Bioactivity and genetic screening of marine actinobacteria associated with red algae Gelidiella acerosa

Maria Ulfah; Noer Kasanah; Niken Satiti Nur Handayani

doi:10.22146/ijbiotech.25920

Bioactivity and genetic screening of marine actinobacteria associated with red algae Gelidiella acerosa

https://doi.org/10.22146/ijbiotech.25920

Maria Ulfah⁽¹⁾, Noer Kasanah^(2*), Niken Satiti Nur Handayani⁽³⁾

(1) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sinduadi, Mlati, Sleman, Daerah Istimewa Yogyakarta 55281, Indonesia
(2) Department of Fisheries, Faculty of Agriculture, Universitas Gadjah Mada, Bulaksumur, Caturtunggal, Depok, Sleman, Daerah Istimewa Yogyakarta 55281 Indonesia
(3) Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sinduadi, Mlati, Sleman, Daerah Istimewa Yogyakarta 55281, Indonesia
(*) Corresponding Author

Abstract

Bacterial resistance to existing antibiotics has driven a search for new antibiotics from marine actinobacteria. Bioactivity and genetic screening of actinobacteria associated with red algae Gelidiella acerosa were conducted to discover new antibacterial compounds against Vibrio alginolyticus. A total of 14 actinobacteria isolates were obtained from G. acerosa. The isolates were subjected to genetic screening for nrps (non-ribosomal peptide synthetase) and FADH₂-dependent halogenase genes. The isolates’ ability to produce secondary metabolites was examined by fermentation in various media in a six-well mini plate. The bioactivity of the secondary metabolites was screened using a microtiter assay and the agar overlay method. The results showed that all 14 isolates had the nrps gene, whereas none had the halogenase gene. Meanwhile, eight of the actinobacteria isolates showed antibacterial activity against V. alginolyticus.

Keywords

actinobacteria; antibiotic; FADH2-dependent halogenase; NRPS; red algae

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DOI: https://doi.org/10.22146/ijbiotech.25920