Effects of Light Quality on Vegetative Growth and Flower Initiation in Phalaenopsis
https://doi.org/10.22146/ijbiotech.8632 Kumala Dewi(1*), Yekti Asih Purwestri(2), Yohana Theresia Maria Astuti(3), Lila Natasaputra(4), P. Parmi(5)
(1) Universitas Gadjah Mada
(2) Universitas Gadjah Mada
(3) Instiper
(4)
(5) Universitas Gadjah Mada
(*) Corresponding Author
Abstract
The effects of LEDs (Light-Emitting Diodes) emitting different colours namely red, blue, red and
blue, and white lights on vegetative growth and fl ower initiation of Phalaenopsis have been evaluated.
Phalaenopsis“otohine/taisuco fi re bird” seedlings in vitro were subjected to different light qualities for either
2 or 4 weeks, and then each seedling was planted in a plastic pot containing sphagnum and grown in the
growth chamber under similar light quality for 3 months. For fl ower induction, mature Phalaenopsis plants
having 4 – 6 leaves were grown for 3 months in the growth chamber under different light qualities. The leaf
span, chlorophyll, gibberellin and cytokinin content were determined. In addition, the expressions of FT-like
gene in the leaf, axillary bud, fl ower bud and stalk were examined.
Vegetative growth was enhanced under blue, red-blue or white LEDs compared to that of the control.
Gibberellin and cytokinin content increased in the seedlings subjected to white LEDs. Based on the average
of leaf span increment it was suggested that the growth of Phalaenopsis seedlings can be promoted by giving
either blue, red-blue or white LEDs. From the second experiment, it was found that fl ower induction in
Phalaenopsis can be obtained in plants that had just fi nished fl owering without the application of LEDs. The
expression of FT-like gene in the leaf as well as fl ower bud and stalk suggests that this gene is involved in
fl ower regulation of Phalaenopsis.
blue, and white lights on vegetative growth and fl ower initiation of Phalaenopsis have been evaluated.
Phalaenopsis“otohine/taisuco fi re bird” seedlings in vitro were subjected to different light qualities for either
2 or 4 weeks, and then each seedling was planted in a plastic pot containing sphagnum and grown in the
growth chamber under similar light quality for 3 months. For fl ower induction, mature Phalaenopsis plants
having 4 – 6 leaves were grown for 3 months in the growth chamber under different light qualities. The leaf
span, chlorophyll, gibberellin and cytokinin content were determined. In addition, the expressions of FT-like
gene in the leaf, axillary bud, fl ower bud and stalk were examined.
Vegetative growth was enhanced under blue, red-blue or white LEDs compared to that of the control.
Gibberellin and cytokinin content increased in the seedlings subjected to white LEDs. Based on the average
of leaf span increment it was suggested that the growth of Phalaenopsis seedlings can be promoted by giving
either blue, red-blue or white LEDs. From the second experiment, it was found that fl ower induction in
Phalaenopsis can be obtained in plants that had just fi nished fl owering without the application of LEDs. The
expression of FT-like gene in the leaf as well as fl ower bud and stalk suggests that this gene is involved in
fl ower regulation of Phalaenopsis.
Keywords
Light Emitting Diodes (LEDs); Phalaenopsis; gibberellin; cytokinin
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PDF DOI: https://doi.org/10.22146/ijbiotech.8632
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