The expression of growth factor signaling genes in co-culture IVM

Erif Maha Nugraha Setiawan; Hyun Ju Oh; Min Jung Kim; Geon A Kim; Seok Hee Lee; Yoo Bin Choi; Ki Hae Ra; Byeong Chun Lee

doi:10.22146/ijbiotech.27309

The expression of growth factor signaling genes in co-culture IVM

https://doi.org/10.22146/ijbiotech.27309

Erif Maha Nugraha Setiawan^(1*), Hyun Ju Oh⁽²⁾, Min Jung Kim⁽³⁾, Geon A Kim⁽⁴⁾, Seok Hee Lee⁽⁵⁾, Yoo Bin Choi⁽⁶⁾, Ki Hae Ra⁽⁷⁾, Byeong Chun Lee⁽⁸⁾

(1) Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea
(2) Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea
(3) Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea
(4) Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea
(5) Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea
(6) Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea
(7) Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea
(8) Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Seoul National University, 1 Gwanak-ro, Gwanak-gu, Seoul 08826, Republic of Korea
(*) Corresponding Author

Abstract

The objective of this study was to determine the expression of growth factor signaling genes in human adiposederived stem cells (ASCs), porcine oocytes, and cumulus during in vitro maturation (IVM). The human ASCs (from 2 young and 2 old donors) were used for the co-culture IVM system. The maturation rate was examined based on polar body extrusion. The expression of the growth factor signaling genes from ASCs, oocytes, and cumulus were measured using qPCR. All data were analyzed using ANOVA followed by Tukey’s test. The expression of the h-IGF1 signaling genes from human ASCs cells showed similar values in all groups and the h-FGF2 expressions were higher in the young donors than the old ones. The p-FGF2, p-FGFR2, and p-TGFβ1 expressions in the oocytes as well as p-IGFR in the cumulus that were co-cultured from the young donors showed higher values than the old and control groups. The apoptotic ratio (p-BAX/p-BCL2) from the oocytes and cumulus in both co-culture groups also showed lower levels than the control (P<0.05). Oocyte maturation rates were significantly increased in all co-cultured groups (Y1 (85.9 ± 2.2%), Y2 (91.2 ± 1.1%), O1 (86.3 ± 1.5%), and O2 (86.5 ± 2.3%)) compared with the control (76.7 ± 1.1%; P<0.05). Although the expression of growth factor signaling genes was varied, young donors’ ASCs might support in vitro maturation beħer than those from old donors.

Keywords

co-culture; gene expression; human ASCs; oocytes maturation

Full Text:

PDF

References

Appeltant R, Somfai T, Kikuchi K, Maes D, Van Soom A. 2016. Influence of co-culture with denuded oocytes during in vitro maturation on fertilization and developmental competence of cumulus-enclosed porcine oocytes in a defined system: co-culture with denuded oocytes. Animal Science Journal 87:503–510. doi:10.1111/asj.12459.

Appeltant R, Somfai T, Nakai M, Bodó S, Maes D, Kikuchi K, Van Soom A. 2015. Interactions between oocytes and cumulus cells during in vitro maturation of porcine cumulus-oocyte complexes in a chemically defined medium: effect of denuded oocytes on cumulus expansion and oocyte maturation. Theriogenology 83:567–576. doi:10.1016/j.theriogenology.2014.10.026.

Arat S, Caputcu AT, Cevik M, Akkoc T, Cetinkaya G, Bagis H. 2016. Effect of growth factors on oocyte maturation and allocations of inner cell mass and trophectoderm cells of cloned bovine embryos. Zygote 24:554–562. doi:10.1017/S0967199415000519.

Bentov Y, Werner H. 2011. IGF1R (insulin-like growth factor 1 receptor). Atlas of Genetics and Cytogenetics in Oncology and Haematology. doi:10.4267/2042/44533.

Bogacki M, Wasielak M, Kitewska A, Bogacka I, Jalali B. 2014. The effect of hormonal estrus induction on maternal effect and apoptosis-related genes expression in porcine cumulus-oocyte complexes. Reproductive Biology and Endocrinology 12:32. doi:10.1186/1477-7827-12-32.

