Expression Analysis and Nuclear Import Study of Full-length Isoforms Importin α as 6x Histidin-tagged Fusion Protein on the Intracellular Localization of Recombinant HBV Core Protein
Aris Haryanto(1*)
(1) Department of Biochemistry, Faculty of Veterinary Medicine, Gadjah Mada University, Yogyakarta 55281
(*) Corresponding Author
Abstract
Keywords
Full Text:
PDFReferences
Ben-Efraim, I. and Gerace, L., 2001. Gradient of increasing affinity of importin b for nucleoporins along the pathway of nuclear import. J. Cell. Biol., 152, 411- 418.
Chelsky, D., Ralph, R. and Jonak, G., 1989. Sequence requirements for synthetic peptide-mediated translocation to the nucleus. Mol. Cell. Biol., 9, 2487-2492.
Conti, E., Uy, M., Leighton, L., Blobel, G. and Kriyan, J., 1998. Crytallographic analysis of the recognition a nuclear localization signal by the nuclear import factor karyopherin alpha. Cell, 94, 193-104.
Conti, E. and Kuriyan, J., 2000. Crystallographic and lysis of specific yet versatile recognition of distint nuclear localization signal by karyopherin alpha. Structure, 8, 329-338.
Cuomo, C.A., Kirch, S.A., Gyuris, J., Bread, R. and Oettinger, M.A., 1994. Rch1, a protein that specifially interacts with the RAG-1 recombination activating protein. Proc. Natl. Acad. Sci., 91, 6156- 6160.
Dang, C.V. and Lee, W.H., 1989. Nuclear and nucleolar targeting sequences of c-erb A, c-myb, n-myc, p53, HSP70 and HIV tat proteins. J. Biol. Chem., 264, 18019- 18023.
Fried, H. and Kutay, U., 2003. Nucleocytoplasmic transport: taking an inventory. Cell. Mol. Life. Sci., 60, 1659-1688.
Görlich, D., Prehn, S., Laskey, R.A. and Hartmann, E., 1994. Isolation of a protein that is essensial for the first step of nuclear protein import. Cell 79, 767- 778.
Görlich, D., Vogel, F., Mills, A.D., Hartmann, E. and Laskey, R.A., 1995. Distinct functions for the two importin subunit in nuclear protein import. Nature, 377, 246-248.
Görlich, D., Henklein, P., Laskey, R.A. and Hartmann E., 1996. A 41 amino acid motif in importin alpha confers binding to importin beta and hence transit into the nucleus. EMBO J., 15, 1810-1817.
Görlich, D. and Kutay, U. 1999. Transport between the cell nucleus and the cytoplasm. Annu. Rev. Cell. Dev. Biol., 15, 607-660.
Haryanto, A., 2004. Generation and expression of recombinant glutathione- S-transferase full-length importin a-3 (GST-importin a-3) fusion protein and study of its role on the nuclear transport of protein. I. J. Biotech., ed. June. 681- 720.
Herold, A., Truant, R., Wiegand, H. and Cullens, B.R., 1998. Determination of the functional domain organisation of the importin alpha nuclear import factor. J. Cell. Biol., 143, 309-318.
Kobe, B., 1999. Autoinhibition by an internal nuclear localization signal revealed by the crystal structure of mammalian importin alpha. Nat. Struct. Biol., 6, 388- 397.
Köhler, M., Ansieau, S., Prehn, S., Leutz, A., Haller, H. and Hartmann, E., 1997. Cloning of two novel importin a subunit and analysis of the expression pattern of the importin a protein family. FEBS lett., 417, 104-108.
Lee, S.J., Matsuura, Y., Liu, S.M. and Stewart, M. 2005. Structural basis for nuclear import complex dissociation by RanGTP. Nature, 435 (7042), 693-696.
Makkerh, J.P., Dingwall, C. and Laskey, R.A. 1996. Comparative mutagenesis of nuclear localization signals reveals the
DOI: https://doi.org/10.22146/ijbiotech.7412
Article Metrics
Abstract views : 1459 | views : 1510Refbacks
- There are currently no refbacks.
Copyright (c) 2015 Indonesian Journal of Biotechnology