PRODUCTION AND CHARACTERIZATION OF BIOSURFACTANT BY Pseudomonas fluorescens USING CASSAVA FLOUR WASTEWATER AS MEDIA

https://doi.org/10.22146/ijc.21281

Venty Suryanti(1*), Soerya Dewi Marliyana(2), Desi Suci Handayani(3), Desi Ratnaningrum(4)

(1) Department of Chemistry, Faculty of Mathematic and Natural Sciences, Sebelas Maret University Jl. Ir. Sutami 36A, Surakarta, Central Java 57126
(2) Department of Chemistry, Faculty of Mathematic and Natural Sciences, Sebelas Maret University Jl. Ir. Sutami 36A, Surakarta, Central Java 57126
(3) Department of Chemistry, Faculty of Mathematic and Natural Sciences, Sebelas Maret University Jl. Ir. Sutami 36A, Surakarta, Central Java 57126
(4) Department of Chemistry, Faculty of Mathematic and Natural Sciences, Sebelas Maret University Jl. Ir. Sutami 36A, Surakarta, Central Java 57126
(*) Corresponding Author

Abstract


Biosurfactant with efficient emulsification properties could be produced by Pseudomonas flourescens using cassava flour wastewater (manipueira) as media. The ability of P. flourescens to produce biosurfactant could suggest potential use in industrial and environmental applications. Media containing a mixture of natural manipueira and nutrient broth with 48 h fermentation was the optimum condition for the biosurfactant production. Based on UV-Vis and FT-IR spectra, the biosurfactant was indicated as rhamnolipids containing hydroxyl, ester, carboxylic and aliphatic carbon chain functional groups. Biosurfactant exhibited critical micelle concentration (CMC) value of 715 mg/L and reduced the surface tension of the water from 80 mN/m to 59 mN/m. The biosurfactant was able to decrease the interfacial tension about 51-70% when benzyl chloride, palm oil and kerosene were used as water-immiscible compounds. The biosurfactant was able to form stable emulsion until 30 days when paraffin, soybean oil, lubricant oil and kerosene were used as water-immiscible compounds.

Keywords


biosurfactant; manipueira; Pseudomonas flourescens; rhamnolipids

Full Text:

Full Text PDF


References

[1] Zajic, J.E., Gignard, H., and Gerson, D.F., 1997, Biotechnol. Bioeng., 19, 1303–1320.

[2] Georgiou, G., Lin, S-C., and Sharma, M.M., 1992, Nat. Biotechnol., 10, 1, 60–65.

[3] Razafindralambo, H., Paquot, M., Baniel, A., Popineau, Y., Hbid, C., Jacques, P., and Thonart, P., 1996, J. Am. Oil Chem. Soc., 73, 149–151.

[4] Velikonja, J. and Kosaric, N., 1993, “Biosurfactant in food applications” in “Biosurfactants: Production, Properties, Applications”, Kosaric, N., Marcel Dekker Inc., New York, 419–446.

[5] Desai, J.D., and Banat, I.M., 1997, Microbiol. Mol. Biol. Rev., 61, 47–64.

[6] Suryanti, V., Hastuti, S., Wahyuningsih, T.W., Mudasir, and Muliawati, D.I., 2009, Indo. J. Chem., 9, 1, 107–112.

[7] Hernandes-Anguiano, A.M., Suslow, T.V., Leloup, L., and Kado C.I., 2004, Plant Panthol., 53, 5, 596–601.

[8] Nitschke, M., Ferraz, C., and Pastore, G.M., 2004, Braz. J. Microbiol., 35, 1-2, 81–85.

[9] Tahzibi, A., Kamal, F., and Assadi, M.M., 2004, Iran. Biomed. J., 8, 1, 25–31.

[10] Vasileva-Tonkova, E., Galabova, D., Stoimenova, E., and Lalchev, Z., 2006, Z. Naturforsch., C: Biosci., 61, 7-8, 553–559.

[11] Bodour, A.A., Drees, K.P., and Maier, R.M., 2003, Appl. Environ. Microbiol., 69, 6, 3280–3287.