Coticchio G, Dal Canto M, Mignini Renzini M, Guglielmo MC, Brambillasca F, Turchi D, Novara PV, Fadini R. 2015. Oocyte maturation: gamete-somatic cells interactions, meiotic resumption, cytoskeletal dynamics and cytoplasmic reorganization. Human Reproduction Update 21:427–454. doi:10.1093/humupd/dmv011.

da Silva Meirelles L, Fontes AM, Covas DT, Caplan AI. 2009. Mechanisms involved in the therapeutic properties of mesenchymal stem cells. Cytokine & Growth Factor Reviews 20:419–427. doi:10.1016/j.cytogfr.2009.10.002.

da Silveira JC, Carnevale EM, Winger QA, Bouma GJ. 2014. Regulation of ACVR1 and ID2 by cell-secreted exosomes during follicle maturation in the mare. Reproductive Biology and Endocrinology 12:44. doi:10.1186/1477-7827-12-44.

Fu X, He Y, Xie C, Liu W. 2008. Bone marrow mesenchymal stem cell transplantation improves ovarian function and structure in rats with chemotherapy-induced ovarian damage. Cytotherapy 10:353–363. doi:10.1080/14653240802035926.

Gilchrist RB. 2011. Recent insights into oocyte - follicle cell interactions provide opportunities for the development of new approaches to in vitro maturation. Reproduction, Fertility and Development 23:23. doi:10.1071/RD10225.

Hull KL, Harvey S. 2014. Growth hormone and reproduction: a review of endocrine and autocrine/paracrine interactions. International Journal of Endocrinology 2014:1–24. doi:10.1155/2014/234014.

Jin JX, Lee S, Khoirinaya C, Oh A, Kim GA, Lee BC. 2016. Supplementation with spermine during in vitro maturation of porcine oocytes improves early embryonic development after parthenogenetic activation and somatic cell nuclear transfer. Journal of Animal Science 94:963–970. doi:10.2527/jas.2015-9761.

Kranc W, Budna J, Chachuła A, Borys S, Bryja A, Rybska M, Ciesiółka S, Sumelka E, Jeseta M, Brüssow KP, et al. 2017. “Cell migration” is the ontology group differentially expressed in porcine oocytes before and after in vitro maturation: a microarray approach. DNA and Cell Biology 36:273–282. doi:10.1089/dna.2016.3425.

Machtinger R, Laurent LC, Baccarelli AA. 2016. Extracellular vesicles: roles in gamete maturation, fertilization and embryo implantation. Human Reproduction Update 22:182–193. doi:10.1093/humupd/dmv055.

Overman JR, Helder MN, ten Bruggenkate CM, Schulten EAJM, Klein-Nulend J, Bakker AD. 2013. Growth factor gene expression profiles of bone morphogenetic protein-2-treated human adipose stem cells seeded on calcium phosphate scaffolds in vitro. Biochimie 95:2304–2313. doi:10.1016/j.biochi.2013.08.034.

Pandey AC, Semon JA, Kaushal D, O’Sullivan RP, Glowacki J, Gimble JM, Bunnell BA. 2011. MicroRNA profiling reveals age-dependent differential expression of nuclear factor κB and mitogen-activated protein kinase in adipose and bone marrow-derived human mesenchymal stem cells. Stem Cell Res Ther 2:49. doi:10.1186/scrt90.

Pereira GR, Lorenzo PL, Carneiro GF, Ball BA, Bilodeau-Goeseels S, Kastelic J, Pegoraro LMC, Pimentel CA, Esteller-Vico A, Illera JC, et al. 2013. The involvement of growth hormone in equine oocyte maturation, receptor localization and steroid production by cumulus–oocyte complexes in vitro. Research in Veterinary Science 95:667–674. doi:10.1016/j.rvsc.2013.06.024.

