In Vitro Antidiabetic Activities of Extract and Isolated Flavonoid Compounds from Artocarpus altilis (Parkinson) Fosberg

https://doi.org/10.22146/ijc.21261

Puspa Dewi Narrij Lotulung(1), Tjandrawati Mozef(2*), Chandra Risdian(3), Akhmad Darmawan(4)

(1) Research Center for Chemistry, Indonesian Institute of Sciences, Kampus LIPI Gd. 50, Jl. Sangkuriang Bandung
(2) Research Center for Chemistry, Indonesian Institute of Sciences, Kampus LIPI Gd. 50, Jl. Sangkuriang Bandung
(3) Research Center for Chemistry, Indonesian Institute of Sciences, Kampus LIPI Gd. 50, Jl. Sangkuriang Bandung
(4) Research Center for Chemistry, Indonesian Institute of Sciences, Kampus LIPI Gd. 50, Jl. Sangkuriang Bandung
(*) Corresponding Author

Abstract


The antidiabetic activity test through a mechanism of inhibition of α-glucosidase enzyme was studied against ethanol, n-hexane, ethyl acetate and n-butanol fractions of ethanol extract of Artocarpus altilis (Parkinson) Fosberg (Moraceae) leaves and four flavonoid compounds isolated from ethyl acetate extracts of A. altilis. Ethyl acetate fraction has strongest antidiabetic activity compared to ethanol, n-hexane, and n-butanol fractions with IC50values5.98,6.79, 440.18and14.42μg/mL, respectively. Four flavonoid compounds (1-(2,4-dihydroxyphenyl)-3-[8-hydroxy-2-methyl-2-(4-methyl-3-pentenyl)-2H-1-benzopyran-5-yl]-1-propanone (AC-31), 2-geranyl-2',3,4,4'-tetrahydroxy dihydrochalcone (AC-51), 8-geranyl-4',5,7-trihydroxyflavone (AC-33) andcyclocommunol (AA-3), have been isolated from ethylacetate fraction. AC-31 was the strongest antidiabetic compound compared to AC-51, AC-33 and cyclocommunolwithIC50values are 15.73, 24.41,49.49,and72.20μg/mL. Kineticstudies of AC-31 using Lineweaver-Burk method showed that inhibition mechanism of enzymeα-glucosidase was anon-competitivetype.

Keywords


Artocarpus altilis; antidiabetic; α-glucosidase; flavonoid

Full Text:

Full Text PDF


References

[1] Kasahara, S., and Hemmi, S., 1986, Medicinal Herb Index in Indonesia. P.T. Eisai Indonesia, Bogor, pp. 184.

[2] Wang, Y., Kedi, X., Lin, L., Pan, Y., and Zheng, X., 2007, Phytochemistry, 68 (9), 1300–1306.

[3] Nagappa, N.A., Thakurdesai, P.A., Venkat, R.N., and Singh, J., 2003, J. Ethnopharmacol., 88 (1), 45–50.

[4] Oh, W.K., Lee, C.H., Lee, M.S., Bae, E.Y., Sohn, C.B., Oh, H., Kim, B.Y., and Ahn, J.S., 2005, J. Ethnopharmacol., 96 (3), 411–415.

[5] Lotulung, P.D.N., Fajriah, S., Sundowo, A., and Filaila, E., 2009, Indo. J. Chem., 9 (3), 466–469.

[6] Gao, H., Nishioka, T., Kawabata, J., and Kasai, T., 2004, Biosci. Biotechnol. Biochem., 68 (2), 369–357.

[7] Dewi, R.T., Iskandar, Y., Hanafi, M., Kardono, L.B.S., Angelina, M., Dewijanti, D.I., and Banjarnahor, S.D.S., 2007, Pak. J. Biol. Sci., 10 (18), 3131–3135.

[8] Meng, P., and Zhou, X., 2012, Med. Chem.Res., 21 (12), 4422–4429.

[9] Annapurna, A., Kumar, V.K., Rao, N.K., Harish, G., and Kumar, K.V., 2001, Indian J. Pharm. Sci., 63, 273–278.

[10] Rao, N.K., 2006. BMC Complement. Altern. Med., 6 (17), 1–6.

[11] Iwai, K., 2008, Plant Foods Hum. Nutr., 63 (4), 163–169.

[12] Peyrieras, N., Bause, E., Legler, G., Vasilov, R., Claesson, L., Peterson, P., and Ploegh, H., 1983, EMBO J., 2 (6), 823–832.

[13] Göke, B., and Herrmann-Rinke, C., 1998, Diebetes Metab. Rev., 14 (S1), S31–S38.

[14] Kim, Y-M., Wang, M-H., and Rhee, H-I., 2004, Carbohydr. Res., 339 (3), 715–717.

[15] Jones, A.M.P., Ragone, D., Tavana, N.G., Bernotas, D.W., and Murch, S.J., 2011, Ethnobotany Res. Appl., 9, 129–149.

[16] Lotulung, P.D.N., 2007, Artocarpus Communis Leaves as Antidiabetic Phytopharmaca, Progress Report LIPI Competitive Project.

[17] Lotulung, P.D.N., Fajriah, S., Hanafi, M., and Filaila, E., 2008, Pak. J. Biol. Sci., 11 (2), 2517–2520.

[18] Lin, C-N., and Shieh, W-L., 1992, Phytochemistry, 31 (8), 2922–2924.

[19] Syah, Y.M., Achmad, S.A., Bakhtiar, E., Hakim, E.H., Juliawaty, L.D., and Latip, J., 2006, Jurnal Matematika dan Sains, 11 (3), 100–104.



DOI: https://doi.org/10.22146/ijc.21261

Article Metrics

Abstract views : 2845 | views : 3159


Copyright (c) 2014 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Web Analytics View The Statistics of Indones. J. Chem.