The Effect of Binahong Leaves (Anredera cordifolia Steenis) Extract on Blood Urea Nitrogen (BUN) Creatinine Serum and Renal Histopathology of Male White Rats (Rattus norvegicus) with Diabetes Mellitus

https://doi.org/10.22146/mot.85034

Joni Tandi(1*), Recky Patala(2), Dessy Gracelia Towulu(3), Putri Marcelita Lidongi(4), Tien Wahyu Handayani(5), Maria Kanan(6)

(1) Pelita Mas College of Pharmacy, Palu, Central Sulawesi
(2) Pelita Mas College of Pharmacy, Palu, Central Sulawesi
(3) Pelita Mas College of Pharmacy, Palu, Central Sulawesi
(4) Pelita Mas College of Pharmacy, Palu, Central Sulawesi
(5) Pelita Mas College of Pharmacy, Palu, Central Sulawesi
(6) Pelita Mas College of Pharmacy, Palu, Central Sulawesi
(*) Corresponding Author

Abstract


Diabetic nephropathy is one of the complications of diabetes mellitus on the pancreas that can end up in chronic pancreas failure. Various treatment options to repair pancreas damage due to diabetic nephropathy, one of which is by using Anredera cordifolia leaves. This study aims to determine the content of secondary metabolites in Anredera cordifolia leaves ethanol extract, and to determine the effective dose of Anredera cordifolia leaves ethanol extract in regenerating male white rat kidney cells. This study uses a laboratory experimental method. This study used 30 rats divided into 6 treatment groups, each group consisting of 5 test animals, namely normal group, negative control, positive control, dose of 25 mg/kg BW, dose 50 mg/kg BW, and dose 100 mg/kg bw. The level of histological damage to the renal tubules was observed with HE staining using an Olympus CX23 microscope. The data from the scoring of the level of renal tubular damage were analyzed using the Kruskal-Wallis test followed by the Mann-Whitney test to see the differences between treatments. The results showed that the Anredera cordifolia leaves ethanol extract contained secondary metabolites of alkaloids, flavonoids, saponins, and tannins; Anredera cordifolia leaves ethanol extract has an effect on kidney cell repair; Anredera cordifolia leaves ethanol extract at a dose of 100 mg/kg bw is effective reducing urea and creatinine; with an average decrease of 17.0 and 0.71 mg/dL in repairing kidney cells with an average damage value of 1.


Keywords


Anredera cordifolia leaves; Kidney Histopathology; Urea and Creatinin Male White Rat

Full Text:

PDF


References

Amir, N., Suprayitno, E., Hardoko, H., dan Nursyam, H. (2015). ‘Pengaruh Sipermetrin Pada Jambal Roti Terhadap Kadar Ureum Dan Kreatinin Tikus Wistar ( Rattus norvegicus )’, Ipteks Psp, 2(3), pp. 283–293.

Andrie, M., Wintari, T., & Rizqa, A. (2014). Activities Test Of “Jamu Gendong Kunyit Asam” (curcuma domestica Val.; Tamarindus indica L.) As an Antidiabetic In Streptozotocin-Induced Rats. Traditional Medicine Journal, 19(2), 95–102.

Asikin, 2018). GambaranTingkat PengetahuanPenyakit Ginjal Dan Terapi Diet Ginjal Dan Kualitas Hidup Pasien Hemodialisis DiRumkital Dr. Ramelan Surabaya.

Aji, N. P.,Bachri,M.S.,& Khasanah,N (2019). Efek Kombinasi Ektrak Etanol Herba Sambiloto (Andrographis Paniculata) Dan Daun Kelor (Moringa Oleifera) Terhadap Kadar Ureum dan Kreatinin pada tikus wistar yang Diinduksi Streptozotocin.Jurnal Akademi Farmasi Prayoga, 1(1), 13-18

Bisala, F. K., Fitiyani Ya, U., studi, P. S., & Pelita Mas Palu, S. (2019). Uji Efek Antidiabetes Ekstrak Etanol Daun Talas Pada Tikus Putih Jantan Hiperkolesterolemia-Diabetes. Farmakologika Jurnal Farmasi, XVI(1), p.