Pomini Pinto RF, Fontes PK, Loureiro B, Sousa Castilho AC, Sousa Ticianelli J, Montanari Razza E, Satrapa RA, Buratini J, Moraes Barros C. 2015. Effects of FGF10 on bovine oocyte meiosis progression, apoptosis, embryo development and relative abundance of developmentally important genes in vitro. Reproduction in Domestic Animals 50:84–90. doi:10.1111/rda.12452.

Ra JC, Shin IS, Kim SH, Kang SK, Kang BC, Lee HY, Kim YJ, Jo JY, Yoon EJ, Choi HJ, et al. 2011. Safety of intravenous infusion of human adipose tissue-derived mesenchymal stem cells in animals and humans. Stem Cells and Development 20:1297–1308. doi:10.1089/scd.2010.0466.

Saadeldin IM, Kim SJ, Choi YB, Lee BC. 2014. Improvement of cloned embryos development by co-culturing with parthenotes: a possible role of exosomes/microvesicles for embryos paracrine communication. Cell Reprogram 16:223–234. doi:10.1089/cell.2014.0003.

Scruggs BA, Semon JA, Zhang X, Zhang S, Bowles AC, Pandey AC, Imhof KMP, Kalueff AV, Gimble JM, Bunnell BA. 2013. Age of the donor reduces the ability of human adipose-derived stem cells to alleviate symptoms in the experimental autoimmune encephalomyelitis mouse model. Stem Cells Translational Medicine 2:797–807. doi:10.5966/sctm.2013-0026.

Setyawan EMN, Kim MJ, Oh HJ, Kim GA, Jo YK, Lee SH, Choi YB, Lee BC. 2016. Spermine reduces reactive oxygen species levels and decreases cryocapacitation in canine sperm cryopreservation. Biochemical and Biophysical Research Communications 479:927–932. doi:10.1016/j.bbrc.2016.08.091.

Shimada M, Hernandez-Gonzalez I, Gonzalez-Robayna I, Richards JS. 2006. Paracrine and autocrine regulation of epidermal growth factor-like factors in cumulus oocyte complexes and granulosa cells: key roles for prostaglandin synthase 2 and progesterone receptor. Molecular Endocrinology 20:1352–1365. doi:10.1210/me.2005-0504.

Son Y-J, Lee S-E, Hyun H, Shin M-Y, Park Y-G, Jeong S-G, Kim E-Y, Park S-P. 2017. Fibroblast growth factor 10 markedly improves in vitro maturation of porcine cumulus-oocyte complexes. Molecular Reproduction and Development 84:67–75. doi:10.1002/mrd.22756.

Toori MA, Mosavi E, Nikseresht M, Barmak MJ, Mahmoudi R. 2014. Influence of Insulin-Like Growth Factor-I on Maturation and Fertilization Rate of Immature Oocyte and Embryo Development in NMRI Mouse with TCM199 and α-MEM Medium. Journal of Clinical and Diagnostic Research 8:AC05-08. doi:10.7860/JCDR/2014/9129.5242.

Uzumcu M, Pan Z, Chu Y, Kuhn PE, Zachow R. 2006. Immunolocalization of the hepatocyte growth factor (HGF) system in the rat ovary and the anti-apoptotic effect of HGF in rat ovarian granulosa cells in vitro. Reproduction 132:291–299. doi:10.1530/rep.1.00989.

Wilson A, Shehadeh LA, Yu H, Webster KA. 2010. Age-related molecular genetic changes of murine bone marrow mesenchymal stem cells. BMC Genomics 11:229. doi:10.1186/1471-2164-11-229.

Woods DC, Tilly JL. 2012. The next (re)generation of ovarian biology and fertility in women: is current science tomorrow’s practice? Fertility and Sterility 98:3–10. doi:10.1016/j.fertnstert.2012.05.005.

Wu W, Niklason L, Steinbacher DM. 2013. the effect of age on human adipose-derived stem cells. Plastic and Reconstructive Surgery 131:27. doi:10.1097/PRS.0b013e3182729cfc.

DOI: https://doi.org/10.22146/ijbiotech.27309