Induced-Coagulated Plasma-Fibrin Gels as a Biological Scaffold for Cell Attachment and Proliferation of Umbilical Cord-Derived Mesenchymal Stem Cells (UC-MSC)
https://doi.org/10.22146/ijbiotech.9310 Rio Hermantara(1), Fiano A. Kerans(2), Rizal R(3), E. Henny Herningtyas(4*), Lutfan Lazuardi(5)
(1)
(2) Biotechnology Study Program, The Graduate Program Universitas Gadjah Mada, Yogyakarta, Indonesia
(3) Biotechnology Study Program, The Graduate Program Universitas Gadjah Mada, Yogyakarta, Indonesia
(4) Faculty of Medicine Universitas Gadjah Mada, Yogyakarta, Indonesia
(5) 1 Biotechnology Study Program, The Graduate Program Universitas Gadjah Mada, Yogyakarta, Indonesia 2 Faculty of Medicine Universitas Gadjah Mada, Yogyakarta, Indonesia
(*) Corresponding Author
Abstract
Fibrin gels are an ideal natural biological scaffold for tissue engineering because they are biocompatible,
biodegradable, and have many biological surface markers. However, most research on fi brin gels used commercial
fi brin kits that could be costly and limited in some areas. In this study, fi brin gels were made by inducing blood
coagulation by adding a common diagnostic kit to assess the time for blood to clot, called activated partial
thromboplastin time (aPTT). This induced coagulated plasma (iCoplas)-fi brin gels was evaluated for its ability to
enhance biological activity of umbilical cord-derived mesenchymal stem cell (UC-MSC), which were cell attachment
and proliferation. Fibrinogen concentration had infl uence on cell attachment, where only 50% of the cells could
attach to 77 mg/dl fi brinogen gels whereas 93% cells adhered to 154 mg/dl fi brin gels. There were no signifi cant
differences in cell proliferation on polysterene culture dish and fi brin gels (p>0.05). These results showed that
iCoplas-fi brin gels could be used as a fi brin-based scaffold, yielding no signifi cant difference than polysterene-tissue
culture dish cultures in cell attachment and cell proliferation on 154 mg/dl fi brinogen concentration.
biodegradable, and have many biological surface markers. However, most research on fi brin gels used commercial
fi brin kits that could be costly and limited in some areas. In this study, fi brin gels were made by inducing blood
coagulation by adding a common diagnostic kit to assess the time for blood to clot, called activated partial
thromboplastin time (aPTT). This induced coagulated plasma (iCoplas)-fi brin gels was evaluated for its ability to
enhance biological activity of umbilical cord-derived mesenchymal stem cell (UC-MSC), which were cell attachment
and proliferation. Fibrinogen concentration had infl uence on cell attachment, where only 50% of the cells could
attach to 77 mg/dl fi brinogen gels whereas 93% cells adhered to 154 mg/dl fi brin gels. There were no signifi cant
differences in cell proliferation on polysterene culture dish and fi brin gels (p>0.05). These results showed that
iCoplas-fi brin gels could be used as a fi brin-based scaffold, yielding no signifi cant difference than polysterene-tissue
culture dish cultures in cell attachment and cell proliferation on 154 mg/dl fi brinogen concentration.
Keywords
Fibrin gels, mesenchymal stem cells, activated partial thromboplastin time, cell attachment and proliferation.
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PDF DOI: https://doi.org/10.22146/ijbiotech.9310
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