The growth improvement of Grammatophyllum scriptum (Lindl.) Bl. in vitro plantlet using photoautotrophic micropropagation system

Aries Bagus Sasongko; Asruwaidah Fatumi; Ari Indrianto

doi:10.22146/ijbiotech.27167

The growth improvement of Grammatophyllum scriptum (Lindl.) Bl. in vitro plantlet using photoautotrophic micropropagation system

https://doi.org/10.22146/ijbiotech.27167

Aries Bagus Sasongko^(1*), Asruwaidah Fatumi⁽²⁾, Ari Indrianto⁽³⁾

(1) Laboratory of Biotechnology, Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Sekip Utara, Yogyakarta 55281
(2) Laboratory of Biotechnology, Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Sekip Utara, Yogyakarta 55281
(3) Laboratory of Biotechnology, Faculty of Biology, Universitas Gadjah Mada, Jalan Teknika Selatan, Sekip Utara, Yogyakarta 55281
(*) Corresponding Author

Abstract

To improve the growth of Grammatophyllum scriptum (Lindl.) Bl. in vitro plantlet, a photoautotrophic micropropagation system (PMS) was developed by growing in vitro plantlet on VW medium with varying concentration of sucrose (0, 5, 10, and 20 g/L) and additional carbon dioxide from the air (bottle covered with cap or filter). The result showed that the leaf length would increase up to 6.5 cm with PMS and it would keep growing by the adding of 5 g/L sucrose. Average number of leaves increased by 6.7 strands with PMS and the addition of sucrose increased the average quantity of leaves up to 7.7 strands. Average number and root length would increase with PMS and would even increase more with 5 g/L sucrose addition. PMS with 5 g/L sucrose can increase chlorophyll a and b concentration. The number of stomata per unit area in PMS was lower than closed culture. This shows that PMS can increase the growth of G. scriptum in vitro plantlet and the growth increase would be effective if it is combined with sucrose addition.

Keywords

plant growth; Grammatophyllym scriptum plantlet; photoautotrophic micropropagation system; in vitro; carbon dioxide

Full Text:

PDF

References

Arditi, J. 2008. Micropropagation of Orchid 2^nd edition Volume 1. Blackwell Publishing: Oxford UK

Campbell, N.A., J.B. Reece and L.G. Mitchell. 2004. Biologi. Edisi kelima. Jilid 1.Erlangga. Jakarta. hal: 302-310.

Haney, R.L., W.F. Brinton., and E. Evans. (2008) Soil CO2 respiration: comparison of Chemical titration, CO2 irga analysis and the solvita gel system. Renewable Agriculture and Food Systems. 23(2): 171–176

Hani, M.R.,(2015) Pengaruh penyemprotan kitosan pada aklimatisasi planlet anggrek macan (Grammatophyllum scriptum). Jurusan Biologi Fakultas MIPA UNS. Surakarta.

Irawati.(2001) Dendrobium capra J.J. Sm. Dalam: Mogea, J.P., D. Gandawijdjaja, H. Wiriadinata, R.E. Nasution dan Irawati (ed.).Tumbuhan Langka Indonesia. Puslitbio, LIPI: Bogor.

Rosdiana. (2010) Pertumbuhan anggrek bulan (Phalaenopsis amboinensis) endemik Sulawesi, pada beberapa jenis dan konsentrasi zat pengatur tumbuh secara in vitro. Jurnal Agrisistem 6(2).

Sasmitamihardja D dan A. Siregar.1997. Fisiologi tumbuhan. Jurusan Biologi FMIPA – ITB. Proyek Pendidikan Tenaga Akademik Direktorat JenderalPendidikan dan Kebudayaan, Bandung.

Suprapti, E. (2005) Kadar klorofil dan karotenoid daun kentang (Solanum tuberosum L) yang ditanam pada jarak lokasi penanaman berbeda darisumber sulfur kawah Sikidang Dieng. Universitas Diponegoro.Semarang. hal: 6-31

Soeryowinoto, M.1987. Mengenal anggrek alam Indonesia. PT Penebar Swadaya: Jakarta.

Teixera da Silva1, J.A., Dam, D.T., Giang, and Michia, T., (2005) In vitro acclimatization of banana and Cymbidium. In International Journal of Botany 1(1): 41-49

Teixera da Silva2, J.A., Dam, D.T., Giang, and Michia, T., (2005) Effective acclimatization of Epidendrum in vitro using a novel micropropagation vessel. In Biotechnology 4(3): 214-220

Xiao, Y. and T.Kozai. (2006) In vitro multiplication of statice planletsusing sugar-free media. Scientia Holticulturae.109: 71-77.

Yulia, N. D. dan N. S. Ruseani. (2008) Studi habitat dan inventarisasi Dendrobium capra J.J. Smith di Kabupaten Madiun dan Bojonegoro. Biodiversitas.9: 190-193.

Zots, G. and M.T. Tyree. (1996) Water stress in the epiphytic orchid, Dimerandra emarginata (G,Meyer) Hoehne.Oecologia 107: 151-159.

DOI: https://doi.org/10.22146/ijbiotech.27167