HPLC Fingerprint Analysis Combined with Chemometrics for Authentication of Kaempferia galanga from Related Species

https://doi.org/10.22146/ijc.21146

Cahya Septyanti(1), Irmanida Batubara(2*), Mohamad Rafi(3)

(1) Department of Chemistry, Faculty of Mathematic and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Kimia Building, Tanjung Street, Bogor 16680
(2) Department of Chemistry, Faculty of Mathematic and Natural Sciences, Bogor Agricultural University, Darmaga Campus, Kimia Building, Tanjung Street, Bogor 16680 Biopharmaca Research Center-Research and Community Empowerment Institute, Bogor Agricultural University, Bogor 16128
(3) Biopharmaca Research Center-Research and Community Empowerment Institute, Bogor Agricultural University, Bogor 16128
(*) Corresponding Author

Abstract


Fingerprint analysis using high performance liquid chromatography (HPLC) has been developed for authentication of Kaempferia galanga from related species, such as Kaempferia pandurata and K. rotunda. By comparing the fingerprint chromatograms of K. galanga, K. pandurata and K. rotunda, we could identify K. galanga samples and detect adulteration of K. galanga from K. pandurata and K. rotunda by using their marker peaks. We also combined HPLC fingerprint with chemometrics for discrimination the three species and also for authentication of K. galanga. All the three species and K. galanga adulterated with K. pandurata and K. rotunda were discriminated successfully by using principal component analysis (PCA) and discriminant analysis (DA). This result indicates that HPLC fingerprint analysis in combination with PCA (PC1 = 30.06% and PC2 = 34.74%) and DA (DF1 = 94.59% and DF2 = 3.32%) could be used for authentication of K. galanga samples from the related species.

Keywords


Kaempferia galanga; HPLC; Fingerprint analysis; Chemometrics

Full Text:

Full Text Pdf


References

[1] Lakshmanan, D., Werngren, J., Jose, L., Suja, K.P., Nair, M.S., Varma, R.L., Mundayoor, S., Hoffner, S., and Kumar, R.A., 2011, Fitoterapia, 82 (5), 757–761.

[2] Mekseepralard, C., Kamkaen, N., and Wilkinson, J.M., 2010, Phytother. Res., 24 (10), 1514–1519.

[3] Sutthanont, N., Choochote, W., Tuetun, B., Junkum, A., Jitpakdi, A., Chaithong, U., Riyong, D., and Pitasawat, B., 2010, J. Vector Ecol., 35 (1), 106–115.

[4] Ridtitid, W., Sae-wong, C., Reanmongkol, W., and Wongnawa, M., 2008, J. Ethnopharmacol., 118 (2), 225–230.

[5] Tewtrakul, S., and Subhadhirasakul, S., 2007, J. Ethnopharmacol., 109 (3), 535–538.

[6] Roemantyo, H.S., and Soekarman, 1996, Warta Tumbuhan Obat Indonesia, 3 (2), 15–16.

[7] Umar, M.I., Asmawi, M.Z., Sadikun, A., Atangwho, I.J., Yam, M.F., Altaf, R., and Ahmed, A., 2012, Molecules, 17 (7), 8720–8734.

[8] Liang, Y.Z., Xie, P., and Chan, K., 2004, J. Chromatogr. B, 812 (1-2), 53–70.

[9] Akarasereenont, P., Thitilertdecha, P., Chotewuttakorn, S., Palo, T., Seubnooch, P., Wattanarangsan, J., Phadungrakwitya, R., Klumklomijt, S., Chareonkij, P., Sathiyos, Y., and Laohapand, T., 2010, Siriraj Med. J., 62 (1), 4–8.

[10] Li, Y., Wu, T., Zhu, J., Wan, L., Yu, Q., Li, X., Cheng, Z., and Guo, C., 2010, J. Pharm. Biomed. Anal., 52 (4), 597–602.

[11] Zou, H., Yang, G., Du, A., Yuan, J., Qin, Z., Xia, Y., and Aboul-Enein, H.Y., 2006, Biomed. Chromatogr., 20 (6-7), 642–655.

[12] Zhao, Y., Zhang, Y., Lin, R., and Sun, W., 2009, Fitoterapia, 80 (6), 333–338.

[13] Rafi, M., Wulansari, L., Heryanto, R., Darusman, L.K., Lim, L.W., and Takeuchi, T., 2015, Food Anal. Methods, 8 (9), 2185–2193.

[14] Wang, L., Wang, X., and Kong, L., 2012, Biochem. Syst. Ecol., 40, 138–145.

[15] Yang, S.O., Lee, S.W., Kim, Y.O., Sohn, S.H., Kim, Y.C., Hyun, D.Y., Hong, Y.P., and Shin, Y.S., 2013, J. Gingseng Res., 37 (2), 248–253.

[16] Rafi, M., Lim, L.W., Takeuchi, T., and Darusman, L.K., 2013, Anal. Bioanal. Chem., 405 (20), 6599–6603.



DOI: https://doi.org/10.22146/ijc.21146

Article Metrics

Abstract views : 3287 | views : 3521


Copyright (c) 2016 Indonesian Journal of Chemistry

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

 

Indonesian Journal of Chemistry (ISSN 1411-9420 /e-ISSN 2460-1578) - Chemistry Department, Universitas Gadjah Mada, Indonesia.

Web Analytics View The Statistics of Indones. J. Chem.