Serotipe virus dengue dan populasi Aedes aegypti dan Aedes albopictus di Kota Bengkulu: implikasi bagi program pencegahan demam berdarah

https://doi.org/10.22146/bkm.34730

Dessy Triana(1*), Sitti Rahmah Umniyati(2), Budi Mulyaningsih(3), Munauwarus Sarirah(4)

(1) Departemen Parasitologi Fakultas Kedokteran dan Ilmu Kesehatan Universitas Bengkulu
(2) Departemen Parasitologi Fakultas Kedokteran, Kesehatan Masyarakat dan Keperawatan Universitas Gadjah Mada
(3) Departemen Parasitologi Fakultas Kedokteran, Kesehatan Masyarakat dan Keperawatan Universitas Gadjah Mada
(4) Departemen Parasitologi Fakultas Kedokteran Universitas Islam Sumatera Utara
(*) Corresponding Author

Abstract


Dengue virus serotype and the population of Aedes aegypti and Aedes albopictus in Bengkulu City: its implications for dengue prevention programs 

Purpose: This study aimed to prevent the development of dengue virus by detecting dengue virus serotypes of Aedes aegypti (Ae. aegypti) and Aedes albopictus (Ae. albopictus) and to determine the population of Ae. aegypti and Ae. albopictus in dengue endemic area (Sidomulyo Village) and dengue sporadic area (Tanjung Jaya Village) in Bengkulu City, Indonesia. Methods: The design of study was observational-analytic. Aedes sp eggs were collected by ovitraps from Sidomulyo and Tanjung Jaya Villages. The eggs were reared to adult and identified to determine of Ae. aegypti and Ae. albopictus. Identification of species Ae. aegypti and Ae. albopictus uses the pictorial key for the identification of mosquitoes by Rueda. Detection of dengue virus serotypes were done by RT-PCR and nested PCR method using specific primers Lanciotti. Results: The Serotypes of dengue virus of Ae. aegypti in dengue endemic and sporadic areas were dengue-3 (DENV-3) and the serotypes of dengue virus of Ae. albopictus in dengue sporadic area was dengue-3 (DENV-3). The population ratio of Ae. aegypti and Ae. albopictus in dengue endemic area (61%:39%) and dengue sporadic areas (27%:73%), respectively. Conclusions: Aedes aegypti in dengue endemic and sporadic areas and Ae. albopictus in dengue sporadic area has potential as a dengue-3 vector. Health Agency of Bengkulu City could optimize the prevention program of dengue and activate the Jumantik cadres.

Abstrak

Tujuan: Penelitian ini bertujuan untuk pencegahan perkembangan penyakit DBD dengan mendeteksi serotipe virus dengue pada nyamuk Ae. aegypti dan Ae. albopictus dan menentukan rasio populasi nyamuk Ae. aegypti dan Ae. albopictus di daerah endemis dan sporadis DBD di Kota Bengkulu. Metode: Desain penelitian ini adalah observasional-analitik. Telur nyamuk Aedes sp dikumpulkan menggunakan ovitrap dari daerah endemis DBD (Kelurahan Sidomulyo) dan daerah sporadis DBD (Kelurahan Tanjung Jaya). Identifikasi spesies Ae. aegypti dan Ae. albopictus menggunakan pictorial key for the identification of mosquitoes Rueda. Deteksi serotipe virus dengue dilakukan dengan metode RT-PCR dan Nested PCR menggunakan primer spesifik Lanciotti. Hasil: Serotipe virus dengue yang ditemukan pada Ae. aegypti adalah DENV-3 (daerah endemis dan sporadis DBD) dan DENV-3 pada Ae. albopictus (daerah sporadis DBD). Perbandingan populasi Ae. aegypti dan Ae. albopictus pada daerah endemis dan sporadis DBD berturut-turut adalah (60,51%:39,49%) dan (27,08%:72,92%). Simpulan: Aedes aegypti di daerah endemis dan sporadis DBD serta Ae. albopictus di daerah sporadis DBD berpotensi sebagai vektor dengue-3. Dinas Kesehatan Kota Bengkulu dapat mengoptimalkan program pencegahan DBD dan mengaktifkan kader Jumantik.