[12] Bodour, A.A., Guerrero-Barajas, C., Jiorle, B.V., Malcomson, M.E., Paull, A.K., Somogyi, A., Trinh, L.N., Bates, R.B., and Maier, R.M., 2004, Appl. Environ. Microbiol., 70, 1, 114–120.

[13] Bodour, A.A., Drees, K.P., and Maier, R.M., 2003, Appl. Environ. Microbiol., 69, 3280–3287.

[14] Rashedi, H., Jamshidi, E., Assadi, M.M., and Bonakdarpour, B., 2005, Int. J. Environ. Sci. Technol., 2, 2, 121–127.

[15] Davey, M.E., Caiazza, N.C., and O’Toole, G.A., 2003, J. Bacteriol., 185, 3, 1027–1036.

[16] Lee, S-C., Yoo, J-S., Kim, S-H., Chung, S-Y., Hwang, C-W., Joo, W-H., and Choi, Y-L., 2006, J. Microbiol. Biotechnol., 16, 5, 716–723.

[17] Urum, K., and Pekdemir, T., 2004, Chemosphere, 57, 9, 1139–1150.

[18] Yoo, D-S., Lee, B-S., and Kim, E-K., 2005, J. Microbiol. Biotechnol., 15, 6, 1164–1169.

[19] Okpokwasili, G.C., and Ibiene, A.A., 2006, Afr. J. Biotechnol., 5, 5, 453–456.

[20] Mukherjee, S., Das, P., and Sen, R., 2006, Trends Biotechnol., 24, 11, 509–515.

[21] Henkel, M., Müller, M.M., Kügler, J.H., Lovaglio, R.B., Contiero, J., Syldatk, C., and Hausmann, R., 2012, Process Biochem., 47, 8, 1207–1219.

[22] Müller, M., and Hausmann, R., 2011, Appl. Microbiol. Biotechnol., 91, 2, 251–264.

[23] Abdel-Mawgoud, A., Lépine, F., and Déziel, E., 2010, Appl. Microbiol. Biotechnol., 86, 5, 1323–1336.

[24] Reis, R.S., da Rocha, S.L.G., Chapeaurouge, D.A., Domont, G.B., Santa Anna, L.M.M., Freire, D.M.G., and Perales, J., 2010, Process Biochem., 45, 9, 1504–1510.

[25] Reis, R.S., Pereira, A.G., Neves, B.C., and Freire, D.M.G., 2011, Bioresour. Technol., 102, 11, 6377–6384.

[26] Banat, I.M., Franzetti, A., Gandolfi, I., Bestetti, G., Martinotti, M.G., Fracchia, L., Smyth, T.J., and Marchant, R., 2010, Appl. Microbiol. Biotechnol., 87, 2, 427–444.

[27] Damasceno, F.R.C., Cammarota, M.C., and Freire, D.M.G., 2012, Colloids Surf., B, 95, 241–246.

[28] Shete, A.M., Wadhawa, G., Banat, I.M., and Chopade, B.A., 2006, J. Sci. Ind. Res., 65, 2, 91–115.

[29] Cameotra, S.S., and Makkar, R.S., 2010, Pure Appl. Chem., 82, 1, 97–116.

[30] Singh, S., Kang, S.H., Mulchandani, A., and Chen, W., 2008, Curr. Opin. Biotechnol., 19, 5, 437–444.

[31] Santa Anna, L.M.M., Soriano, A.U., Gomes, A.C., Menezes, E.P., Gutarra, M.L.E., Freire, D.M.G., and Pereira, Jr.N., 2007, J. Chem Technol. Biotechnol., 82, 1, 687–691.

[32] Whang, L-M., Liu, P-W.G., Ma, C-C., and Cheng, S-S., 2008, J. Hazard. Mater., 151, 1, 155–163.

[33] Zhou, W., and Zhu, L., 2007, Environ. Pollut., 147, 1, 66–73.

