Manufacturing and characterization of an effective and eco‐friendly nano insecticide with the aid of green alga Chlorella vulgaris in contrast to traditional insecticide

Rasha Sattam Hameed; Raghad Jasim Fayyad; Maan Abdul Azeez Shafeeq; Rasha Saad Nuaman

doi:10.22146/ijbiotech.79105

Manufacturing and characterization of an effective and eco‐friendly nano insecticide with the aid of green alga Chlorella vulgaris in contrast to traditional insecticide

https://doi.org/10.22146/ijbiotech.79105

Rasha Sattam Hameed⁽¹⁾, Raghad Jasim Fayyad⁽²⁾, Maan Abdul Azeez Shafeeq^(3*), Rasha Saad Nuaman⁽⁴⁾

(1) Department of Biology, Collage of Science, Mustansiriyah University, Baghdad, Iraq
(2) Department of Biology, Collage of Science, Mustansiriyah University, Baghdad, Iraq
(3) Department of Biology, Collage of Science, Mustansiriyah University, Baghdad, Iraq
(4) Department of Biology, Collage of Science, Mustansiriyah University, Baghdad, Iraq
(*) Corresponding Author

Abstract

There is a growing demand for the manufacture of eco‐friendly insecticide. This study aimed to establish an aqueous extract of Chlorella vulgaris as a green factory to manufacture nano‐insecticide of titanium nanoparticles to control house flies (Musca domestica) by describing the basic properties of TiO₂ solution before and after manufacturing. The absorbance was raised to 0.58, while transmission decreased to 38 under UV‐Visible spectra. Regarding to XRD analysis, seven sharp diffraction peaks appeared for a bulk solution while only three sharp peaks were noticed after phyco‐based synthesis. The crystal size of the prepared titanium nanoparticles was determined to be 27.39 nm. Furthermore, the observed size for bulk particles ranged from 92.33 to 249.6 nm through SEM, while for nanocrystalline the size ranged from 9.395 to 206 nm. Various phytochemicals were detected within the algal extract, including phenols, tannins, alkaloids, flavonoids, resins, and saponins. All of these active compounds participated in nano‐synthesis by acting as reducing and stabilizing agents. Finally, titanium nanoparticles were used as a controlling agent against house flies Musca domestica. In this study, this nanoparticles application also has been compared with traditional insecticide Imidacloprid. The high mortality percentages reached 100% against the first larval stage, 70% against the third larval stage, and 93.3% in adult flies. These mortalities were higher after using Imidacloprid for all tested stages. Many phenotypic distortions were also observed in house flies treated with TiO₂ NPs prepared by Chlorella, including failure in pupal emergence and maturity, incomplete development in the head, legs, and wings, and disappearance of the genital organs. The study demonstrated that C. vulgaris is a good candidate for nanomanufacturing and a rich naturally derived nanopesticide.

Keywords

Alkaloids; Chlorophytes; Dextran; Emergence; House fly; Stabilizing agents

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References

Abdel-Rahim E, Mohamed S. 2013. Comparative toxic activity of four algae, against the 2nd and 4th larval instars of black cutworm, Agrotis ipsilon (Hufnagel). Egypt. J. Agric. Res 91(4):1303–1318. doi:10.21608/ejar.2013.165113.

Antić Ž, Krsmanović RM, Nikolić MG, Marinović- Cincović M, Mitrić M, Polizzi S, Dramićanin MD. 2012. Multisite luminescence of rare earth doped TiO2 anatase nanoparticles. Mater. Chem. Phys. 135(2-3):1064–1069. doi:10.1016/j.matchemphys.2012.06.016.

Bagheri S, Shameli K, Abd Hamid SB. 2013. Synthesis and characterization of anatase titanium dioxide nanoparticles using egg white solution via Sol-Gel method. J. Chem. 2013:848205. doi:10.1155/2013/848205.

Brun E, Barreau F, Veronesi G, Fayard B, Sorieul S, Chanéac C, Carapito C, Rabilloud T, Mabondzo A, Herlin-Boime N, Carrière M. 2014. Titanium dioxide nanoparticle impact and translocation through ex vivo, in vivo and in vitro gut epithelia. Part. Fibre Toxicol. 11(1):1–16. doi:10.1186/1743-8977-11-13.

De Oliveira EM, Da Rocha MS, Froner APP, Basso NR, Zanini ML, Papaléo RM. 2019. Synthesis and nuclear magnetic relaxation properties of composite iron oxide nanoparticles. Quim. Nova 42(1):57–64. doi:10.21577/0100-4042.20170309.

Guerrero A, Malo E, Coll-Toledano J, Quero C. 2014. Semiochemical and natural product-based approaches to control Spodoptera spp. (Lepidoptera: Noctuidae). J. Pest. Sci. 87:231–247. doi:10.1007/s10340-013- 0533-7.

