The effect of mangan and lithium on peripheral blood mononuclear cell viability after exposure of narrowband ultraviolet B in psoriasis patients

Abstract

Psoriasis is a chronic and recurrent inflammation skin disease characterized with hyperproliferation of keratinocyte
mediated by T cell lymphocytes. Climatotherapy (bathing and sunbathing) at Dead Sea is model therapy which is
effective for moderate and severe psoriasis. However there are group of psoriasis patients who are not responsive
to climatotherapy. The success of the model therapy was based on the influence of ultraviolet irradiation which
causes depletion of lymphocytes, macrophages, and dendritic cells of the skin. Patients who are not respond to
climatotherapy shown to have higher level of mangan (Mn) and lithium (Li) serum than responsive patients. Mangan
and Li were suspected influenced the viability of peripheral blood mononuclear cells (PBMC). The aim of the study is
to evaluate the effect of Mn and Li levels on the viability of PBMC in the serum of psoriasis patient after exposure
of narrowband UVB (NB-UVB). The PBMC were isolated from 6 psoriasis patients using Histopaque. Then the cells
isolate wasincubated in RPMI medium with 0.02 to 0.08 μmol/LMn and 0.08 to 0.1 μmol/L Li for 3 days. After that
the cells were irradiated once with 1/3 to 2 minimal erythem dose (MED) of NB-UVB. The PBMC viability was
measured 3 hours after irradiation with MTT assay and read with ELISA plate reader. The results showed that
increased levels of Mn and Li or combination did not affect on the viability of PBMC at 1/3 to 1 MED UV-UVB. But
at higher irradiation dose (2 MED), the higher level of Mn and Li had negative affect in the viability of PBMC after
irradiation. It could be concluded that the Mn and Li level in the serum did not affect the viability of PBMC after
irradiation of NB-UVB. It was suggest that other cellular component that involved in the development of psoriasis
lesions, such as dendritic cells, fibroblasts, macrophages, and keratinocyte, were associated with Mn and Li levels.
Key words: psoriasis – mangan – lithium - peripheral blood mononuclear cell