Isolation and caracterization of ficin enzyme from Ficus septica Burm F stem latex
Sri Wahyuni(1), R. Susanti(2*), Retno Sri Iswari(3)
(1) Biology Department of Mathematic and Science Faculty, Universitas Negeri Semarang, Semarang, Indonesia
(2) Biology Department of Mathematic and Science Faculty, Universitas Negeri Semarang, Semarang, Indonesia
(3) Biology Department of Mathematic and Science Faculty, Universitas Negeri Semarang, Semarang, Indonesia
(*) Corresponding Author
Abstract
Keywords
Full Text:
PDFReferences
Gagaoua, M., Boucherba, N.,Darenfed, A.B.,Ziane, F.,Rabah, S.N.,Hafd, K. and Boudechicha, H.R. 2014. Three-phase partitioning as an effcient method for the purifcation and recovery of fcin from mediterranean fg (Ficuscarica L. Latex). Separation and Purifcation Technology 132: 461-467.
Hames, B.D. and Hooper, N.M. 2000. Biochemistry: the instant notes. Hongkong: Springer Verlag.
Hasibuan, P.R.M., Alviyulita, M. and Hanum, F. 2014. Pengaruh penambahan natrium klorida (NaCl) dan waktu perendaman buffer fosfat terhadap perolehan crude papain dari daun pepaya (Carica papaya L.).Jurnal Teknik Kimia 3: 3. 39-44.
Herdyastuti, N. 2006. Isolasi dan karakterisasi ekstrak kasar enzim bromelin dari Batang nanas (Ananascomusus L.merr).Berk. Penelitian Hayati. 12: 75-77.
Iswanto, K.N., Sudarminto and Saparianti, E. 2009. Karakteristik aktivitas proteolitik enzim papain kasar (kajian zat pengaktif dan suhu pengeringan). [Laporan Penelitian]. Malang: Universitas Brawijaya Malang.
Layne, E. 1957. Spectrophotometric and turbidimetric methods for measuring proteins. Methods in Enzimology 3: 447-455.
Masfufatun. 2009.Isolasi dan karakterisasi enzim selulase. http://fk.uwks.ac.id/ archive/jurnal/vol. [23 April 2015]. Ngili, Y. 2010. Biokimia dasar. Bandung: Rekayasa Sains.
Perello, M., Arribere, M.C.,Caffni, N.O. and Priolo, N.S. 2000. Proteolytic enzimes from the latex of FicusPumila L. (Moraceae). Acta Farm Bonaerense 19 (4): 257-262.
Pourmorad, F.,Honary, S., Azadbakht, M., Asgarirad, H. and Golmohammadzadeh, G. 2011. Separation of proteolytic components of Iran ficus carica latex by column chromatography and electrophoresis and latex anticorn activity. International Journal of Pharmaceutical Sciences Review and Reasearch 1(7): 83-85.
Safriani, N., Basyamfar, R.A., and Bachtiar. 2011. Pengaruh konsentrasi ekstrak kasar enzim fsin (crude fcin) dan lama fermentasi terhadap rendemen dan kualitas minyak kelapa. Jurnal Teknologi dan Pertanian Indonesia 3(3): 12-19.
Sajuthi, D., Suparto, I., Yanti and Praira, W. 2010. Purifkasi dan pencirian enzim protease fbrinolitik dari ekstrak jamur merang. Makara Sains 14(2): 145-150.
Sebayang, F. 2006. Imobilisasi enzim papain dari getah pepaya dengan alginat. Jurnal Komunikasi Penelitian 18(2): 34-38.
Siota, L.F. and Villa, T.G. 2011. Native and biotechnology engineered plant proteases with industrial aplications. Food Biopress Tecnol. 4: 1066-1088.
Soeka, Y.S., Rahayu, S.H., Setianingrum, N. and Naiola, E. 2011. Kemampuan bacillus licheniformis dalam memproduksi enzim protease yang bersifat alkalin dan termofilik. Media Litbang Kesehatan
21(2): 89-95.
Walti, A. 1938. Crystalin fcin. J. Am. Chem. Soc. 40: 493.
Wardani, A.K. and Nindita, L.O. 2012. Purifkasi dan karakterisasi protease dari bakteri hasil isolasi dari Whey tahu. Jurnal Teknologi Pertanian 13(3): 149-156.
Witono, Y., Aulanni’am, Subagio, A. and Wijanarko, S.B. 2006. Pemurnian parsial enzim protease dari getah tanaman biduri (Calotropisgigantea) menggunakan ammonium sulphat. Jurnal Teknologi
Pertanian 7(1): 20-26.
DOI: https://doi.org/10.22146/ijbiotech.24200
Article Metrics
Abstract views : 4656 | views : 3937Refbacks
- There are currently no refbacks.
Copyright (c) 2017 Indonesian Journal of Biotechnology
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.