Gutiérrez-Ramírez JA, Betancourt-Galindo R, AguirreUribe LA, Cerna-Chávez E, Sandoval-Rangel A, Ángel ECD, Chacón-Hernández JC, García-López JI, Hernández-Juárez A. 2021. Insecticidal effect of zinc oxide and titanium dioxide nanoparticles against Bactericera cockerelli Sulc. (hemiptera: Triozidae) on tomato Solanum lycopersicum. Agronomy 11(8):1460. doi:10.3390/agronomy11081460.

Hameed RS, Fayyad RJ, Nuaman RS, Hamdan NT, Maliki SA. 2019. Synthesis and characterization of a novel titanium nanoparticals using banana peel extract and investigate its antibacterial and insecticidal activity. J. Pure Appl. Microbiol. 13(4):2241–2249. doi:10.22207/JPAM.13.4.38.

Nadeem M, Tungmunnithum D, Hano C, Abbasi BH, Hashmi SS, Ahmad W, Zahir A. 2018. The current trends in the green syntheses of titanium oxide nanoparticles and their applications. Green Chem. Lett. Rev. 11(4):492–502. doi:10.1080/17518253.2018.1538430.

Negi S, Singh V. 2018. Algae: A potential source for nanoparticle synthesis. J. Appl. Nat. Sci. 10(4):1134– 1140. doi:10.31018/jans.v10i4.1878.

Paramo LA, Feregrino-Pérez AA, Guevara R, Mendoza S, Esquivel K. 2020. Nanoparticles in agroindustry: Applications, toxicity, challenges, and trends. Nanomaterials 10(9):1654. doi:10.3390/nano10091654.

Rosales-Mendoza S, García-Silva I, González-Ortega O, Sandoval-Vargas JM, Malla A, Vimolmangkang S. 2020. The Potential of Algal Biotechnology to Produce Antiviral Compounds and Biopharmaceuticals. Molecules 25(18):4049. doi:10.3390/molecules25184049.

Sabat D, Patnaik A, Ekka B, Dash P, Mishra M. 2016. Investigation of titania nanoparticles on behaviour and mechanosensory organ of Drosophila melanogaster. Physiol. Behav. 167:76–85. doi:10.1016/j.physbeh.2016.08.032.

Saber AA, Hamed SM, Abdel-Rahim EF, Cantonati M. 2018. Insecticidal prospects of algal and cyanobacterial extracts against the cotton leafworm Spodoptera littoralis. Vie Milieu 68(4):199–212. Shah SNA, Shah Z, Hussain M, Khan M. 2017. Hazardous Effects of Titanium Dioxide Nanoparticles in Ecosystem. Bioinorg. Chem. Appl. 2017:4101735. doi:10.1155/2017/4101735.

Shaikh JR, Patil M. 2020. Qualitative tests for preliminary phytochemical screening: An overview. Int. J. Chem. Stud. 8(2):603–608. doi:10.22271/chemi.2020.v8.i2i.8834.

Shaker AM, Zaki AH, Abdel-Rahim EF, Khedr MH. 2017. TiO2 nanoparticles as an effective nanopesticide for cotton leaf worm. Agric. Eng. Int. CIGR J. 19:61–68. Sharma A, Sharma S, Sharma K, Chetri SP, Vashishtha A, Singh P, Kumar R, Rathi B, Agrawal V. 2016. Algae as crucial organisms in advancing nanotechnology: a systematic review. J. Appl. Phycol. 28(3):1759–1774. doi:10.1007/s10811-015-0715-1.

Sinha SN, Paul D, Halder N, Sengupta D, Patra SK. 2015. Green synthesis of silver nanoparticles using fresh water green alga Pithophora oedogonia (Mont.) Wittrock and evaluation of their antibacterial activity. Appl. Nanosci. 5(6):703–709. doi:10.1007/s13204- 014-0366-6.

Sugeçti S, Tunçsoy B, Büyükgüzel E, Özalp P, Büyükgüzel K. 2021. Ecotoxicological effects of dietary titanium dioxide nanoparticles on metabolic and biochemical parameters of model organism Galleria mellonella (Lepidoptera: Pyralidae). J. Environ. Sci. Health. C. Toxicol. Carcinog. 39(4):423–434. doi:10.1080/26896583.2021.1969846.

Torabfam M, Yüce M. 2020. Microwave-assisted green synthesis of silver nanoparticles using dried extracts of Chlorella vulgaris and antibacterial activity studies. Green Process. Synth. 9(1):283–293. doi:10.1515/gps-2020-0024.

Usharani G, Srinivasan G, Sivasakthi S, P S. 2015. Antimicrobial Activity of Spirulina platensis Solvent Extracts Against Pathogenic Bacteria and Fungi. Adv. Biol. Res. 9(5):292–298. doi:10.5829/idosi.abr.2015.9.5.9610.

DOI: https://doi.org/10.22146/ijbiotech.79105