[34] Wei, Y-H., Chou, C-L., and Chang, J-S., 2005, Biochem. Eng. J., 27, 2, 146–154.

[35] Makkar, R.S., and Rockne, K.J., 2009, Environ. Toxicol. Chem., 22, 10, 2280–2292.

[36] Sheppard, J.D., and Mulligan, C., 1987, Appl. Microbiol. Biotechnol., 27, 2, 110–116.

[37] Mercadé, M.E., Manresa, M.A., Robert, M., Espuny, M.J., de Andrés, C., and Guinea, J., 1993, Bioresour. Technol., 43, 1, 1–6.

[38] Koch, A.K., Reiser, J., Käppeli, O., and Fiecter, A., 1988, Nat. Biotechnol., 6, 11, 1335–1339.

[39] Ohno, A., Takashi, A., and Shoda, M., 1995, Biotechnol. Bioeng., 47, 2, 209–214.

[40] Fox, S.L., and Bala, G.A., 2000, Bioresour. Technol., 75, 3, 235–240.

[41] Thompson, D.N., Fox, S.L., and Bala, G.A., 2000, Appl. Biochem. Biotechnol., 84-86, 917–929.

[42] Makkar, R.S., and Cameotra, S.S., 1997, J. Am. Oil Chem. Soc., 74, 7, 887–889.

[43] Patel, R.M., and Desai, A.J., 1997, J. Basic Microbiol., 37, 4, 281–286.

[44] Iqbal, S., Khalid, Z.M., and Malik, K.A., 1995, Lett. Appl. Microbiol., 21, 3, 176–179.

[45] Martin, A., Swarbrick, J., and Cammarata, A., 1983, Physical Pharmacy, Lea & Febiger Inc., Philadelphia, P.A., USA.

[46] Candrasekaran, E.V., and Bemiller, J.N., 1980, “Constituent analyses of glycosamino-glycans” in Methods in Carbohydrate Chemistry, Whistler, R.L., Academic Press Inc., New York, 89–96.

[47] Cirigliano, M.C., and Carman, G.M., 1985, Appl. Environ. Microbiol., 50, 4, 846–850.

[48] Sifour, M., Al-Jilawi, M.H., and Aziz, G.M., 2007, Pak. J. Biol. Sci., 10, 8, 1331–1335.

[49] Robert, M., Mercade, M.E., Bosch, M.P., Parra, J.L., Espuny, M.J., Manresa, M.A., and Guinea, J., 1989, Biotechnol. Lett., 11, 12, 871-874.

[50] Tuleva, B.J., Ivanov, G.R., and Christova, N.E., 2002, Z. Naturforsch., C: Biosci., 57, 3-4, 356–360.

[51] Bodour, A.A., and Miller-Maier, R.M., 1998, J. Microbiol. Methods, 32, 3, 273–280.

[52] Zhang, Y., and Miller, R.M., 1992, Appl. Environ. Microbiol., 58, 10, 3276–3282.

[53] Kim, J-S., Powalla, M., Lang, S., Wagner, F., Lünsdorf, H., and Wray, V., 1990, J. Biotechnol., 13, 4, 257–266.

[54] Rapp, P., Bock, H., Wray, V., and Wagner, F., 1979, J. Gen. Microbiol., 115, 2, 491–503.

[55] Kim, S.H., Lim, E.J., Lee, S.O., Lee, J.D., and Lee, T.H., 2000, Biotechnol. Appl. Biochem., 31, 3, 249–253.

[56] Lee, S-C., Jung, Y-J., Yoo, J-S., Cho, Y-S., Cha, I-H., and Choi, Y-L., 2002, Korean J. Life Sci., 12, 6, 745–751.

[57] Suk, W-S., Son, H-J., Lee, G., and Lee, S-J., 1999, J. Microbiol. Biotechnol., 9, 1, 56–61.



DOI: https://doi.org/10.22146/ijc.21281

Article Metrics

Abstract views : 2212 | views : 2499


Copyright (c) 2013 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Web Analytics View The Statistics of Indones. J. Chem.