Dessy Hariyanti, L. P., N Wisnu Wardhana, A. A., Sri Indriyani, N. K., & P Yudia Putri, I. A. (N.D.) 2017. Pengaruh Pemberian Ekstrak Etanol Daun Binahong (Anredera Scandens (L.) Moq.) Terhadap Histopatologi Hati Mencit Jantan Galur Balb/C Yang Diinduksi Dengan Karbon Tertraklorida.

Luna LG (1968). Manual of Histologic Staining Methods of the Armed Forces Institute of Pathology 3rd Edition. American Registry of Pathology. McGraw-Hill Book Company. New York.

Tandi, J., Wulandari, A., Tandi ProgramStudiS, J., & STIFA Pelita Mas, F. (2017). Efek Ekstrak Etanol Daun Gendola Merah (Basella alba L.) terhadap Kadar Kreatinin. Galenika Journal of Pharmacy), 3(2), 93–102. https://doi.org/10.22487/j24428744.2017.v3.i2.8813

Tandi, J., Roem, M., & Yuliet, Y. (2017). Efek Nefroprotektif Kombinasi Ekstrak Daun Gedi Merah dan Daun Kumis Kucing pada Tikus Induksi Etilen Glikol. Journal Of Tropical Pharmacy And Chemistry, 4(1), 27–34. https://doi.org/10.25026/jtpc.v4i1.129

Tandi, J., Wulandari, A., & Asrifa, A. (2017). Efek Ekstrak Etanol Daun Gendola Merah (Basella alba L.) terhadap Kadar Kreatinin, Ureum dan Deskripsi Histologis Tubulus Ginjal Tikus Putih Jantan (Rattus norvegicus) Diabetes yang Diinduksi Streptozotocin. Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-Journal), 3(2), 93–102. https://doi.org/10.22487/j24428744.0.v0.i0.8813

Tandi, J. (2016). Uji Efek Ekstrak Etanol Daun Gedi Merah (Abelmoschus manihot (L.) Medik) Tikus Putih Jantan(Rattus norvegicus) Diabetes Mellitus Tipe 2. International Journal Of Sciences, 30(4), 84–94.

Tandi, J., Nugraha, F. R., & Afandi, W. N. (2020a). Potensi Nefroterapi Daun Nangka (Artocarpus heterophyllus Lamk) Terhadap Tikus Putih Diabetes Mellitus. Jurnal Farmasi Udayana. 213.

Tandi, J, Dewi , N. P., Wirawan, R. C., & Surat, M. R (2020b). Potensi Rumput Laut (EuCheumea cottonii J.Agardh) Terhadap Nefropati Diabetik Tikus Putih Jantan (Rattus norvegicus). Jurnal Farmasi Galenika (Galenika Journal of Pharmacy) (e-jaurnal). 6: 286-294.

Tandi J. 2017. Efek Ekstrak Etanol Daun Gendola Merah (Basella alba L.) Terhadap Kadar Kreatinin, Ureum dan Deskripsi Histologis Tubulus GinjalTikus Putih Jantan Yang diinduksi Streptozotocin. Jurnal Farmasi. 3: 93-102

Tandi, J. 2018. Analisis Daun Gedi Merah (Abelmoschus manihot (L) medik) sebagai Obat Diabetes. Mellitus Buku Kedokteran EGC. 8–16.

Tandi, J. (2020). Farmakologika Jurnal Farmasi Vol . No. Maret 2020 Farmakologika Jurnal Farmasi Vol . No. Maret 2020 Diabetes melitus merupakan suatu.

Tandi, J. (2017). Pengaruh Ekstrak Etanol Daun Jamu Air( Syzygium aqueum ( Burm f .) Alston ) terhadap Glukosa Darah, Urem Dan Kreatinin Tikus Putih ( Rattus norvegicus ). Tropical Farmasi And Chemistry, 4(2), 43–51.

Tandi, J., As, S., Natzir, R., & Bukhari, A. (2016). Test of Ethanolextract Red Gedi Leaves ( Albelmoschus Manihot . ( L .) Medik ) in White Iat ( Rattus Norvegicus ) Type 2 Diabetes Mellitus. International Journal Of Sciences, 30(4), 84–94.