Keywords


Aedes aegypti; Aedes albopictus; dengue virus serotypes



References

  1. WHO. Global Strategy for Dengue Prevention and Control 2012–2020. World Health Organization, 2012.
  2. Bhatt S, Gething PW, Brady OJ, Messina JP, Farlow AW, Moyes CL, et al. The global distribution and burden of dengue. Nature. 2013;496(7446): 504–507.
  3. Hawley WA. The biology of Aedes albopictus. Journal of the American Mosquito Control Association. 1988;12(1): 1–39.
  4. Sivan A, Shriram AN, Sugunan AP, Anwesh M, Muruganandam N, Kartik C, et al. Natural transmission of dengue virus serotype 3 by Aedes albopictus (Skuse) during an outbreak in Havelock Island: Entomological characteristics. Acta tropica. 2016;156: 122–129.
  5. Paupy C, Ollomo B, Kamgang B, Moutailler S, Rousset D, Demanou M, et al. Comparative Role ofAedes albopictusandAedes aegyptiin the Emergence of Dengue and Chikungunya in Central Africa. Vector-Borne and Zoonotic Diseases. 2010;10(3): 259–266.
  6. Kemenkes. INFODATIN (Situasi Demam Berdarah Dengue di Indonesia). Kementerian Kesehatan RI, 2016.
  7. Wakano F, Lazuardi L, Arguni E, Kusnanto H. Pola sebaran tingkat infeksi bersama serotipe virus dengue di wilayah kajian RT-PCR Balai Besar Teknik Kesehatan Lingkungan dan Pengendalian Penyakit Yogyakarta: analisis data 2013-2015. Berita Kedokteran Masyarakat. 2016;32(11): 401–408.
  8. Kusriastuti. Epidemiologi Penyakit Demam Berdarah Dengue dan Kebijakan Penanggulangannya di Indonesia. Simposium Dengue Control Up Date. 2005;
  9. Ritchie SA, N Hanna J, Hills SL, Piispanen JP, McBride H, John W, Pyke A, L Spark R. Dengue control in north Queensland, Australia: case recognition and selective indoor residual spraying. WHO Regional Office for South-East Asia, 2002.
  10. Lardo S, Utami Y, Yohan B, Tarigan SM, Santoso WD, Nainggolan L, et al. Concurrent infections of dengue viruses serotype 2 and 3 in patient with severe dengue from Jakarta, Indonesia. Asian Pacific journal of tropical medicine. 2016;9(2): 134–140.
  11. Loroño-Pino MA, Cropp CB, Farfán JA, Vorndam AV, Rodríguez-Angulo EM, Rosado-Paredes EP, et al. Common occurrence of concurrent infections by multiple dengue virus serotypes. The American journal of tropical medicine and hygiene. 1999;61(5): 725–730.
  12. Laille M, Deubel V, Sainte-Marie FF. Demonstration of concurrent dengue 1 and dengue 3 infection in six patients by the polymerase chain reaction. Journal of medical virology. 1991;34(1): 51–54.
  13. Mardihusodo SJ. Microplate Assay Analysis of Potential for Organophosphate Insectisida Resistance In Aedes aegypti In The Yogyakarta Municipality Indonesia. Berkala ilmu kedokteran. 1995;27: 71–79.
  14. Suroso. Dengue Haemorrhagic Fever in Indonesia: Epidemiological Trend and Development of Control Policy. Dengue bulletin. 1996;20: 35–40.
  15. Rueda LM. Pictorial keys for the identification of mosquitoes (Diptera: Culicidae) associated with Dengue Virus Transmission. Zootaxa. 2004;589(1): 1.
  16. Qiagen. RNeasy ® Mini Handbook. 2010.
  17. Lanciotti RS, Calisher CH, Gubler DJ, Chang GJ, Vorndam AV. Rapid detection and typing of dengue viruses from clinical samples by using reversetranscriptase-polymerase chain reaction. Journal of clinical microbiology. Journal of clinical microbiology. 1992;30(3): 545–551.
  18. Qiagen. QIAGEN One Step RT-PCR Handbook. 2012.
  19. Qiagen. HotStarTaq® PCR Handbook. 2010.
  20. Kemenkes. Pencegahan dan Pemberantasan Demam Berdarah Dengue di Indonesia. Kementerian Kesehatan RI, 2005.
  21. Ito M, Takasaki T, Kotaki A, Tajima S, Yuwono D, Rimal HS, et al. Molecular and virological analyses of dengue virus responsible for dengue outbreak in East Timor in 2005. Japanese journal of infectious diseases. 2010;63(3): 181–184.
  22. Satoto TBT, Umniyati SR, Astuti FD, Wijayanti N, Gavotte L, Devaux C, et al. Assessment of vertical dengue virus transmission in Aedes aegypti and serotype prevalence in Bantul, Indonesia. Asian Pacific Journal of Tropical Disease. 2014;4: S563–S568.
  23. Kukreti H, Mittal V, Chaudhary A, Rautela RS, Kumar M, Chauhan S, et al. Continued Persistence of a Single Genotype of Dengue Virus Type-3 (DENV-3) in Delhi, India Since its Re-emergence Over the Last Decade. Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi. 2010;43(1): 53–61.
  24. Araújo FM de C, de Carvalho Araújo FM, Nogueira RMR, de Araújo JMG, Ramalho ILC, de Sá Roriz MLF, et al. Concurrent infection with dengue virus type-2 and DENV-3 in a patient from Ceará, Brazil. Memorias do Instituto Oswaldo Cruz. 2006;101(8): 925–928.
  25. Urdaneta L, Herrera F, Pernalete M, Zoghbi N, Rubio-Palis Y, Barrios R, Rivero J, Comach G, Jiménez M, Salcedo M. Detection of dengue viruses in field-caught Aedes aegypti (Diptera: Culicidae) in Maracay, Aragua state, Venezuela by type-specific polymerase chain reaction. Infection, genetics and evolution: journal of molecular epidemiology and evolutionary genetics in infectious diseases. 2005;5(2): 177–184.



DOI: https://doi.org/10.22146/bkm.34730

Article Metrics

Abstract views : 7402 | views : 2094

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2018 Berita Kedokteran Masyarakat

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Berita Kedokteran Masyarakat ISSN 0215-1936 (PRINT), ISSN: 2614-8412 (ONLINE).

Indexed by:

 

Web Analytics Visitor Counter