The Expression of microRNA-155 and mRNA Hypoxia Inducible Factor Alpha (HIF1A) in the early and advanced stages of ovarian cancer patients blood plasma
S.N. Chasanah(1*), A.S. Fitriawan(2), F.K. Pukan(3), A.I. Kartika(4), R, Oktriani(5), A. Trirahmanto(6), H. Pradjatmo(7), T. Aryandono(8), S.M. Haryana(9)
(1) Basic Medical Science and Biomedicine Program, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
(2) Basic Medical Science and Biomedicine Program, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
(3) Basic Medical Science and Biomedicine Program, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
(4) Graduate Program in Biotechnology, School of Postgraduate, Universitas Gadjah Mada, Yogyakarta, Indonesia.
(5) Department of Biochemistry Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
(6) Department of Obstetry Gynecology, RSUP Dr. Sardjito, Yogyakarta, Indonesia.
(7) Department of Obstetry Gynecology, RSUP Dr. Sardjito, Yogyakarta, Indonesia.
(8) Department of Surgery, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
(9) Department of Histology and Cellular Biology, Faculty of Medicine, Universitas Gadjah Mada, Yogyakarta, Indonesia.
(*) Corresponding Author
Abstract
The aim of this study is to determine whether there are differences in the expression of miR-155 and mRNA HIF1A in plasma ovarian cancer patients at the early stage compared with the advanced stage.
The samples using blood plasma from ovarian cancer patients RSUP Dr. Sardjito with 32 ovarian cancer patients early stages and 20 ovarian cancer patients advanced stages. Total RNA was isolated from blood plasma samples of ovarian cancer patients. cDNA synthesis from total RNA was performed to obtain cDNA. The expression of miR-155 and HIF1A were calculated using qPCR. qPCR results were analyzed using Biorad CFX Manager Software.
The analysis showed that the expression of miR-155 were 2,18 times lower (p-value = 0,018*) in the plasma of advanced stage ovarian cancer compared with early stage, the differences were statistically significant (p value≤ 0,05). Whereas the mRNA expression HIF1A were 2,46 times higher (p-value = 0,039*) in the plasma of advanced stage ovarian cancer compared with early stage, the differences were statistically significant (p value≤ 0,05).
This study has proved that miR-155 expression is downregulated and followed by upregulation of mRNA expression HIF1A at an advanced stage ovarian cancer compared with early stage.
Keywords: Plasma, stage ovarian cancer, microRNA-155, mRNA HIF1A
Full Text:
PDFDOI: https://doi.org/10.19106/JMedScieSup004804201620
Article Metrics
Abstract views : 1304 | views : 1251Copyright (c) 2017 S.N. Chasanah, A.S. Fitriawan, F.K. Pukan, A.I. Kartika, R, Oktriani, A. Trirahmanto, H. Pradjatmo, T. Aryandono, S.M. Haryana
This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.