Tandi, J., Roem, M., & Yuliet. (2017). Efek Nefroprotektif Kombinasi Ekstrak Daun Gedi Merah dan Daun Kumis Kucing pada Tikus Induksi Etilen Glikol. J.Trop Pharm, 4(1), 27–34.

Tandi, J., Said, N. A. and Kenta, Y. S. (2019) ‘Efek Ekstrak Etanol Kulit Batang Ketapang terhadap Gambaran Histopatologi Pankreas Tikus Putih Jantan’, Farmakologika Jurnal Farmasi, XVI(1), pp. 59–68

Tandi, J. et al. (2017) ‘Uji Efek Ekstrak Etanol Daun Sukun (Artocarpus altilis (Parkinson Ex F.A.Zorn) terhadap Penurunan Kadar Glukosa Darah, Kolestrol Total dan Gambaran Histopatologi Pankreas Tikus Putih Jantan (Rattus norvegicus) Hiperkolestrolemia-Diabetes’, Jurnal Sains dan Kesehatan, 1(8), pp. 384–396.

Tandi, J. (2018). Buku Ajar Obat Tradisional (R. Pitopang, Astija, T. Miting, A.

Tandi, J. (2018a) ‘Efek Ekstrak Biji Labu Kuning Terhadap Glukosa, Kolesterol dan Gambaran Histopatologi Pankreas Tikus Hiperkolesterolemia-Diabetes’, Talenta Conference Series: Tropical Medicine (TM), 1(3), pp. 144–151.

Tandi, J., Na’i, A. and Basilingan, A. (2019) ‘Uji Efek Kombinasi EEDS dan DPW terhadap Penurunan Kadar Kolesterol Total dan Glukosa Darah Tikus Putih Jantan Hiperkolesterolemia- Diabetes’, Pharmacy Medical journal, 2(1), pp. 8–27.

Tandi J., Danthy, R., Purwaningsih, dan Kuncoro, H. (2019). Effect Of Ethanol Extract From Purple Eggplant Skin (Solanum melongena L.) On Blood Glucose Levels and Pancreatic B Cells Regeneration On White Rats Male Hypercholesterolemia-Diabetic. Research Journal Of Pharmacy and Technology, 12(6), 2936-2942.

Tandi J., Niluh, P. D., Kiki, R. H., Resky, C. W., dan Megawati, R. S. (2020). Potensi Rumput Laut (Eucheuma Cottonii J.Agardh) Terhadap Nefropati Diabetik Tikus Putih Jantan (Rattus Norvegicus). Jurnal Farmasi Galenika (Galenika Journal Of Pharmacy) (E- Journal), 6(2), 286–294.

Tandi J, Nugraha,F.R.,Afandi,W.N.(2020).Potensi Nefroterapi Daun Nangka ( Artocarpus heterophyllus lamk) Terhadap Tikus Putih Diabetes Mellitus. Jurnal Farmasi Udayana,9(3), 204-212

Nurtika.2017.UJI ANTIDIABETIK DAUN BINAHONG (Anredera Cordifolia (Ten.) Pada Tikus Putih Jantan Galur Wistar (Rattus norvegicus)Yang Diinduksi Aloksan. Program Studi Pendidikan Dokter Fakultas Kedokteran Universitas Muhammadiyah Surakarta )

Putra, H. W. (2019). Sistem Pakar Diagnosis Penyakit Ginjal Dengan Metoda Forward Chaining. Jurnal Sains Dan Informatika, 5(1), 7. https://doi.org/10.22216/jsi.v5i1.4081

Puspitaningrum, L. S., Tjahjono, K., & Candra, A. (2018). Pengaruh Pemberian Ekstrak Daun Kelor (Moringa Oleifera) Terhadap Kadar Ureum Dan Kreatinin Serum Tikus Wistar Yang Diinduksi Formalin. Diponegoro Medical Journal (Jurnal Kedokteran Diponegoro), 7(2), 777–786.



DOI: https://doi.org/10.22146/mot.85034

Article Metrics

Abstract views : 1088 | views : 956

Refbacks

  • There are currently no refbacks.


Bookmark and Share


Copyright (c) 2023 Majalah Obat Tradisional

Creative Commons License
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

©Majalah Obat Tradisional (Traditional Medicine Journal)
 ISSN 2406-9086
Faculty of Pharmacy
Universitas Gadjah Mada