M., Ariga, K. and Kunitake, T., 1999, J. Biosci. Bioeng., 87 (1) 69-75.

[13] Trau, D. and Renneberg, R., 2003, Biosens. Bioelec., 18, 1491-1499.

[14] Russel, L. J. and Rawson, K. M., 1986, Biosensors, 2 (1), 25-31

[15] Weetall, H. H. and Hotaling, T., 1987, Biosensors, 3 (2), 57-63

[16] Blandino, A., M. Macias, D. and Cantero, 2000, Enzyme. Microb. Tech., 27, 319-324.

[17] Lowry, O. H., Rosebrough, N. J. Farr A. L. and Randall, R. J. 1951, J. Biol. Chem., 193, 265-275.

[18] Worthington, C. C., 1988, Worthington Enzyme Manual: Enzyme and Related Biochemical., Worthington Biobhemical Co., USA (155-158).

[19] Worthington Glucose Oxidase, Worthington Glucose Oxidase: Manual Page., (Online): http://www.worthington_biochem.com/GOP/default.html

[20] Hatzinikolaou, D. G. and Macris, B. J. 1995, Enzyme. Microb. Technol., 17, 530-534.



DOI: https://doi.org/10.22146/ijc.21721

Article Metrics

Abstract views : 2772 | views : 3247


Copyright (c) 2010 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Web Analytics View The Statistics of Indones. J. Chem.