THE ACTIVITY OF RADICAL SCAVENGING OF 2,2-DIPHENYL-1-PYCRILHYDRAZIL (DPPH) BY ETHANOLIC EXTRACTS OF MENGKUDU LEAVES (Morinda citrifolia L.), BROTOWALI STEM ( Tinospora crispa L.), ITS WATER FRACTION AND ITS HYDROLIZED FRACTION

https://doi.org/10.22146/tradmedj.8850

Tatang Irianti(1*), Andayana Puspitasari(2), Machwiyyah L.(3), Rabbani HR.(4)

(1) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(2) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(3) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(4) Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author

Abstract


We have investigated the antiradical activity of ethanolic extract of mengkudu leaves (Morinda citrifolia L.) and brotowali stem (Tinospora crispa L.) using radical scavenging assay or DPPH radical. Thin layer chromatograms were also studied to estimate the group of compounds that have antiradical activity.  The objective of this research was to compare the DPPH antiradical efficiency values of water fraction and acid hydrolyzed water fraction in both extracts. The radical scavenging IC50 value of ethanolic extract, water fraction, and hydrolised water fraction (one and thee hours) of mengkudu leaves were 75,65 µg/mL ; 98,68 µg/mL ; 36,27 µg/mL and 33,36 µg/mL  respectively. The IC50 value for brotowali stem were 33.75 µg/mL, 52.29 μg/mL, 31.12 μg/mL and 18.26 μg/mL.  The highest antiradical activity was the hydrolyzed water fraction three hour namely 200 µg/mL of this fraction was able to inhibit 33.12%  and 18.26 μg/mL DPPH radicals.

Keywords


radical scavenging, Morinda citrifolia L.,Tinospora crispa, L., hydrolyzed water fraction, DPPH

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References

Amom Z., Bahari H., Isemaail S., Ismail NA., Shah Z.Md. dan Arsyad MS. 2009. Nutritional composition, Antioxidant Ability and Flavonoid Content of Tinospora crispa stem. Adv In Nat Appl Sci. 3 (1), 88-94.

Biesaga M. and Anna W.K.P., 2007, Food Chemistry, p. 699-704

Deng S., Brett J. West, C. Jarakae Jensen, 2008, Simultaneous Characterisation and Quantitation of Flavonol Glycosides and Aglycones in Noni Leaves Using a Validated HPLC-UV/MS Method, Food Chemistry, 111, 526–529.

Deng, Wangyuan, Guangzhong, 2011, A novel antioxidant activity index (AAU) for natural products using the DPPH assay, Food Chemistry 125, 1430–1435

Fuhram, B. & Aviram, M., 2002, Polyphenols and Flavonoids Protect LDL Against Atherogenic Modifications, dalam Cadenas, E., Packer, L., (Eds.), Handbook of Antioxidants, 303-336, Marcel Dekker Inc., New York.

Halliwell, B., R. Aeschbacht, J. Loligert dan O. I. Aruoma, 1995, The Characterization of Antioxidants, Fd Chem. Toxk, Vol. 33, No. 7, pp. 601-617

Harborne, J. B., 1965, Plant Polyphenols-XIV. Characterization of

Flavonoid Glycosides by Acidic and Enzymic Hydrolyses, Phytochemistry, vol 4, pp 107-120

Harborne, Jefrey B., Christine A. Williams, 2000, Review: Advances in flavonoid research since 1992, Phytochemistry 55, 481-504.

Heim, Kelly E., Anthony R. Tagliaferro, Dennis J. Bobilya, 2002, Flavonoid antioxidants: chemistry, metabolism and structure-activity relationships, Journal of Nutritional Biochemistry 13, 572–584

Hiranto dkk.,2001, Antioxidant ability of various flavonoids against DPPH radicals and LDL oxidation, J.of Nut. Science and Vitaminology, 47(5), 357-362.

Irianti T., Puspitasari, A., dan Choirunisa NA., (2012), Aktivitas Penangkapan Radikal 2,2-Difenil-1-pikrilhidrazil Oleh ekstrak Metanol Daun Mengkudu dan Fraksi-fraksinya, JBAI Vol.8 No. 2, hal. 92-101.

Irianti, T., Puspitasari, A. & Suryani, E., 2011, Aktivitas Penangkapan Radikal 2,2-Difenil-1-Pikrilhidrazil oleh Ekstrak Etanolik Batang Brotowali (Tinospora crispa (L.) Miers) dan Fraksi-fraksinya, Majalah Obat Tradisional, 16 (3), 138-144.

Jun, M.H.Y., Yu, J., Fong, X., Wan, C.S. & Yang, C.T., 2003, Comparison of Antioxidant Activities of Isoflavones from Kudzu Root (Pueraria labata Ohwl), J. Food Sci., 68, 2117-2122.

Kim, Gyo-Nam dan Hae-Dong Jang, 2010, Effect of Enzyme Treatment with β-Glucosidase on Antioxidant Capacity of Mulberry (Morus alba L.) Leaf Extract, Food Sci. Biotechnol. 19(5): 1341-1346

Macedo, J.A., dkk., 2011, Increasing the antioxidant power of tea extracts by biotransformation of polyphenols, Food Chemistry 126, 491–497.

Molyneux, Philips, 2004, The use of the stable free radical diphenylpicryl-hydrazyl (DPPH) for estimating antioxidant activity. Songklanakarin J Sci Technol 26(2):211–219

Nic´iforovic´, N, dkk., 2010, Antioxidant Activity of Selected Plant Species; Potential New Sources of Natural Antioxidants, Food and Chemical Toxicology, 48, 3125–3130

Pitchaon, M., 2011, Antioxidant capacity of extracts and fractions from mango (Mangifera indica Linn.) seed kernels, International Food Research Journal 18: 520-525

Sang, Shengmin., dkk., 2001, Flavonol Glycosides and Novel Iridoid Glycoside from the Leaves of Morinda citrifolia, J. Agric. Food Chem., 49, 4478−4481

Scherer, Rodrigo dan Helena Teixeira Godoy, 2009, Antioxidant activity index (AAI) by the 2,2-diphenyl-1-picrylhydrazyl method, Food Chemistry 112, 654–658

Sharma and Bhat, 2009, DPPH antioxidant assay revisited, Food Chemistry 113, p 1202–1205

Wach, Anna, Krystyna Pyrzynska, Magdalena Biesaga, 2007, Quercetin content in some food and herbal samples, Food Chemistry 100, 699–704

Wang, M.Y., dkk., 2002, Morinda citrifolia (Noni): A literature review and recent advances in Noni research, Acta Pharmacol Sin, 23 (1 2): 1127 -1141

West, B.J., Deng S. dan Jensen C.J., 2009, Nutrient and phytochemical analyses of processed noni puree, Food Res. Int. Doi: 9. 1016/ j.foodres. 2009.09.038

Wang SY., Kuo YH., Chang HN., Kang PL., Tsay HS., Lin KF., Yang NS., and Shyur NF.,2002, Profiling and Characterisation Antioxidant Activities In Anoectochilus Formosanus Hayata, J. Agric. Food. Chem., 50, 1859-1865

Zin dkk (2006), Antioxidative activities of chromatographic fractions obtained from root, fruit and leaf of Mengkudu (Morinda citrifolia L), Food Chemistry 94: 169-78.



DOI: https://doi.org/10.22146/tradmedj.8850

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